Aspergillopepsin I, equine apomyoglobin, tris(2-carboxyethyl)phosphine hydrochloride (TCEP), N-(2-aminoethyl)maleimide trifluoroacetate salt (NAEM), and sodium cyanoborohydride were purchased from Sigma-Aldrich. Twenty μm aldeyhye beads were purchased from POROS. One μm aldehyde/sulfate latex beads were purchased from Invitrogen. IdeS (FabRICATOR) was purchased from Genovis.
Equine apomyoglobin
Manufactured by Merck Group
Sourced in United States
Equine apomyoglobin is a laboratory product used for research and analysis purposes. It is a protein derived from horse muscle tissue. The core function of equine apomyoglobin is to serve as a standardized reference material for various biochemical and analytical procedures.
Automatically generated - may contain errors
Lab products found in correlation
N 2 aminoethyl maleimide trifluoroacetate salt, by Merck Group (1 mentions)
Aspergillopepsin 1, by Merck Group (1 mentions)
Sodium cyanoborohydride, by Merck Group (1 mentions)
Acetonitrile, by Fujifilm (1 mentions)
Milli q water purification system, by Merck Group (1 mentions)
Bovine serum albumin (bsa), by Merck Group (1 mentions)
Axima performance maldi tof tof mass spectrometer, by Shimadzu (1 mentions)
3 protocols using equine apomyoglobin
1
Protein Purification Reagents and Materials
2
Affinity-Based Protein Purification
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The matrix material 5-amino-1-naphthol (5,1-ANL) was purchased from Tokyo Chemical Industry (Tokyo, Japan). Acetonitrile was purchased from Wako Pure Chemicals (Osaka, Japan). Water used in all experiments was purified using a MilliQ water purification system from Millipore (Billerica, MA, USA). Bovine serum albumin (Mr 66,430.3), equine apo-myoglobin (Mr 16,951.4) and human thioredoxin with His-Tag (GSSHHHHHHSSGLVPRGSH) (Mr 13,769.6) were purchased from Sigma-Aldrich (St. Louis, MO, USA). All reagents were used without further purification.
3
MALDI-TOF Mass Spectrometry of PSII Complex
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All reagents and solvents were purchased from Sigma-Aldrich (Saint Quentin-Fallavier, France) with the highest purity available. Peptides and protein used for calibration were purchased from LaserBio Labs (TOF Mix) and Sigma (equine apomyoglobin), respectively. For intact mass analysis, 1 µL of PSII complex prepared at a concentration of ~100 µg Chl /mL in the medium mentioned above was mixed with 2 µL of a saturated solution of sinapinic acid in 60/0.1 acetonitrile/trifluoroacetic acid. Two microliters of this premix were spotted onto the sample plate and allowed to dry under a gentle air stream. Spectra were acquired in positive reflectron and linear mode on an Axima Performance MALDI-TOF/TOF mass spectrometer (Shimadzu, Manchester, UK) with a pulse extraction fixed at 4000 for 3000-10000 m/z range and at 10000 for 6000-20000 m/z range acquisitions.
All spectra were externally calibrated using a homemade calibrant mixture prepared by mixing 1 µL of 50 µM apomyoglobine in water with 2 µL of TOF Mix solution containing ACTH peptide at a concentration of 6 µM.
All spectra were externally calibrated using a homemade calibrant mixture prepared by mixing 1 µL of 50 µM apomyoglobine in water with 2 µL of TOF Mix solution containing ACTH peptide at a concentration of 6 µM.
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