The metabolite sample analysis was performed in Shimadzu GC–MS-QP2010™ coupled with an autosampler-auto injector (AOC-20si) and an SH-Rxi.5Sil MS capillary column (30 m x 0.25 µm film thickness x 0.25 µm internal diameter; Restek Corporation, USA). A derivatized sample of 2 µL volume was injected into the inlet at 250 °C with a split ratio of 1:5. Purging of the standard septum was performed after sample injection at 3 mL min−1 and carrier gas helium at 1 mL min−1 attained a constant flow rate. Initially, the oven temperature was programmed at 80 °C for 2 min, ramped up to 250 °C at 5 °C min−1, withheld for 2 min and further ramped to 300 °C at 10 °C min−1, and withheld for 24 min. Ionization was done at 70 eV in an electron impact mode and the masses were scanned for full spectra from 40 to 600 m/z with a scan speed of 2000. The solvent cut time was at 4 min.
Sh rxi 5sil ms capillary column
Manufactured by Restek
Sourced in United States
The SH-Rxi.5Sil MS capillary column is a fused silica column designed for gas chromatography-mass spectrometry (GC-MS) analysis. It features a 0.5 μm film thickness of a proprietary stationary phase.
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Lab products found in correlation
2 protocols using sh rxi 5sil ms capillary column
1
GC-MS Analysis of Metabolite Samples
2
GC-MS Untargeted Metabolite Analysis
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Following derivatization, the injection was set at Split Mode, with a split ratio of 5 and 0.2 μL injection volumes. The GC–MS analysis for untargeted metabolites consisted of a Shimadzu Gas chromatogram (GC-2010 plus) coupled with a mass spectrometer (TQ 8050), and an auto-sampler (AOC-20s) and auto-injector (AOC-20i) was used. For analysis, helium carrier gas (flow rate 1 mL min−1) and SH-Rxi-5Sil MS capillary column (30 m × 0.25 μm, 0.25 mm) (Restek Corporation, Bellefonte, PA, USA) were used. The system was set at 80 °C isothermal heating (2 min), followed by a ramp rate of 5 °C min−1 to 250 °C, 2 min withhold, and a 10 °C min−1 final ramp with 24 min withhold time. The total run time for GC–MS was 68 min with 4.5 min of solvent delay [27 (link)]. The GC–MS solution software Version 4.45 SP 1 was applied for chromatogram integration and analysis of mass spectra. The derivatized metabolites were identified using NIST14s and the WILEY8 spectral library.
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