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Pepmix hiv nef ultra

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The PepMix™ HIV NEF Ultra is a laboratory product developed by JPT Peptide Technologies. It is a collection of synthetic peptides derived from the HIV Nef protein. The core function of this product is to provide a standardized and well-characterized set of peptides for research purposes, without interpretation or extrapolation on its intended use.

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Lab products found in correlation

Cd107a, by BD (2 mentions) Cd49d l25, by BD (2 mentions) Cd4 t4d11 ecd, by Beckman Coulter (2 mentions) T bet 4b10 ax647 unlabeled cd28 l293, by BD (2 mentions) Eomesodermin wd1928 pe efluor610, by Thermo Fisher Scientific (2 mentions) Unlabeled functional grade cd3 okt3, by Thermo Fisher Scientific (2 mentions) Perforin b d48 pe, by Cell Sciences (2 mentions) Unlabeled pan anti tgf β cat no t9429, by Merck Group (2 mentions) Rabbit igg cat no i5006, by Merck Group (2 mentions) T bet, by BioLegend (2 mentions) Staphylococcal enterotoxin b, by Merck Group (2 mentions) Click it plus alexa fluor picolyl azide toolkit, by Thermo Fisher Scientific (1 mentions) Ucht1, by Bio-Rad (1 mentions) Lsrii flow cytometer, by BD (1 mentions)

3 protocols using pepmix hiv nef ultra

1

Multiparameter Flow Cytometry Panel

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The following fluorochrome-labeled monoclonal antibodies were used in flow cytometry: CD107a (H4A3: PE-Cy5, PE-Cy7), CD8 (SK1: APC-H7, FITC), CCR7 (3D12: PE-Cy7), Granzyme B (BG11: V450) from BD Biosciences (San Jose, CA); CD4 (T4D11: ECD) from Beckman Coulter (Brea, CA); CD4 (RPA-TA: BV785), CD45RO (UCHL1: BV785), CD27 (O323: BV650), Granzyme A (CB9: Ax647), CD3 (UCHT-1: Ax700), and T-bet (4B10: BV711) from Biolegend (San Diego, CA). CD3 (S4.1: Qdot655) was purchased from Invitrogen (Carlsbad, CA). T-bet (4B10: Ax647); unlabeled CD28 (L293), and CD49d (L25) were from BD Pharmingen (San Diego, CA). Eomesodermin (WD1928: PE-eFluor610) and unlabeled functional grade CD3 (OKT3) were from eBiosciences (San Diego, CA). Perforin (B-D48: PE) was from Cell Sciences (Canton, MA). Unlabeled pan anti-TGF-β (Cat No. T9429) and rabbit IgG (Cat No. I5006) were from Sigma Aldrich. The HIV Gag peptide pool (p55, HXB2 sequence) consisted of 15-mers with an 11 amino acid overlap (BD Biosciences). HIV Nef peptide pool (PepMix™ HIV NEF Ultra, JPT Peptide Technologies, Berlin, Germany) consisted of 150 15-mers with an 11 amino acid overlap. Staphylococcal enterotoxin B (SEB) was from Sigma Aldrich.
Kiniry B.E., Ganesh A., Critchfield J.W., Hunt P.W., Hecht F.M., Somsouk M., Deeks S.G, & Shacklett B.L. (2016). Predominance of Weakly Cytotoxic, T-betLowEomesNeg CD8+ T-cells in Human Gastrointestinal Mucosa: Implications for HIV Infection. Mucosal immunology, 10(4), 1008-1020.
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2

Protein Translation Assay for HIV Peptides

Cited 1 time
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The protein translation assay was adapted from Araki et al.(67 (link)). Briefly, LNMCs were rested overnight and incubated for 30 minutes in methionine-free RPMI medium supplemented with 10% fetal bovine serum, 1% L-glutamine, and 1% penicillin/streptomycin. The cultures were then supplemented with Click-iT HPG (100 μM, Thermo Fisher Scientific). For stimulation conditions, overlapping HIV peptide pools at a final concentration of 2μg/mL/peptide (PepMix HIV (NEF) Ultra or PepMix HIV (GAG) Ultra, JPT Peptide Technologies) or anti-CD3 antibody (UCHT1, BioRad) were used in the presence of brefeldin A and monensin. Cells were harvested after a further 6 hours, stained as described above (see Antibodies), and acquired using an LSRII flow cytometer (BD Biosciences), following the instructions in the Click-iT Plus Alexa Fluor Picolyl Azide Toolkit (Thermo Fisher Scientific).
Nguyen S., Deleage C., Darko S., Ransier A., Truong D., Agarwal D., Japp A.S., Wu V.H., Kuri-Cervantes L., Abdel-Mohsen M., Del Rio Estrada P.M., Ablanedo-Terrazas Y., Gostick E., Hoxie J.A., Zhang N.R., Naji A., Reyes-Terán G., Estes J.D., Price D.A., Douek D.C., Deeks S.G., Buggert M, & Betts M.R. (2019). CD8+ T cells in lymphoid tissues exhibit distinct functional and transcriptional signatures that associate with elite control of HIV. Science translational medicine, 11(523), eaax4077.
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3

Multiparameter Flow Cytometry Panel

Check if the same lab product or an alternative is used in the 5 most similar protocols
The following fluorochrome-labeled monoclonal antibodies were used in flow cytometry: CD107a (H4A3: PE-Cy5, PE-Cy7), CD8 (SK1: APC-H7, FITC), CCR7 (3D12: PE-Cy7), Granzyme B (BG11: V450) from BD Biosciences (San Jose, CA); CD4 (T4D11: ECD) from Beckman Coulter (Brea, CA); CD4 (RPA-TA: BV785), CD45RO (UCHL1: BV785), CD27 (O323: BV650), Granzyme A (CB9: Ax647), CD3 (UCHT-1: Ax700), and T-bet (4B10: BV711) from Biolegend (San Diego, CA). CD3 (S4.1: Qdot655) was purchased from Invitrogen (Carlsbad, CA). T-bet (4B10: Ax647); unlabeled CD28 (L293), and CD49d (L25) were from BD Pharmingen (San Diego, CA). Eomesodermin (WD1928: PE-eFluor610) and unlabeled functional grade CD3 (OKT3) were from eBiosciences (San Diego, CA). Perforin (B-D48: PE) was from Cell Sciences (Canton, MA). Unlabeled pan anti-TGF-β (Cat No. T9429) and rabbit IgG (Cat No. I5006) were from Sigma Aldrich. The HIV Gag peptide pool (p55, HXB2 sequence) consisted of 15-mers with an 11 amino acid overlap (BD Biosciences). HIV Nef peptide pool (PepMix™ HIV NEF Ultra, JPT Peptide Technologies, Berlin, Germany) consisted of 150 15-mers with an 11 amino acid overlap. Staphylococcal enterotoxin B (SEB) was from Sigma Aldrich.
Kiniry B.E., Ganesh A., Critchfield J.W., Hunt P.W., Hecht F.M., Somsouk M., Deeks S.G, & Shacklett B.L. (2016). Predominance of Weakly Cytotoxic, T-betLowEomesNeg CD8+ T-cells in Human Gastrointestinal Mucosa: Implications for HIV Infection. Mucosal immunology, 10(4), 1008-1020.
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