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Control non targeting sirna

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Control non-targeting siRNA is a laboratory reagent designed to serve as a negative control in RNA interference (RNAi) experiments. It is a synthetic, non-targeting small interfering RNA (siRNA) sequence that does not have complementarity to any known gene targets in the cell.

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Lab products found in correlation

Lipofectamine rnaimax, by Thermo Fisher Scientific (2 mentions) Aurka, by Cell Signaling Technology (1 mentions) Bcl xl, by Thermo Fisher Scientific (1 mentions) Bcl2 associated x (bax), by Cell Signaling Technology (1 mentions) Sirna, by Santa Cruz Biotechnology (1 mentions) αγ tubulin, by Merck Group (1 mentions) Na3vo4, by Merck Group (1 mentions)

2 protocols using control non targeting sirna

1

Transfection of Apoptosis-Related Genes

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Transfections were performed in A375 and Hs294T cells using Lipofectamine RNAi Max (Thermofisher) according to the manufacturer’s protocol. We used siRNA targeting p53 (Santa Cruz Biotechnology), BAX (Cell Signaling), PUMA (Cell Signaling), AURKA (Cell Signaling), p21 (Cell Signaling), Bak (Cell Signaling), BCL-xL (Thermo Fisher), and control non-targeting siRNA (Cell Signaling).
Bharti V., Watkins R., Kumar A., Shattuck-Brandt R.L., Mossing A., Mittra A., Shen C., Tsung A., Davies A., Hanel W., Reneau J.C., Chung C., Sizemore G.M., Richmond A., Weiss V.L, & Vilgelm A.E. (2022). BCL-xL inhibition potentiates cancer therapies by redirecting the outcome of p53 activation from senescence to apoptosis. Cell reports, 41(12), 111826.
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2

Probing FAK, MEK, and ERK Signaling

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Polyclonal αpY397-FAK (Abcam), monoclonal αFAK (EMD Millipore), polyclonal αpS298-MEK (Cell Signaling), monoclonal αMEK (GeneTex), monoclonal αphospho-ERK1/2 (pT202/Y204) and polyclonal αERK1/2 (Cell Signaling), monoclonal αmyc (9E10, Santa Cruz Biotechnology), and αγ-tubulin (Sigma-Aldrich) were used for immunoblotting. Na3VO4 was purchased from Sigma. siRNA and primers for PTP-PEST were purchased from Santa Cruz Biotechnology. Control nontargeting siRNA was purchased from Cell Signaling. Human PRL was purchased from the National Hormone and Peptide Program (Dr. Parlow, National Institute of Diabetes and Digestive and Kidney Diseases).
Hammer A, & Diakonova M. (2016). Prolactin-induced PAK1 tyrosyl phosphorylation promotes FAK dephosphorylation, breast cancer cell motility, invasion and metastasis. BMC Cell Biology, 17, 31.
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