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Gsk2194069

Manufactured by Merck Group
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GSK2194069 is a laboratory equipment product. It is a device used for scientific research and analysis purposes. The core function of this product is to facilitate specific experimental or measurement tasks within a laboratory setting. No further details about the intended use or capabilities of this product can be provided in an unbiased and factual manner.

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Lab products found in correlation

Cellevent caspase 3 7 green detection reagent, by Thermo Fisher Scientific (3 mentions) Hoechst 33342, by Thermo Fisher Scientific (2 mentions) Tunicamycin, by Cayman Chemical (1 mentions) Bromoenol lactone, by Cayman Chemical (1 mentions) Nutlin 3, by MedChemExpress (1 mentions) Gsk2606414, by Cayman Chemical (1 mentions) Cerulenin, by Santa Cruz Biotechnology (1 mentions) Sb 204990, by Cayman Chemical (1 mentions) Torin2, by MedChemExpress (1 mentions) Cay10566, by Cayman Chemical (1 mentions) Dimethyl sulfoxide (dmso), by Merck Group (1 mentions) Luteolin, by Enzo Life Sciences (1 mentions) Staurosporin, by Merck Group (1 mentions) Phospho acc, by Cell Signaling Technology (1 mentions) Acsl1, by Cell Signaling Technology (1 mentions) Perchloric acid, by Merck Group (1 mentions) Sodium palmitate, by Merck Group (1 mentions) Pbabe nf2, by Addgene (1 mentions) 5 iodotubercidin, by Enzo Life Sciences (1 mentions) Anti gapdh antibody, by Merck Group (1 mentions)

3 protocols using gsk2194069

1

Lipid Metabolism Modulators Protocol

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Chemicals used were C75 (Sigma Aldrich), TOFA (5-(tetradecyloxy)-2-furoic acid), acetyl-CoA carboxylase inhibitor (Abcam, ab141578), Triascin C (Enzo, BML-EI218-100), Bromoenol Lactone (Cayman Chemical, 70700), Etomoxir (Calbiochem/MilliporeSigma), T-863 (Cayman Chemical, 25807), SB 204990 (Cayman Chemical, 15245), Cerulenin (Santa Cruz Biotechnology), GSK2194069 (Sigma Aldrich), CAY10566, GSK2606414 (both Cayman Chemical), Tunicamycin (Cayman Chemical), MMS (Santa Cruz Biotechnology), NCS (Sigma Aldrich), Torin2, Nutlin-3 (both Medchemexpress), DMSO (Sigma Aldrich). Concentrations used are noted in the Fig. legends and text.
Köberlin M.S., Fan Y., Liu C., Chung M., Pinto A.F., Jackson P.K., Saghatelian A, & Meyer T. (2024). A fast-acting lipid checkpoint in G1 prevents mitotic defects. Nature Communications, 15, 2441.
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2

Lipid Metabolism Regulation in NF2 Cells

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pBabe-NF2 was obtained from Addgene. Anti-Fasn siRNA (M-040091-01-0005), anti-Acaca siRNA (M-063938-01-0005), anti-Mtor (M-065427-00-0005), anti-Rptor (M-058754-01-0005), anti-Rictor (M-064598-01-0005) and non-silencing (D-001206-13-05) siRNA were purchased from Dharmacon. Individual siRNAs against Mtor (SASI_Mm01-00164496 and -00164492), Rictor (-00137732 and -00137730), Rptor (-00055298 and -00334580), Fasn (-00177858 and -00177854), Acaca (-0011590 and -00115905), and Mlycd (-00028572 and -00028576) were purchased from Sigma-Aldrich. Anti-Merlin antibodies were purchased from Abcam (#ab88957). Lipid synthesis and metabolism antibody kit (includes anti-Fasn, -phospho ACC, -ACC, -Lipin1, -ACLY, -phospho ACLY, -ACSL1, and -ACECS1 antibodies), and anti-Casp3 antibodies were purchased from Cell Signaling Technology. Anti-SREBP1 antibodies were purchased from Santa Cruz Biotechnology. Anti-GAPDH antibodies were purchased from EMD-Millipore.
Cerulenin, C75, luteolin, 5-(tetradecyloxy)-2-furoic acid (TOFA) and 5-iodotubercidin were purchased from Enzo Life Sciences. GSK2194069, dimethylsulfoxyde (DMSO), staurosporin, sodium palmitate, 70% perchloric acid, ammonium formate, acetonitrile, acetyl-coenzyme A lithium salt, malonyl coenzyme A lithium salt, propionyl-coenzyme A lithium salt, and poly-L-lysine were purchased from Sigma-Aldrich.
Stepanova D.S., Semenova G., Kuo Y.M., Andrews A.J., Ammoun S., Hanemann C.O, & Chernoff J. (2017). An essential role for the tumor suppressor Merlin in regulating fatty acid synthesis. Cancer research, 77(18), 5026-5038.
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3

Lipid Metabolism and Cell Signaling

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DHA, AA, OA, PA, betulin, GSK2194069, PF-429242, fluorescein diacetate, and 1,10 phenanthroline were obtained from Sigma Aldrich. C-Laurdan was purchased from TPProbes (South Korea). Amplex Red kit to quantify cholesterol was purchased from Invitrogen. Antibodies used: “actin (1:5000, monoclonal clone AC-15, Abcam), SREBP2 (1:400, polyclonal, Abcam), SREBP1 (1:1000, monoclonal, Abcam), ATF6 mentioned (1:1000, polyclonal, Bethyl Laboratories). Di-4-ANEPPDHQ (Di4), CellEvent™ Caspase-3/7 Green Detection Reagent, Annexin V-Pacific Blue, and Hoechst 33342 purchased from ThermoFisher. Fluorescein sodium salt was purchased from Santa Cruz Biotechnology.
Levental K.R., Malmberg E., Symons J.L., Fan Y.Y., Chapkin R.S., Ernst R, & Levental I. (2020). Lipidomic and biophysical homeostasis of mammalian membranes counteracts dietary lipid perturbations to maintain cellular fitness. Nature Communications, 11, 1339.
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