H 7700

Ten ml of blood samples were collected one day before of beginning of exercise and one day after the day of final exercise. Venous blood samples were collected after 4 h of fasting. Serum for intervention was collected within 10 min and serum for cross-sectional analysis was collected after standing for 1 h to coagulate the blood. Sera were preserved at −80°C until analysis. Serum LDL-cholesterol was measured using automated XE-2100 (Sysmex, Kobe, Japan) and H7700 (Hitachi High-Technologies, Tokyo, Japan), and high-sensitivity CRP (hs-CRP) was measured by a highly sensitive CRP assay (Behring Latex-Enhanced using the Behring Nephelometer BN-100; Behring Diagnostics, Westwood, MA). Arginase activity and IgE were determined using ELISA kits (Montgomery, TX), and arginase I was determined with ELISA kits previously established.^{(31 (link))} Information on lifestyle factors, including cigarette smoking, exercise habits, and alcohol drinking habits, was obtained using self-reported questionnaires or clinical records.

The bladder specimens were quickly sliced into 1 mm³ and immersed in 2.5% glutaraldehyde solution for 48 h. Subsequently, postfixation was performed with 1% osmium tetroxide for 2 h, and dehydration followed by impregnation with epoxy resin was performed. The ultrathin sections were made at 70~80nm and stained with 2% uranyl acetate and Reynolds lead citrate solution. The sections were observed under a transmission electron microscope (H-7700; Hitachi High-Technologies, Tokyo, Japan).

TPA-treated SPEL cells were pelleted by centrifugation, fixed with 2.5 % glutaraldehyde and 2 % paraformaldehyde in 0.1 M phosphate buffer (pH 7.4) for 2 h at room temperature, post-fixed in 1 % osmium tetroxide and embedded in Epon. Ultrathin sections were stained with uranyl acetate and lead citrate, and observed under a transmission electron microscope (H7700, Hitachi High Technologies, Tokyo, Japan) at 80 kV.