13c12 bpa

Manufactured by Cambridge Isotopes

Sourced in United States

13C12-BPA is a stable isotope-labeled compound used as an internal standard in analytical measurements. It contains 12 carbon atoms labeled with the carbon-13 isotope. This compound is utilized in analytical techniques that require precise quantification and identification of target analytes.

Automatically generated - may contain errors

Lab products found in correlation

β glucuronidase, by Merck Group (2 mentions) Methanol, by Thermo Fisher Scientific (2 mentions) Optima lc ms grade methanol, by Thermo Fisher Scientific (1 mentions) D6 bpa, by CDN Isotopes (1 mentions) Creatinine, by Merck Group (1 mentions) Equol, by Merck Group (1 mentions) Enterolactone, by Merck Group (1 mentions) Enterodiol, by Merck Group (1 mentions) Sulfatase, by Merck Group (1 mentions) Genistein, by Merck Group (1 mentions) Daidzein, by Merck Group (1 mentions) Ethyl acetate, by Thermo Fisher Scientific (1 mentions) Acetonitrile, by Thermo Fisher Scientific (1 mentions) Formic acid, by Thermo Fisher Scientific (1 mentions) Ammonium acetate, by Thermo Fisher Scientific (1 mentions) Acetic acid lc ms grade, by Thermo Fisher Scientific (1 mentions) Tbbpa, by Wellington Laboratories (1 mentions) 13c12 tbbpa, by Cambridge Isotopes (1 mentions) Analytical grade formic acid, by Merck Group (1 mentions) Hplc grade methanol, by CNW Technologies (1 mentions)

9 protocols using 13c12 bpa

Quantifying Bisphenol Compounds and Metabolites

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BPA, BPS, BPF, BPAF, 4-methylumbelliferyl glucuronide, 4-methylumbelliferyl sulfate, ammonium acetate (>98%), and β-glucuronidase/sulfatase (Helix pomatia, H1) were purchased from Sigma-Aldrich Laboratories, Inc. (St. Louis, MO, USA). 13C₁₂–BPA and ¹³C₄-4-methylumbelliferone were obtained from Cambridge Isotope Laboratories, Inc. (Andover, MA, USA), and ¹³C₁₂–BPS and 13C₁₂–BPF were purchased from CanSyn Chem Corp. (Toronto, Canada). We used ¹³C₁₂–BPA as the internal standard for BPAF. Optima LC/MS grade methanol was purchased from Fisher Scientific (Pittsburgh, PA, USA). HPLC-grade formic acid (98%) was purchased from EM Science (Gibbstown, NJ, USA).

Ye X., Wong L.Y., Kramer J., Zhou X., Jia T, & Calafat A.M. (2015). Urinary Concentrations of Bisphenol A and Three Other Bisphenols in Convenience Samples of U.S. Adults during 2000–2014. Environmental science & technology, 49(19), 11834-11839.

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Bisphenol A Quantification Protocol

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Native BPA, β-glucuronidase/arylsulfatase (Helix pomatia, H1, 16 units/mg), unlabeled BPA-sulfate (BPA-S), and all other chemical reagents were purchased from Sigma Aldrich (St. Louis, MO) unless stated otherwise. ¹³C₁₂-BPA (>99% isotopic purity) was obtained from Cambridge Isotope Labs (Andover, MA), d6-BPA (99.5 atom %) from CDN Isotopes (Pointe-Claire, Quebec), unlabeled BPA-glucuronide (BPA-G; >99% isotopic purity) from Toronto Research Chemicals (North York, Ontario, Canada), and ¹³C₁₂-BPA-G (>99% isotopic purity) was synthesized by MRIGlobal (Kansan City, MO).

Thayer K.A., Doerge D.R., Hunt D., Schurman S.H., Twaddle N.C., Churchwell M.I., Garantziotis S., Kissling G.E., Easterling M.R., Bucher J.R, & Birnbaum L.S. (2015). Pharmacokinetics of Bisphenol A in Humans Following a Single Oral Administration. Environment international, 83, 107-115.

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Chemical Standards for Analytical Assays

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Analytical standards of BPA (≥97%), enterolactone (95%), enterodiol (95%), daidzein (98%), equol (99%), genistein (98%), creatinine (99%) and β-glucuronidase (145700 units/mL β-glucuronidase and 887 units/mL sulfatase) were purchased from Sigma-Aldrich (St. Louis, MO, USA). O-DMA (98%) was purchased from Plantech (Reading, UK). Stable isotope-labeled standards, ¹³C₁₂-BPA, D₃-daidzein and D₄-genistein were purchased from Cambridge Isotope Laboratories (Andover, MA, USA). D₃-creatinine (99%) was purchased from CDN Isotopes (Pointe-Claire, QC, Canada).

Zhang M., Duan Z., Wu Y., Liu Z., Li K, & Wang L. (2015). Occurrence and Profiles of the Artificial Endocrine Disruptor Bisphenol A and Natural Endocrine Disruptor Phytoestrogens in Urine from Children in China. International Journal of Environmental Research and Public Health, 12(12), 15110-15117.

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Deuterated BPA Quantification

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Deuterated d6-BPA (dimethyl-d6; 98% isotopic purity and 99% chemical purity; product # 588806-1G) was purchased from Sigma Aldrich (St. Louis, MO) and the corresponding labeled internal standard ¹³C₁₂-BPA (99%) was obtained from Cambridge Isotope Laboratories, Inc. (Andover, MA). LC/MS grade acetic acid (≥99.7%), acetonitrile (≥99.9%), ammonium acetate (≥99.0%), ethyl acetate (≥99.9%), formic acid (≥ 99.0%), methanol (≥ 99.9%), and water were purchased from Fisher Scientific (Hampton, NH). β-Glucuronidase/arylsulfatase enzyme from Helix pomatia was purchased from Sigma-Aldrich (BGALA-RO, product # 10127698001, Roche Life Science through Sigma Aldrich). All solvents, reagents, and experimental labware were tested for d6-BPA and ¹³C₁₂-BPA, and none was detected.

Sasso A.F., Pirow R., Andra S.S., Church R., Nachman R.M., Linke S., Kapraun D.F., Schurman S.H., Arora M., Thayer K.A., Bucher J.R, & Birnbaum L.S. (2020). Pharmacokinetics of bisphenol A in humans following dermal administration. Environment international, 144, 106031.

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Quantification of Terephthalic Acid and Bisphenol A

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PET (3-5 mm) and PC (3 mm) particles were purchased from Goodfellow Cambridge Ltd (Huntingdon, England). 1-Pentanol was purchased from Fisher Scientific (Pittsburgh, PA, USA). Terephthalic acid (TPA; >99% purity) was purchased from Toronto Research Chemicals Inc (North York, ON, Canada). D₄-TPA (99%) and BPA (>99%) were purchased from Sigma-Aldrich (St. Louis, MO, USA). ¹³C₁₂-BPA (99%) was purchased from Cambridge Isotope Laboratories (Andover, MA, USA). High-performance liquid chromatography (HPLC) grade methanol and water were supplied by J.T. Baker (Phillipsburg, NJ, USA). Solid-phase extraction (SPE) cartridges (HLB, 200 mg/6 cc) were purchased from Waters (Milford, MA, USA).

Zhang J., Wang L, & Kannan K. (2021). Quantitative analysis of polyethylene terephthalate and polycarbonate microplastics in freshwater and coastal sediment collected from South Korea, Japan and the USA. Chemosphere, 279, 130551.

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TBBPA Degradation Study Analytical Methods

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Tetrabromobisphenol A (TBBPA, 4,4′-Isopropylidenebis (2,6-dibromophenol), 97% purity, CAS 79-94-7) used in the degradation study was purchased from Sigma-Aldrich (St Louis, MO, US). TBBPA stock solutions (0.4 g/l) were prepared in high performance liquid chromatography (HPLC) grade acetone. For LC-MS/MS analysis, TBBPA for calibration standards was purchased from Wellington Laboratories (Guelph, Canada). Bisphenol A (BPA; CAS 80-05-7) used for LC-MS/MS analysis was purchased from AccuStandard (New Haven, CT, US). Mono, di and tri-bromobisphenol A (CAS 6073-11-6, 29426–78–6, and 6386-73-8, respectively) used for LC-MS/MS analysis was provided by Dr. Göran Marsh, (Stockholm University, SE). ¹³C₁₂-tetrabromobisphenol A (Wellington Laboratories, Guelph, Canada) served as the internal standard used for TBBPA and lesser brominated BPAs. ¹³C₁₂-BPA (Wellington Laboratories, Guelph, Canada) served as the internal standard for BPA. D₈-BPA (Wellington Laboratories, Guelph, Canada) and ¹³C₁₂- 6-hydroxy-2,2',4,4'-tetrabromodiphenyl ether (Cambridge Isotope Laboratories, Tewksbury, MA, US) were used as recovery standards to assess recoveries of ¹³C₁₂-BPA and ¹³C₁₂-TBBPA, respectively. Solvents used for LC-MS/MS analyses were purchased from Honeywell, Burdick & Jackson Laboratories (St. Muskegon, MI, US).

Lefevre E., Cooper E., Stapleton H.M, & Gunsch C.K. (2016). Characterization and Adaptation of Anaerobic Sludge Microbial Communities Exposed to Tetrabromobisphenol A. PLoS ONE, 11(7), e0157622.

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Bisphenol Exposure Analysis Protocol

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Bisphenols used included BPA (purity ≥ 99%, Cat#: 239658; Lot#: MKBQ5209V; Aldrich Chemical Co., Milwaukee, WI, USA), BPS (4,4’-sulfonyldiphenol, purity 99.7%, Cat#: 146915000, Lot#: A0337011, Acros Organics, Geel, Belgium), and BPF (purity 98%, Cat#: B47006; Lot#: 05712ME; Sigma-Aldrich, St. Louis, MO, USA) dissolved in corn oil. The internal standards ¹³C₁₂-BPA (99%), ¹³C₁₂-BPS (98%) and ¹³C₁₂-BPF (99%) were purchased from Cambridge Isotope Laboratories (Andover, MA, USA). β-glucuronidase (≥100,000 units/mL β-glucuronidase; ≤ 7,500 units/mL sulfatase) from Helix pomatia (Type HP-2) was purchased from Sigma-Aldrich (St. Louis, MO, USA)

Gingrich J., Pu Y., Ehrhardt R., Karthikraj R., Kannan K, & Veiga-Lopez A. (2018). Toxicokinetics of bisphenol A, bisphenol S, and bisphenol F in a pregnancy sheep model. Chemosphere, 220, 185-194.

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Quantification of Bisphenol Analogs in Cells

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BPA- ({diphenyl-}^{13} C_{12})

(

{BPA-}^{13} C_{12}

, 98%;

100 μ g / mL

acetonitrile) and

BPS- ({diphenyl-}^{13} C_{12})

(

{BPS-}^{13} C_{12}

, 98%;

100 μ g / mL

methanol) were from Cambridge Isotope Laboratories. BPS (98%, solid) and Lucifer Yellow dilithium salt were from Sigma-Aldrich.

β -glucuronidase

and sulfatase from Helix pomatia (

\geq 100,000 units / mL

β -glucuronidase

;

\leq 7,500 units / mL

sulfatase) were from Sigma-Aldrich. A lactate dehydrogenase (LDH) cytotoxicity kit was from TaKaRa. TRITON® X-100, PROTEIN GRADE® Detergent (10% solution, sterile filtered) was from Sigma-Aldrich. Liquid chromatography (LC)–grade water, methanol, and acetonitrile were from Fisher Scientific. LC-grade ammonium acetate and analytical grade formic acid (98%) were from Sigma-Aldrich.

Liu J, & Martin J.W. (2019). Comparison of Bisphenol A and Bisphenol S Percutaneous Absorption and Biotransformation. Environmental Health Perspectives, 127(6), 067008.

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BPA Quantification by LC-MS/MS

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The BPA standard was purchased from Aldrich-Sigma (purity >98%, St. Louis, MO, USA). BPA-13 C12 (Cambridge Isotope Laboratories Ins, USA), HPLC-grade methanol, ethyl acetate and ammonium acetate (CNW Technologies) were purchased from Shanghai ANPEL Scientific Instrument Co., Ltd. β-Glucuronidase from Helix pomatia (Type HP-2, aqueous solution, β-glucuronidase activity ≥100000 units/mL, sulfatase activity ≤7500 units/mL) was purchased from Sigma-Aldrich. Deionized water for the preparation of buffers was purified by a Milli-Q ® water-purification system (Millipore, Bedford, MA, USA). An Oasis HLB cartridge (Waters, 200 mg/6 cm 3 , Milford, MA, USA) was purchased from Waters.
Stock solutions of BPA and 13 C12-BPA were prepared at a concentration of 1 mg/mL in methanol. All stock solutions were stored at -20°C. Calibration standards, ranging from 0.20 to 25 μg/L, were prepared from the stock solution through serial dilution by using a methanol-water mixture (50/50, v/v); isotope stock solutions ( 13 C12-BPA, 100 μg/L) were also prepared by using the same methanol-water mixture.

Wang H., Liu L., Eqani S.A.M.A.S, & Shen H. (2017). Infant Exposure to Bisphenol A Can Be Quantitatively Assessed by a Simply Improved High-Performance Liquid Chromatography-Tandem Mass-Spectrometry Method. Analytical sciences : the international journal of the Japan Society for Analytical Chemistry, 33(7).

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