Gemini field emission gun sem300

Manufactured by Zeiss

Sourced in United States

The ZEISS Gemini field emission gun SEM300 is a scanning electron microscope that utilizes a field emission gun as its electron source. It is designed to provide high-resolution imaging of samples at the nanoscale level.

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Lab products found in correlation

3view2xp in situ ultramicrotome, by Ametek (1 mentions) Onpoint detector, by Ametek (1 mentions) 3view ultramicrotome, by Ametek (1 mentions) Onpoint, by Ametek (1 mentions)

Close spelling variants of this product

Field Emission Gun (4 citations) SEM300 (3 citations)

3 protocols using gemini field emission gun sem300

Serial Block Face Scanning Electron Microscopy

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SBF-SEM was performed with a ZEISS Gemini field emission gun SEM300 (Zeiss, Marly-le-Roi, France), equipped with a 3View2XP in situ ultramicrotome (Gatan Inc., Pleasanton, CA, USA). Serial section thickness was set to 30 nm. The block-face was imaged with the accelerating voltage set to 1.2 kV and backscattered electrons were detected with an OnPoint Detector (Gatan Inc.) and a pixel dwell time of 6 µs. Images were acquired in high vacuum mode with magnifications and image sizes adjusted to a final pixel size of 10 nm at specimen level.

Palhol J.S., Balia M., Sánchez-Román Terán F., Labarchède M., Gontier E, & Battefeld A. (2023). Direct association with the vascular basement membrane is a frequent feature of myelinating oligodendrocytes in the neocortex. Fluids and Barriers of the CNS, 20, 24.

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Transmission Electron Microscopy Imaging

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The sample on the pin was placed into the carrier that fits into the3View ultramicrotome (3viewXP2- Gatan Inc., Pleasanton, CA, USA) on a ZEISS Gemini field emission gun SEM300 (Zeiss - Marly-le-Roi - France). The block face was imaged with an accelerating voltage at 1.2 kV using the Back scattered electron with a specific BSE Detector (On point - Gatan Inc., Pleasanton, CA, USA).

de Senneville B.D., Khoubai F.Z., Bevilacqua M., Labedade A., Flosseau K., Chardot C., Branchereau S., Ripoche J., Cairo S., Gontier E, & Grosset C.F. (2021). Deciphering tumour tissue organization by 3D electron microscopy and machine learning. Communications Biology, 4, 1390.

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3View Ultramicrotome SEM Imaging

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The sample on the pin was placed into the carrier that fits into the3View ultramicrotome (3viewXP2-Gatan Inc., Pleasanton, CA, USA) on a ZEISS Gemini field emission gun SEM300 (Zeiss -Marly-le-Roi -France). The block face was imaged with an accelerating voltage at 1.2kV using the Back scattered electron with a specific BSE Detector (On point -Gatan Inc., Pleasanton, CA, USA).

Senneville B.D.d., Khoubai F.Z., Bevilacqua M., Labedade A., Flosseau K., Chardot C., Branchereau S., Ripoche J., Cairo S., Gontier É., & Grosset C. (2021). Deciphering Tumour Tissue Organization by 3D Electron Microscopy and machine learning.

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