Whole prostates were fixed in 4% formaldehyde (Electron Microscopy Sciences, Hatfield, PA) and embedded in paraffin. H&E staining was performed as previously described (72 (link)). Antigen was retrieved by heating slides in 0.01 M sodium citrate buffer (pH 6.0). Primary antibodies for INPP4B (Santa Cruz Biotechnology) or AR (Santa Cruz Biotechnology) were used at 1:800 dilution and sections were counterstained with hematoxylin (EMD Millipore). All images were acquired by AxioCam camera and were processed by AxioVision LE software (Zeiss).
Primary antibodies for inpp4b
Manufactured by Santa Cruz Biotechnology
INPP4B primary antibodies are laboratory reagents used to detect and quantify the expression of the INPP4B protein in biological samples. INPP4B, or inositol polyphosphate-4-phosphatase type II, is an enzyme involved in the regulation of cellular signaling pathways. These primary antibodies can be used in various immunoassay techniques, such as Western blotting, immunohistochemistry, and flow cytometry, to study the expression and localization of INPP4B in different cell types and tissues.
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2 protocols using primary antibodies for inpp4b
1
Immunohistochemical Analysis of Prostate Tissue
2
Immunohistochemical Analysis of Prostate Tissue
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Whole prostates were fixed in 4% formaldehyde (Electron Microscopy Sciences, Hatfield, PA) and embedded in paraffin. H&E staining was performed as previously described (72 (link)). Antigen was retrieved by heating slides in 0.01 M sodium citrate buffer (pH 6.0). Primary antibodies for INPP4B (Santa Cruz Biotechnology) or AR (Santa Cruz Biotechnology) were used at 1:800 dilution and sections were counterstained with hematoxylin (EMD Millipore). All images were acquired by AxioCam camera and were processed by AxioVision LE software (Zeiss).
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