Isolated exosomes were fixed by addition of 4% paraformaldehyde, 5 μl of the sample was dropped on a carbon coated 400 mesh Cu/Rh grid (Ted Pella, Redding, CA, USA) and stained with 5 μl of 1% uranyl acetate (Polysciences, Warrington, PA, USA) prepared in filtered distilled water. The grids were imaged with a FEI Talos L120C TEM with Gatan 4k × 4 k OneView camera.
Carbon coated 400 mesh cu rh grid
Manufactured by Ted Pella
Sourced in United States
Carbon-coated 400 mesh Cu/Rh grids are a type of laboratory equipment used for sample preparation in electron microscopy. These grids consist of a copper and rhodium alloy mesh with a thin carbon film deposited on the surface. The primary function of these grids is to provide a stable and conductive support for thin samples during imaging in electron microscopes.
Automatically generated - may contain errors
Lab products found in correlation
Uranyl acetate, by Polysciences (4 mentions)
Talos l120c tem, by Thermo Fisher Scientific (2 mentions)
4k 4 k oneview camera, by Thermo Fisher Scientific (2 mentions)
Cm 12 transmission electron microscope, by Ametek (2 mentions)
Em gp, by Leica (2 mentions)
Tecnai 12, by Thermo Fisher Scientific (2 mentions)
Tecnai 20 feg electron microscope, by Thermo Fisher Scientific (2 mentions)
Gatan 4 k 2.7 k digital camera, by Ametek (1 mentions)
6 protocols using carbon coated 400 mesh cu rh grid
1
Exosome Imaging by Transmission Electron Microscopy
2
Transmission Electron Microscopy Imaging
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After complexation, 5 µL of each sample was dropped onto a carbon-coated 400 mesh Cu/Rh grid (Ted Pella, Redding, CA, USA) and stained with 5 µL of 1% uranyl acetate (Polysciences, Warrington, PA, USA) which was prepared in filtered distilled water. A FEI Talos L120C TEM with a Gatan 4 k × 4 k OneView camera was used to image the grids.
3
Oligomeric Aβ42 Visualization by Electron Microscopy
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The presence of oligomeric forms of Aβ42 in the culture supernatants was additionally assessed by electron microscopy using our previously described protocols [8 (link), 14 (link)]. Briefly, 3 μl aliquots were placed onto carbon-coated 400-mesh Cu/Rh grids (Ted Pella, Inc., Redding, CA, USA) and stained with 1% uranyl acetate in distilled water (Polysciences, Inc., Warrington, PA, USA). Stained grids were examined in a Philips CM-12 transmission electron microscope and photographed with a Gatan (4 k × 4 k) digital camera at the NYU School of Medicine Microscopy Laboratory Core Facility.
4
Dynamin Interactions with Liposomes Visualized by Electron Microscopy
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For negative stain EM, samples (1-3 μM dynamin and 200 μM DOPS liposomes incubated in the presence or absence of 1 mM GTP or GMPPCP for 30 min at room temperature) were absorbed onto carbon-coated 400 mesh Cu/Rh grids (Ted Pella, Inc.), stained with 2% Uranyl Acetate, and imaged in a Tecnai 12 (FEI) transmission electron microscope at 120kV using a 2 × 2 Gatan CCD camera. For cryo-EM, a 3.5 μl sample (prepared as described above) was placed on a plasma-cleaned (Fishione Inc.). Quantifoil holey carbon EM grid (SPI Supplies), blotted with filter paper, and flash-frozen in liquid ethane using a Leica EM GP (Leica Microsystems). The grids were subsequently stored in liquid nitrogen. The vitrified samples were imaged at liquid nitrogen temperature on a Tecnai 20 FEG electron microscope (FEI) operating at 200kV and images were collected with a 4 × 4 CCD camera.
5
Dynamin Interactions with Liposomes Visualized by Electron Microscopy
6
Electron Microscopy of Amyloid-Beta Peptide Oligomerization
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Peptide oligomerization was also monitored by electron microscopy, as previously described [38 (link), 66 (link), 71 (link)]. Five microliters of either monomeric or oligomeric preparations of the different Aβ peptides were placed onto carbon-coated 400 mesh Cu/Rh grids (Ted Pella, Inc., Redding, CA) and stained with 1% uranyl acetate in distilled water (Polysciences, Inc., Warrington, PA). The stained grids were examined in a Philips CM-12 transmission electron microscope and photographed with a Gatan (4 k × 2.7 k) digital camera at the Image Core Facility of the Skirball Institute of Biomedical Medicine, NYU School of Medicine, as described [66 (link), 71 (link), 72 (link)].
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