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Dulbecco s modified eagle s medium dmem eagle s minimum essential medium emem

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Dulbecco's modified Eagle's medium (DMEM) and Eagle's Minimum Essential Medium (EMEM) are cell culture media used to support the growth of various cell types in vitro. DMEM and EMEM provide a balanced salt solution, amino acids, vitamins, and other nutrients required for cell survival and proliferation. These media are commonly used in cell biology research and biotechnology applications.

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Lab products found in correlation

Trypan blue, by Merck Group (1 mentions) Hb 8065, by American Type Culture Collection (1 mentions) Htb 26, by American Type Culture Collection (1 mentions) Htb 22, by American Type Culture Collection (1 mentions) Crl 1619, by American Type Culture Collection (1 mentions) Mda mb 231, by American Type Culture Collection (1 mentions) Ethanol, by Merck Group (1 mentions) Formic acid, by Merck Group (1 mentions) Acetonitrile, by Merck Group (1 mentions) Trypsin edta, by Merck Group (1 mentions) Trifluoroacetic acid, by Merck Group (1 mentions) Phosphate buffered saline (pbs), by Merck Group (1 mentions) Crl 6322, by American Type Culture Collection (1 mentions) Kojic acid, by Merck Group (1 mentions) Synthetic melanin, by Merck Group (1 mentions) Mccoy s 5a medium, by LGC (1 mentions) Dulbecco s phosphate buffered saline, by Merck Group (1 mentions) Fetal bovine serum (fbs), by PAN Biotech (1 mentions) Sodium hydroxide (naoh), by Honeywell (1 mentions) Acetic acid, by Honeywell (1 mentions)

Close spelling variants of this product

Dulbecco's Modified Eagle's Minimum Essential Medium Eagle’s minimum essential medium (EMEM), Dulbecco’s minimum essential medium (DMEM), Dulbecco’s modified essential medium (DMEM, Dulbecco’s minimum essential medium Dulbecco’s modified essential medium Dulbecco‐modified essential medium (DMEM) Dulbecco’s modified EMEM medium DMEM medium

2 protocols using dulbecco s modified eagle s medium dmem eagle s minimum essential medium emem

1

Characterization of Cancer Cell Lines

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The cancer cell lines used in the present study, B16-F0 [murine melanoma; CRL-6322™, American Type Culture Collection (ATCC), Manassas, VA, USA], B16 melanoma 4A5 (murine melanoma; 94042254; Sigma-Aldrich Chemie GmbH, Munich, Germany), A375 (human melanoma; CRL-1619™; ATCC), HepG2 (human liver carcinoma; HB8065™; ATCC), MCF7 (human breast carcinoma; HTB22™; ATCC) and MDA-MB-231 (human breast carcinoma; HTB26™; ATCC), were acquired from Sigma-Aldrich Chemie GmbH and ATCC as frozen items.
The specific reagents for cell culture [Dulbecco's modified Eagle's medium (DMEM); Eagle's Minimum Essential Medium (EMEM)], fetal bovine serum (FBS), antibiotic mixture of penicillin/streptomycin, phosphate-buffered saline (PBS), Trypsin/EDTA and trypan blue were acquired from Sigma-Aldrich Chemie GmbH and ATCC.
Ethanol, formic acid, trifluoroacetic acid and acetonitrile were acquired from Sigma-Aldrich Chemie GmbH. α-cyano-4-hydroxycinnamic acid (Bruker HCCA matrix) and protein I calibration standard were acquired from Bruker Daltonics (Bremen, Germany).
Serafim V., Shah A., Puiu M., Andreescu N., Coricovac D., Nosyrev A.E., Spandidos D.A., Tsatsakis A.M., Dehelean C, & Pinzaru I. (2017). Classification of cancer cell lines using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry and statistical analysis. International Journal of Molecular Medicine, 40(4), 1096-1104.
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2

Cannabinoid-Pigment Interaction Assay

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Dulbecco’s phosphate-buffered saline (DPBS), neutral red solution (3.3 g/L in DPBS), Dulbecco’s modified Eagle’s medium (DMEM), Eagle’s minimum essential medium (EMEM), synthetic melanin, glucose, L-3,4-dihydroxyphenylalanine (L-DOPA), murine tyrosinase, kojic acid (≥98.5%) were acquired from Sigma Aldrich/Merck (Darmstadt, Germany). Fetal bovine serum (FBS) was from Pan Biotech (Aidebach, Germany), whereas McCoy’s 5a medium was from LGC Standards (Łomianki, Poland). Ethanol, acetic acid, and sodium hydroxide (NaOH) were purchased from Honeywell (Charlotte, NC, USA). Cannabidiol (CBD, >99.0%), cannabigerol (CBN, >98.0%), cannabinol (CBN, >98.5%), and cannabichromene (CBC, >95.0%) were isolated from hemp flower extracts, as described in [32 (link),33 (link)].
Gaweł-Bęben K., Czech K, & Luca S.V. (2023). Cannabidiol and Minor Phytocannabinoids: A Preliminary Study to Assess Their Anti-Melanoma, Anti-Melanogenic, and Anti-Tyrosinase Properties. Pharmaceuticals, 16(5), 648.
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