Anti insulin

Manufactured by Agilent Technologies

Sourced in United States, Denmark, United Kingdom

Anti-insulin is a laboratory reagent used to measure insulin levels in biological samples. It is a specific antibody that binds to insulin, allowing for the quantification of insulin concentration through immunoassay techniques.

Automatically generated - may contain errors

Lab products found in correlation

Anti glucagon, by Merck Group (7 mentions) Anti glucagon, by Agilent Technologies (5 mentions) 4 6 diamidino 2 phenylindole (dapi), by Merck Group (4 mentions) Anti pdx1, by Abcam (3 mentions) Anti glut2, by Merck Group (3 mentions) Fluorescein isothiocyanate fitc or cy3 conjugated secondary antibodies, by Jackson ImmunoResearch (3 mentions) Nis elements software, by Nikon (3 mentions) Vectashield with 4 6 diamidino 2 phenylindole dapi, by Vector Laboratories (3 mentions) Mea53200, by Nikon (3 mentions) Anti cleaved caspase 3, by BD (3 mentions) Anti glucagon, by Abcam (3 mentions) Anti sox17, by R&D Systems (2 mentions) Anti pdx1, by R&D Systems (2 mentions) Paraformaldehyde solution, by Thermo Fisher Scientific (2 mentions) Anti liprin alpha1, by Proteintech (2 mentions) Anti p mst1, by Cell Signaling Technology (2 mentions) Anti bim, by Cell Signaling Technology (2 mentions) Lsm 510, by Zeiss (2 mentions) Lsm800 confocal microscope, by Zeiss (2 mentions) Lsm 880, by Zeiss (2 mentions)

Close spelling variants of this product

Guinea pig anti-insulin antibody Polyclonal guinea pig anti-porcine insulin Guinea pig anti-swine insulin Polyclonal guinea pig anti-insulin Anti-insulin antibody Guinea pig anti-insulin Polyclonal guinea pig anti-insulin antibody Anti-swine insulin antibody Guinea pig anti-insulin polyclonal Ab Guinea pig anti-swine insulin antibody

46 protocols using anti insulin

Multicolor Immunofluorescence Staining

Check if the same lab product or an alternative is used in the 5 most similar protocols

Slides were retrieved and air dried prior to staining. After rinsing in 1x Tris buffered saline (TBS), they were blocked with 1x TBS / 1% bovine serum albumin/10% FBS/0.3 M glycine for 1 hr at room temperature. Slides were then stained with anti-phospho-histone γH2AX (Merck), anti-53bp1 (Bethyl Laboratories) and anti-Insulin (Dako) overnight at +4 °C. Next, the slides were rinsed and incubated with secondary antibodies, anti-rabbit Alexa 488, anti-rabbit Alexa 647, anti-mouse Alexa 488, anti-guinea pig Alexa 547 (Life Technologies) and DAPI for 1 hour at room temperature. Finally, slides were rinsed in 1x TBS before being mounted with Hardset Antifade mounting medium (Vectashield) and a glass coverslip.

Tay V.S., Devaraj S., Koh T., Ke G., Crasta K.C, & Ali Y. (2019). Increased double strand breaks in diabetic β-cells with a p21 response that limits apoptosis. Scientific Reports, 9, 19341.

+ Open protocol

+ Expand

Pancreatic Immunohistochemistry of Mouse

Check if the same lab product or an alternative is used in the 5 most similar protocols

Mouse pancreases were dissected and fixed in 4% formaldehyde at 4 °C for 12 h before embedding in paraffin^{62 (link)}. Two-four-micrometer sections were deparaffinized, rehydrated, and incubated overnight at 4 °C with anti-PDX-1 (Abcam; #47267), anti-Glut2 (Chemicon; #07-1402), anti-Ki67 (Dako; #M7249), anti-phospho-Histone H3 (Ser10; Merck #06-570), and anti-NKX6.1 (DSHB, University of Iowa #F55A12^{66 (link)}) in combination with TSA (Invitrogen #T30955), or for 2 h at room temperature with anti-insulin (Dako; A0546) antibodies (all at a dilution of 1:100, except anti-PDX-1, which was diluted 1:400) followed by fluorescein isothiocyanate (FITC)- or Cy3-conjugated secondary antibodies (Jackson ImmunoResearch Laboratories, West Grove, PA). Slides were mounted with Vectashield with 4′6-diamidino-2-phenylindole (DAPI) (Vector Labs). β-cell apoptosis was analyzed by the terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling (TUNEL) technique according to the manufacturer’s instructions (In Situ Cell Death Detection Kit, TMR red; Roche) and double stained for insulin. Fluorescence was analyzed by using a Nikon MEA53200 (Nikon GmbH, Dusseldorf, Germany) microscope, and images were acquired by using NIS-Elements software (Nikon).

Ardestani A., Li S., Annamalai K., Lupse B., Geravandi S., Dobrowolski A., Yu S., Zhu S., Baguley T.D., Surakattula M., Oetjen J., Hauberg-Lotte L., Herranz R., Awal S., Altenhofen D., Nguyen-Tran V., Joseph S., Schultz P.G., Chatterjee A.K., Rogers N., Tremblay M.S., Shen W, & Maedler K. (2019). Neratinib protects pancreatic beta cells in diabetes. Nature Communications, 10, 5015.

+ Open protocol

+ Expand

Pancreatic Islet Morphometry and Ultrastructure

Check if the same lab product or an alternative is used in the 5 most similar protocols

Islet area: 10–12 pancreatic sections (5 μm) separated by 200 μm were stained with H&E and area of individual circled islets calculated using Matlab. β- and α-cells were stained using anti-insulin (1:100, Dako) or anti-glucagon (1:50, Sigma). EM: whole islets were fixed and embedded in Epon. Thin sections (60 nm) were viewed by Tecnai 12 transmission electron microscope at 120 kV. Mitochondrial surface area was calculated from 250 single mitochondria (∼30 β-cells).

Oropeza D., Jouvet N., Bouyakdan K., Perron G., Ringuette L.J., Philipson L.H., Kiss R.S., Poitout V., Alquier T, & Estall J.L. (2015). PGC-1 coactivators in β-cells regulate lipid metabolism and are essential for insulin secretion coupled to fatty acids. Molecular Metabolism, 4(11), 811-822.

+ Open protocol

+ Expand

Immunocytochemical Analysis of Pancreatic Progenitors

Check if the same lab product or an alternative is used in the 5 most similar protocols

The sample cells were fixed in 1% paraformaldehyde and blocked with 3% BSA and 0.2% triton X-100 in PBS, and then incubated with primary antibody over night at 4 ^oC and further incubated with secondary antibody (Alexa Fluor 488-or 568-conjugated donkey or goat antibodies directed against mouse, goat, rabbit or guinea pig IgG at 1:400 dilutions). The used primary antibodies induce anti-FOXA2 (rabbit IgG, 1:200, GeneTex), anti-SOX17 (mouse IgG, 1:200, R&D Systems), anti-PDX1 (goat IgG, 1:300, R&D Systems) and anti-insulin (guinea-pig IgG, 1:300, Dako). Nuclei were stained with DAPI (4,6-diamidino-2-phenylindole). Images were captured using the LEICA DMI6000B and the positive cells were counted and analyzed by Fiji imaging software [21 (link)].

Liu H., Guo D., Ruzi A., Chen Y., Pan T., Yang F., Li J., Xu K., Zhou T., Qin D, & Li Y.X. (2017). Testosterone improves the differentiation efficiency of insulin-producing cells from human induced pluripotent stem cells. PLoS ONE, 12(6), e0179353.

+ Open protocol

+ Expand

Pancreatic CB1 Receptor Regulation

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

Animal care and procedures were approved by the National Institute on Aging Animal Care and Use Committee.Mice were housed in groups of four using 12 h dark/light cycles, provided with water and fed ad libitum. Cnr1^flox/flox mice were generated as described in the ESM Methods. Cnr1^flox/flox mice were mated with MIP-Cre/ERT mice (University of Chicago, Chicago, IL, USA) and injected daily for 5 days with i.p. tamoxifen to obtain β-CB1R^−/− mice (Cnr1^flox/flox:MIP-Cre/ERT⁺), β-CB1R^+/+ control littermates (Cnr1^flox/flox:MIP-Cre/ERT⁻) and MIP-Cre/ERT mice (Cnr1^wt/wt:MIP-Cre/ ERT⁺). CB1KO mice (NIH, Bethesda, MD, USA) were bred as described in the ESM Methods. Male mice (n = 6–7 mice/group) were aged to 25 weeks and body weights and metabolic variables were analysed. Body composition was analysed using NMR. Pancreases were fixed and processed for immunohistochemistry (anti-insulin [1:100; Dako], antiglucagon [1:500; Sigma-Aldrich], anti-BrdU [1:100; Accurate Chemicals]). Islet size was quantified using Pancreas++ [25 (link)]. Hormones were quantified using ELISA. Methanol–chloroform-extracted ECs from plasma were analysed using LC-MS/MS, as described in the ESM Methods.

González-Mariscal I., Montoro R.A., Doyle M.E., Liu Q.R., Rouse M., O’Connell J.F., Santa-Cruz Calvo S., Krzysik-Walker S.M., Ghosh S., Carlson O.D., Lehrmann E., Zhang Y., Becker K.G., Chia C.W., Ghosh P, & Egan J.M. (2018). Absence of cannabinoid 1 receptor in beta cells protects against high-fat/high-sugar diet-induced beta cell dysfunction and inflammation in murine islets. Diabetologia, 61(6), 1470-1483.

+ Open protocol

+ Expand

Pancreatic Alpha and Beta Cell Quantification

Check if the same lab product or an alternative is used in the 5 most similar protocols

Pancreata were weighed, fixed in 10% neutral buffered formalin solution for 48 h, and then embedded in paraffin. Two sections of the pancreas from each animal were stained with an anti-glucagon (REGN745, an anti-glucagon monoclonal antibody generated at Regeneron) or an anti-insulin (Dako) antibody and areas of glucagon and insulin positive cells were measured using Halo digital imaging analysis software (Indica Labs). The per cent of glucagon and insulin positive areas in proportion to the whole pancreas area were calculated. Cell mass was determined by multiplying the alpha cell or beta cell area for each animal against their pancreas weight.

Latres E., Mastaitis J., Fury W., Miloscio L., Trejos J., Pangilinan J., Okamoto H., Cavino K., Na E., Papatheodorou A., Willer T., Bai Y., Hae Kim J., Rafique A., Jaspers S., Stitt T., Murphy A.J., Yancopoulos G.D, & Gromada J. (2017). Activin A more prominently regulates muscle mass in primates than does GDF8. Nature Communications, 8, 15153.

+ Open protocol

+ Expand

Cellular Localization of ZIP6 and ZIP7

Cited 2 times

Check if the same lab product or an alternative is used in the 5 most similar protocols

The cellular localization of ZIP6 and ZIP7 was determined in primary dispersed mouse islet cells and MIN6 β cells using confocal microscopy. Staining was performed as described previously (6 (link)) with primary anti-HA (1:1000, Covance Inc.), anti-FLAG (1:500, Sigma), anti-KDEL (1:200, Pierce, ThermoFisher), anti-Syntaxin-1a (1:200, Sigma), anti-ZIP7 (1:200, Proteintech, Chicago, IL), anti-ZIP6-Y3 (1:20, an antibody generated in-house by Kathryn M. Taylor, Cardiff University, UK (19 (link))), or anti-insulin (1:100, Dako) primary antibody, followed by Alexa Fluor 488 goat anti-mouse (1:500, Molecular Probes, Life Technologies), Alexa Fluor 555 donkey anti-rabbit (1:500, Molecular Probes, Life Technologies), or donkey anti-guinea pig (1:500, Jackson ImmunoResearch Laboratories) secondary antibodies. Images were acquired on Zeiss confocal microscope at ×40 magnification with an oil lens and analyzed by LSM510 (Zeiss). Colocalization of ZIP6 and ZIP7 with membrane and ER staining was analyzed and determined with Volocity software.

Liu Y., Batchuluun B., Ho L., Zhu D., Prentice K.J., Bhattacharjee A., Zhang M., Pourasgari F., Hardy A.B., Taylor K.M., Gaisano H., Dai F.F, & Wheeler M.B. (2015). Characterization of Zinc Influx Transporters (ZIPs) in Pancreatic β Cells: ROLES IN REGULATING CYTOSOLIC ZINC HOMEOSTASIS AND INSULIN SECRETION. The Journal of Biological Chemistry, 290(30), 18757-18769.

+ Open protocol

+ Expand

Comprehensive Immunohistochemistry and Western Blot Antibody Panel

Check if the same lab product or an alternative is used in the 5 most similar protocols

The following antibodies were used in IHC and IF: guinea pig anti-insulin (DAKO, #A0564, 1:1,000), rabbit anti-glucagon (Phoenix, #H-028–05, 1:2,000), rabbit anti Ki67 (Abcam, #ab16667, 1:200), rabbit anti-cleaved caspase 3 (Cell Signaling, #9661, 1:200), rat anti-BrdU (Abcam, #ab6326, 1:200), mouse anti-Nkx6–1 (Hybridoma Bank, #F55A12, 1:100) rabbit anti-Mafa (Bethyl, #IHC-00352, 1:100), rabbit anti-Nkx2–2 (Sigma, #HPA003468, 1:100), rabbit anti-ALDH1A3 (Novus, #NBP2–15339, 1:100) and rabbit anti-gastrin (Millipore, #256A, 1:200). The following antibodies were used for western blot: rabbit anti-cleaved caspase 3 (Cell Signaling, #9661, 1:1,000), rabbit anti-adipsin (Santa Cruz, #sc-50419, 1:1,000), chicken anti-C3a (Abcam, #ab48581, 1:1,000), rabbit anti-Dusp26 (Invitrogen, #PA5–22013, 1:1,000), rabbit anti-actin (Cell Signaling, #8456, 1:1,000) and mouse anti-FLAG (Sigma, #A8592, 1:1,000). Recombinant C3a (R&D) was used at a concentration of 100 nM. NSC-87877 compound was dissolved in PBS and used at 20 μM concentration.

Gómez-Banoy N., Guseh J.S., Li G., Rubio-Navarro A., Chen T., Poirier B., Putzel G., Rosselot C., Pabón M.A., Camporez J.P., Bhambhani V., Hwang S.J., Yao C., Perry R.J., Mukherjee S., Larson M.G., Levy D., Dow L.E., Shulman G.I., Dephoure N., Garcia-Ocana A., Hao M., Spiegelman B.M., Ho J.E, & Lo J.C. (2019). Adipsin preserves beta cells in diabetic mice and associates with protection from type 2 diabetes in humans. Nature medicine, 25(11), 1739-1747.

+ Open protocol

+ Expand

Immunohistochemical Analysis of Grafted Kidney Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

Mouse kidneys with cells grafted under the capsules were washed with PBS, fixed with 4% paraformaldehyde at 4 °C overnight, and transferred to 30% sucrose solution for dehydration. The tissues were embedded in a 2:1 mixture of OCT: 30% sucrose and sectioned using a cryostat microtome. The slides were blocked and permeabilized in PBS solution containing 5% horse serum and 0.3% Triton for 1 h at RT and then incubated with primary antibodies overnight at 4 °C followed by 1 h incubation with fluorescence-conjugated secondary antibodies (Alexafluor, ThermoFisher Scientific) at RT. The following primary antibodies were used: anti-PDX1 (1:500, R&D), anti-insulin (1:500, DAKO) and anti-cleaved caspase-3 (1:1000, BD Biosciences), and anti-STEM121 (1:1000, Stem Cells Inc.). Fluorescent images were scored using MetaMorph® image analysis software (Molecular Devices).

Amin S., Cook B., Zhou T., Ghazizadeh Z., Lis R., Zhang T., Khalaj M., Crespo M., Perera M., Xiang J.Z., Zhu Z., Tomishima M., Liu C., Naji A., Evans T., Huangfu D, & Chen S. (2018). Discovery of a drug candidate for GLIS3-associated diabetes. Nature Communications, 9, 2681.

+ Open protocol

+ Expand

Dual Fluorescence Staining of Pancreas Islet Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

Pancreas tissue was fixed in buffered 10% formalin, processed to wax blocks and sectioned onto slides (4 μm). Dual fluorescence staining was performed to determine the islet cell type in which β-galactosidase/GPR120 was expressed. Anti-insulin (1:500; Dako, Cambridge, UK), anti-glucagon (1:200; Abcam, Cambridge, UK) and anti-somatostatin (1:100; Millipore, Watford, UK) were detected using Alexa Fluor-488 conjugated anti-goat antibody (Abcam) and anti-β-galactosidase (1:1200; Abcam) with Alexa Fluor-568 conjugated anti-rabbit antibody. Citrate buffer (pH 6) was used for antigen retrieval in the case of β-galactosidase and somatostatin. Briefly, tissue sections were fixed, de-waxed and rehydrated prior to antigen retrieval (heating in a microwave at full power for 20 min followed by cooling to room temperature). The sections were blocked before the addition of primary antibodies and washed prior to the addition of secondary antibodies and DAPI nuclear stain (1:500; Thermo Scientific). Slides were mounted in Hardset Vectashield Mounting medium (Vector Laboratories, Peterborough, UK) and analysed on a Nikon Eclipse 90i Fluorescent Microscope.

Stone V.M., Dhayal S., Brocklehurst K.J., Lenaghan C., Sörhede Winzell M., Hammar M., Xu X., Smith D.M, & Morgan N.G. (2014). GPR120 (FFAR4) is preferentially expressed in pancreatic delta cells and regulates somatostatin secretion from murine islets of Langerhans. Diabetologia, 57(6), 1182-1191.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!