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Acetylated histone 3 ac h3

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Acetylated histone 3 (Ac-H3) is a post-translationally modified form of the histone protein H3. Histones are responsible for the structural organization of DNA within the nucleus of eukaryotic cells. Ac-H3 is characterized by the acetylation of specific lysine residues on the histone H3 protein, which can influence chromatin structure and gene expression.

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Lab products found in correlation

Hnf4a, by Santa Cruz Biotechnology (2 mentions) Acetylated histone 4 ac h4, by Merck Group (2 mentions) Pcr clean up extraction kit, by Macherey-Nagel (2 mentions) Chrebp, by Novus Biologicals (2 mentions) Normal rabbit immunoglobulin g igg antibody, by Santa Cruz Biotechnology (2 mentions)

2 protocols using acetylated histone 3 ac h3

1

ChIP Analysis of Liver Histone Modifications

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Chromatin immunoprecipitation (ChIP) analysis was performed as described,23 with the following modifications. Before cross‐linking with 1% formaldehyde, livers were homogenized in PBS and cross‐linked with 0.5 M of di(N‐succinimidyl) glutarate for 45 minutes at room temperature. Immunoprecipitation of samples was performed overnight at 4°C with 3 µg of ChREBP (Novus), acetylated histone 4 (Ac‐H4; Millipore), acetylated histone 3 (Ac‐H3; Millipore), HNF4A (Santa Cruz), or normal rabbit immunoglobulin G (IgG) antibody (Santa Cruz). DNA was purified using the PCR Clean‐up Extraction Kit (Macherey‐Nagel), after which qPCR was performed. Primers are listed in Supporting Table S7.
Hoogerland J.A., Lei Y., Wolters J.C., de Boer J.F., Bos T., Bleeker A., Mulder N.L., van Dijk T.H., Kuivenhoven J.A., Rajas F., Mithieux G., Haeusler R.A., Verkade H.J., Bloks V.W., Kuipers F, & Oosterveer M.H. (2019). Glucose‐6‐Phosphate Regulates Hepatic Bile Acid Synthesis in Mice. Hepatology (Baltimore, Md.), 70(6), 2171-2184.
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2

Chromatin Immunoprecipitation Assay with Modifications

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Chromatin immunoprecipitation (ChIP) analysis was performed as described,(23 (link)) with the following modifications. Before cross-linking with 1% formaldehyde, livers were homogenized in PBS and cross-linked with 0.5 M of di(N-succinimidyl) glutarate for 45 minutes at room temperature. Immunoprecipitation of samples was performed overnight at 4°C with 3 μg of ChREBP (Novus), acetylated histone 4 (Ac-H4; Millipore), acetylated histone 3 (Ac-H3; Millipore), HNF4A (Santa Cruz), or normal rabbit immunoglobulin G (IgG) antibody (Santa Cruz). DNA was purified using the PCR Clean-up Extraction Kit (Macherey-Nagel), after which qPCR was performed. Primers are listed in Supporting Table S7.
Hoogerland J.A., Lei Y., Wolters J.C., de Boer J.F., Bos T., Bleeker A., Mulder N.L., van Dijk T.H., Kuivenhoven J.A., Rajas F., Mithieux G., Haeusler R.A., Verkade H.J., Bloks V.W., Kuipers F, & Oosterveer M.H. (2019). Glucose‐6‐Phosphate Regulates Hepatic Bile Acid Synthesis in Mice. Hepatology (Baltimore, Md.), 70(6), 2171-2184.
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