Immunocytochemistry was also performed on ARPE-19 cells grown as monolayers on transwell plates, fixed in 4% paraformaldehyde. After extensive washing, cells were incubated either in rabbit polyclonal antibodies recognizing anti-HMGB1 (1:200; Invitrogen, Carlsbad, CA, USA) in blocking solution (0.5% Triton-X in tris buffered saline with 10% goat serum), and followed by Alexa Fluor 488 (1:500; Sigma Chemical Co., USA) as the secondary antibody. Staining was examined via fluorescence microscopy (Zeiss, USA) equipped with a digital camera.
Anti hmgb1
Manufactured by Thermo Fisher Scientific
Sourced in United States
The Anti-HMGB1 is a laboratory reagent designed for the detection and quantification of High Mobility Group Box 1 (HMGB1) protein. HMGB1 is a chromatin-binding nuclear protein involved in various cellular processes. The Anti-HMGB1 reagent can be used in immunoassay applications to measure HMGB1 levels in biological samples.
Automatically generated - may contain errors
Lab products found in correlation
3 protocols using anti hmgb1
1
Immunocytochemical Analysis of HMGB1 in ARPE-19 Cells
2
Necroptosis Pathway Protein Analysis
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Fisetin and z-VAD were purchased from Sigma, USA. The primary antibodies used in this study for Western blotting were as follows: anti-HMGB1 (Invitrogen, NY, USA), 1:1000 dilution; anti-ZBP1 (R&D system, USA), 1:1000 dilution; anti-RIP3 (Calbiochem, San Diego, CA), 1:1000 dilution; anti-RIP1 (Calbiochem, San Diego, CA), 1:1000 dilution; anti-MLKL (BD Biosciences, USA), 1:1000 dilution; and anti-GAPDH (Santa Cruz, CA, USA), 1:5000 dilution.
3
Targeted Cell Death Mechanism Analysis
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Fisetin and z-VAD were purchased from Sigma, USA. The primary antibodies used in this study for western blotting were as follows: anti-HMGB1(Invitrogen, NY, USA), 1:1000 dilution; anti-ZBP1 (R&D system, USA), 1:1000 dilution; anti-RIP3(Calbiochem, San Diego, CA), 1:1000 dilution; anti-RIP1(Calbiochem, San Diego, CA), 1:1000 dilution; anti-MLKL (BD Biosciences, USA), 1:1000 dilution; anti-GAPDH (Santa Cruz, CA, USA), 1:5000 dilution.
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