Mouse brains were sectioned sagittally down the midline and fixed in formalin. Tissue blocks were paraffin-embedded and cut into 5µm sections. Following antigen retrieval, tissues were blocked and incubated with α-Ki67 (1:500; Vector, VP-RMO4) or Ccnd1 (1:100; Thermo Scientific, SP4) overnight at 4°C, then incubated with biotinylated goat anti-rabbit IgG (1:300; Vector, BA-1000). Slides were stained with hematoxylin and mounted using VectaMount (Vector). Images were captured with an Olympus DP70 digital camera, and were analyzed using the DP Controller software package (Olympus). Images were processed for publication using Adobe Photoshop Elements 5.0 (Adobe Systems).
Dp controller software package
Manufactured by Olympus
The DP Controller software package is a digital imaging and analysis tool designed for Olympus microscopes. It allows users to capture, process, and analyze images from Olympus microscope systems. The software provides essential image acquisition and management functions.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using dp controller software package
1
Visualizing Cell Proliferation in Mouse Brain
2
Visualizing Cell Proliferation in Mouse Brain
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Mouse brains were sectioned sagittally down the midline and fixed in formalin. Tissue blocks were paraffin-embedded and cut into 5µm sections. Following antigen retrieval, tissues were blocked and incubated with α-Ki67 (1:500; Vector, VP-RMO4) or Ccnd1 (1:100; Thermo Scientific, SP4) overnight at 4°C, then incubated with biotinylated goat anti-rabbit IgG (1:300; Vector, BA-1000). Slides were stained with hematoxylin and mounted using VectaMount (Vector). Images were captured with an Olympus DP70 digital camera, and were analyzed using the DP Controller software package (Olympus). Images were processed for publication using Adobe Photoshop Elements 5.0 (Adobe Systems).
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