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Pierce elisa mouse mab isotyping kit

Manufactured by Thermo Fisher Scientific
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The Pierce ELISA Mouse mAb Isotyping Kit is a laboratory product designed to identify the isotype of mouse monoclonal antibodies (mAbs). It provides a reliable and efficient way to determine the immunoglobulin class and subclass of mouse mAbs generated from hybridoma cell lines or other sources.

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Lab products found in correlation

Easysep mouse b cell enrichment kit, by STEMCELL (2 mentions) Anti mouse cd40, by BD (2 mentions) Lipopolysaccharides (lps), by Merck Group (2 mentions) Cell proliferation kit 1, by Roche (2 mentions)

Close spelling variants of this product

Mouse ELISA kits (105 citations) ELISAs (35 citations) Pierce Rapid ELISA Mouse mAb Isotyping Kit (5 citations)

2 protocols using pierce elisa mouse mab isotyping kit

1

Naive B Cell Activation and Proliferation

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Naïve B cells from splenic cell suspensions were negatively selected using EasySep Mouse B cell Enrichment Kit, following the manufacturer’s instructions (StemCell). Purified B cells were suspended in RPMI containing 10% FBS, L-glutamine, and 50μM β-mercaptoethanol (complete medium). For immunoglobulin (Ig) synthesis, naïve B cells (106 cell per ml) were cultures in complete medium alone, with LPS (20μg per ml; Sigma-Aldrich) or anti-mouse CD40 (100ng per ml; Pharmingen). Supernatants were collected after 6 days and analyzed for various Ig production by Pierce ELISA Mouse mAb Isotyping Kit, following the manufacturer’s instructions (Thermo Scientific). Proliferation was measured using Cell Proliferation Kit I (MTT), following the manufacturer’s instructions (Roche). For proliferation, aliquots of 105 B cell in 100μl of complete medium alone, or in presence LPS (20μg per ml) or anti-mouse CD40 (100ng per ml) were cultured in a 96-well flat-bottom plate for 48 hours, then the MTT labeling reagent was added to a final concentration 0.5mg per ml following by overnight incubation with the solubilization solution. Proliferation was assessed by measuring the absorbance using a microplate (ELISA) reader.
Li J., Jørgensen S.F., Maggadottir S.M., Bakay M., Warnatz K., Glessner J., Pandey R., Salzer U., Schmidt R.E., Perez E., Resnick E., Goldacker S., Buchta M., Witte T., Padyukov L., Videm V., Folseraas T., Atschekzei F., Elder J.T., Nair R.P., Winkelmann J., Gieger C., Nöthen M.M., Büning C., Brand S., Sullivan K.E., Orange J.S., Fevang B., Schreiber S., Lieb W., Aukrust P., Chapel H., Cunningham-Rundles C., Franke A., Karlsen T.H., Grimbacher B., Hakonarson H., Hammarström L, & Ellinghaus E. (2015). Association of CLEC16A with human common variable immunodeficiency disorder and role in murine B cells. Nature communications, 6, 6804.
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2

Naive B Cell Activation and Proliferation

Check if the same lab product or an alternative is used in the 5 most similar protocols
Naïve B cells from splenic cell suspensions were negatively selected using EasySep Mouse B cell Enrichment Kit, following the manufacturer’s instructions (StemCell). Purified B cells were suspended in RPMI containing 10% FBS, L-glutamine, and 50μM β-mercaptoethanol (complete medium). For immunoglobulin (Ig) synthesis, naïve B cells (106 cell per ml) were cultures in complete medium alone, with LPS (20μg per ml; Sigma-Aldrich) or anti-mouse CD40 (100ng per ml; Pharmingen). Supernatants were collected after 6 days and analyzed for various Ig production by Pierce ELISA Mouse mAb Isotyping Kit, following the manufacturer’s instructions (Thermo Scientific). Proliferation was measured using Cell Proliferation Kit I (MTT), following the manufacturer’s instructions (Roche). For proliferation, aliquots of 105 B cell in 100μl of complete medium alone, or in presence LPS (20μg per ml) or anti-mouse CD40 (100ng per ml) were cultured in a 96-well flat-bottom plate for 48 hours, then the MTT labeling reagent was added to a final concentration 0.5mg per ml following by overnight incubation with the solubilization solution. Proliferation was assessed by measuring the absorbance using a microplate (ELISA) reader.
Li J., Jørgensen S.F., Maggadottir S.M., Bakay M., Warnatz K., Glessner J., Pandey R., Salzer U., Schmidt R.E., Perez E., Resnick E., Goldacker S., Buchta M., Witte T., Padyukov L., Videm V., Folseraas T., Atschekzei F., Elder J.T., Nair R.P., Winkelmann J., Gieger C., Nöthen M.M., Büning C., Brand S., Sullivan K.E., Orange J.S., Fevang B., Schreiber S., Lieb W., Aukrust P., Chapel H., Cunningham-Rundles C., Franke A., Karlsen T.H., Grimbacher B., Hakonarson H., Hammarström L, & Ellinghaus E. (2015). Association of CLEC16A with human common variable immunodeficiency disorder and role in murine B cells. Nature communications, 6, 6804.
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