Antibodies used in this work include: Cetuximab (Creative Diagnostics Cat# AIT-19034, RRID:AB_2489605; Erbitux), anti-Erk1/2 (Cell Signaling Technology Cat# 4695, RRID:AB_390779), anti-phospho-ERK1/2 (T202/Y204) (Cell Signaling Technology Cat# 4370, RRID:AB_2315112), anti-p38 (Cell Signaling Technology Cat# 8690, RRID:AB_10999090), anti-phospho-p38 (T180/Y182) (Cell Signaling Technology Cat# 4511, RRID:AB_2139682), anti-SAPK/JNK (Cell Signaling Technology Cat# 9252, RRID:AB_2250373), anti-phospho-SAPK/JNK (T183/Y185) (Cell Signaling Technology Cat# 4668 P, RRID:AB_10831195), anti-MYC (Cell Signaling Technology Cat# 13987, RRID:AB_2631168), anti-MIZ1 (14300, Cell Signalling), anti-HBD1 (Abcam Cat# ab14425, RRID:AB_301206), anti-actin (Sigma-Aldrich Cat# A2066, RRID:AB_476693), anti-mouse IgG-POX (GE Healthcare Cat# NXA931-1ml, RRID:AB_772209), and anti-rabbit IgG-POX (GAR/IgG(H + L)/PO, Nordic Immunology).
Anti phospho p38 t180 y182
Manufactured by Cell Signaling Technology
Sourced in United States
Anti-phospho-p38 (T180/Y182) is a reagent that specifically detects phosphorylation of p38 MAPK at threonine 180 and tyrosine 182. This antibody can be used to monitor the activation state of the p38 MAPK signaling pathway.
Automatically generated - may contain errors
Lab products found in correlation
Anti phospho erk1 2 t202 y204, by Cell Signaling Technology (1 mentions)
Anti sapk jnk, by Cell Signaling Technology (1 mentions)
Anti actin, by Merck Group (1 mentions)
Cetuximab, by Creative Diagnostics (1 mentions)
Anti erk1 2, by Cell Signaling Technology (1 mentions)
Anti p38, by Cell Signaling Technology (1 mentions)
Anti phospho sapk jnk t183 y185, by Cell Signaling Technology (1 mentions)
Anti myc, by Cell Signaling Technology (1 mentions)
Anti ly6c pe, by BioLegend (1 mentions)
Cpg oligonucleotide, by InvivoGen (1 mentions)
Adenosine triphosphate (atp), by Merck Group (1 mentions)
X tremegene hp dna transfection reagent, by Roche (1 mentions)
Ultrapure lps, by InvivoGen (1 mentions)
Flagellin, by InvivoGen (1 mentions)
Nigericin, by InvivoGen (1 mentions)
Trizol reagent 15596018, by Thermo Fisher Scientific (1 mentions)
Bapta am b1205, by Thermo Fisher Scientific (1 mentions)
Anti chmp4b 13683 1 ap, by Proteintech (1 mentions)
Anti β actin sc 1615, by Santa Cruz Biotechnology (1 mentions)
Anti caspase 1 ag 20b 0042, by Adipogen (1 mentions)
5 protocols using anti phospho p38 t180 y182
1
Antibody Characterization for Cellular Signaling
2
Immune Cell Activation Assay
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Ultrapure LPS, flagellin, nigericin, CpG oligonucleotide, and poly(dA:dT) were purchased from InvivoGen. Silica (MIN-U-SIL 15) was obtained from US Silica. ATP was from Sigma-Aldrich. Pam3Cys was from EMC Microcollections. X-tremeGENE HP DNA transfection reagent was from Roche. Transfection reagent Profect P1 was from Targeting Systems. Acridine Orange (L13159) was from Alfa Aesar. TRIzol reagent (15596018), BAPTA-AM (B1205), and Alexa Fluor 594-labeled zymosan particles (Z23374) were from Thermo Fisher Scientific. Antibodies for immunoblotting include anti-CHMP4B (13683-1-AP) from Proteintech; anti-IKKα (05-536) from Millipore; anti-Mcu (14997), anti-phospho-IkBα (S32), anti-phospho-IKKα/β (S176/180), anti-phospho-p65(S536), anti-p65, anti-phospho-ERK1/2 (T202/Y204), anti-phospho-JNK (T183/Y185), and anti-phospho-p38 (T180/Y182) from Cell Signaling Technology; anti-β-actin (sc-1615) from Santa Cruz Biotechnology; anti-NLRP3 (Cryo-2), anti-Asc (AL177), and anti-caspase-1 (AG-20B-0042) from Adipogen; and anti-IL-1β (AF-401-NA) from R&D Systems. Antibodies for the flow cytometry assay include anti-CD45-PE Cy7 (103114), anti-CD11b-FITC (101206), anti-Ly-6G-PB (127612), and anti-Ly-6C-PE (128008) from BioLegend.
3
Signaling Pathway Characterization Protocol
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Anti–TAK‐1 (catalog no. D94D7), anti–MKK‐4 (catalog no. 9152S), anti–phospho–MKK‐4T261 (catalog no. 9151S), anti‐IκB (catalog no. 4814S), anti–phospho‐IκB, Ser32/36 (catalog no. 9246), anti–phospho–p38‐T180/Y182 (catalog no. 9211S), anti–phospho–TAK‐1‐T184/187 (catalog no. 45085), anti–phospho–IKKα/β‐Ser176/180 (catalog no. 16A6), and anti–phospho–activating transcription factor 2 (ATF‐2)–Thr71 (catalog no. 9221) were from Cell Signaling Technology. Anti–phospho–JNK‐pTpY183/185 (catalog no. 44682G) was obtained from Invitrogen. Anti–T‐ERK (catalog no. sc‐94), anti–NF‐κB–p65 (catalog no. Sc‐372), Anti–TAK‐1 (catalog no. sc‐7162), and anti–phospho–TAK‐1‐S192 (catalog no. sc‐130219) were from Santa Cruz Biotechnology. Anti‐ubiquitin–Lys‐63–specific antibody (catalog no. 05‐1308) was from Millipore. Myelin basic protein (MBP; catalog no. M1891) and anti–phospho‐ERK (catalog no. M9692) were from Sigma. A ubiquitin chain restriction enzyme analysis (UbiCREST) kit (catalog no. K‐400) was obtained from R&D Systems, and γ32P‐ATP was obtained from PerkinElmer. TAK‐1 inhibitor 5z‐7‐oxozeanol (catalog no. 3604) was from Tocris.
4
Antibodies Used for Western Blotting
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The following antibodies were used in this study: anti-VCAM-1 (#13662S), anti-ICAM-1 (#4915S), anti-phospho-p38 T180/Y182 (#4511) and anti-p38 MAPK (#8690) antibodies (Cell Signaling Technology), anti-β-actin (Sigma-Aldrich, #5316), anti-GRK2/3 (Millipore, #05465), anti-GRK4-6 (Millipore, #05466) and anti-GAPDH (GeneTex, #GT239). Secondary antibodies were anti-mouse or anti-rabbit HRP conjugated antibodies (Bio-Rad, #170–6516 and #170–6515).
5
Signaling Pathway Activation Profiling
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We used a nuclear extract and cytosol preparation kit (Apro Science, Naruto, Tokushima, Japan) following the manufacturer’s protocol. An appropriate amount of 1× phosphatase inhibitor cocktail (Roche, Indianapolis, IN) and 1× protease inhibitor cocktail (Roche)) was added and the protein concentration was measured by a Pierce protein assay kit (Thermo Fisher, Waltham, MA, USA). Aliquots of supernatants containing 20–30 μg of protein were separated and immunoblotted. Image was captured by WSE-6300H-CS LuminoGraph Ⅲ (ATTO, Tokyo, Japan). Total cellular extracts were prepared and immunoblotted as described. Signaling pathway molecules were examined using anti-phospho-p44/42 MAPK ERK1/2(Thr202/Tyr204), anti-phospho p38(T180/Y182), and p44/42 MAPK mouse monoclonal antibodies (all from Cell Signaling Technology, Beverly, MA, USA). Anti-p38α/stress-activated protein kinase (SAPK)2α mouse monoclonal antibody was obtained from BD Biosciences. Rabbit monoclonal β−actin (Cell Signaling Technology) was employed to access the equal loading of the protein.
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