Fluorescent staining for flow cytometric analysis was performed in M1 or M2 in FACS buffer (PBS with 1% bovine serum albumin and 0.1% sodium azide). Fc receptor-mediated non-specific antibody binding was blocked by using human TruStain FcX solution (Biolegend, San Diego, CA). Macrophages were stained for 30 min at 4 °C. The following antibodies were used: anti-human APC CD54 (HA58; Cat. no. 559771; 15 µl/106 cells in 100 µl), anti-human FITC CD206 (19.2; Cat. no. 551135; 10 µl/106 cells in 100 µl) (BD Bioscience, San Jose, CA), anti-human PE CD80 (2D10; Cat. no. 305208; 10 µl/106 cells in 100 µl), and anti-human PerCP/Cy5.5 CD163 (RM3/1; Cat. no. 326512; 15 µl/106 cells in 100 µl) (Biolegend, San Diego, CA). For surface expression of DRV1 and BLT1, polarized M1 and M2 were stained with PE anti-human BLT1 (203/14F11; Cat. no. 552836; 15 µl/106 cells in 100 µl; BD Bioscience) or rabbit anti-human DRV1 (GPR32) antibody (Cat. no. GTX108119; 2 µl/106 cells in 100 µl; GeneTex, Irvine, CA), followed by non-immune rabbit IgG for 30 min. Macrophages (M1 or M2) were analyzed using FACSCanto II (BD Bioscience) flow cytometer, and data analyzed using FlowJo X Software.
Anti human fitc cd206 19.2
Manufactured by BD
The Anti-human FITC CD206 (19.2) is a fluorescently-labeled antibody that specifically binds to the CD206 antigen expressed on human cells. CD206, also known as the macrophage mannose receptor, is a cell surface receptor involved in the endocytosis of glycoproteins. This antibody can be used to detect and identify CD206-positive cells in various applications.
Automatically generated - may contain errors
Lab products found in correlation
Facscanto 2, by BD (2 mentions)
Human trustain fcx solution, by BioLegend (2 mentions)
Anti human apc cd54 ha58, by BD (2 mentions)
Anti human pe cd80 2d10, by BioLegend (2 mentions)
Anti human percp cy5.5 cd163 rm3 1, by BioLegend (2 mentions)
Pe anti human blt1 203 14f11, by BD (2 mentions)
Rabbit anti human drv1 gpr32 antibody, by BD (2 mentions)
2 protocols using anti human fitc cd206 19.2
1
Macrophage Phenotyping by Flow Cytometry
2
Macrophage Phenotyping by Flow Cytometry
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Fluorescent staining for flow cytometric analysis was performed in M1 or M2 in FACS buffer (PBS with 1% bovine serum albumin and 0.1% sodium azide). Fc receptor-mediated non-specific antibody binding was blocked by using human TruStain FcX solution (Biolegend, San Diego, CA). Macrophages were stained for 30 min at 4 °C. The following antibodies were used: anti-human APC CD54 (HA58; Cat. no. 559771; 15 µl/106 cells in 100 µl), anti-human FITC CD206 (19.2; Cat. no. 551135; 10 µl/106 cells in 100 µl) (BD Bioscience, San Jose, CA), anti-human PE CD80 (2D10; Cat. no. 305208; 10 µl/106 cells in 100 µl), and anti-human PerCP/Cy5.5 CD163 (RM3/1; Cat. no. 326512; 15 µl/106 cells in 100 µl) (Biolegend, San Diego, CA). For surface expression of DRV1 and BLT1, polarized M1 and M2 were stained with PE anti-human BLT1 (203/14F11; Cat. no. 552836; 15 µl/106 cells in 100 µl; BD Bioscience) or rabbit anti-human DRV1 (GPR32) antibody (Cat. no. GTX108119; 2 µl/106 cells in 100 µl; GeneTex, Irvine, CA), followed by non-immune rabbit IgG for 30 min. Macrophages (M1 or M2) were analyzed using FACSCanto II (BD Bioscience) flow cytometer, and data analyzed using FlowJo X Software.
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