Dmem medium with high glucose

Manufactured by Thermo Fisher Scientific

Sourced in United States

DMEM medium with high glucose is a commonly used cell culture medium that provides a balanced salt solution and essential nutrients to support the growth and maintenance of a variety of cell types. It contains a high concentration of glucose as an energy source for cells.

Automatically generated - may contain errors

Lab products found in correlation

5 protocols using dmem medium with high glucose

Evaluating Osteogenesis and Osteoclastogenesis

Check if the same lab product or an alternative is used in the 5 most similar protocols

MC3T3-E1 cells, the murine osteoblast-like cell line, were obtained from the Shanghai Cellular Institute of China Scientific Academy and used for evaluating osteogenesis activity. Cells were cultured in an α-MEM medium (Gibco, USA) containing 10% fetal bovine serum (FBS, Gibco, USA) and 1% penicillin-streptomycin (Gibco, USA).
As the murine preosteoclast cell line, RAW 264.7 cells used for evaluating osteoclastogenesis activity were obtained from Shanghai Zhong Qiao Xin Zhou Biotechnology Co, Ltd. Cells were maintained in DMEM medium with high glucose (Gibco, USA) supplemented with 10% FBS (Gibco, USA) and 1% penicillin-streptomycin (Gibco, USA). The osteoclastogenesis inducing medium containing 50 ng/mL receptor activator of nuclear factor kappa-B ligand (RANKL) and 20 ng/mL macrophage colony stimulating factor (m-CSF) were added.

Yu X., Xu R., Zhang Z., Jiang Q., Liu Y., Yu X, & Deng F. (2021). Different Cell and Tissue Behavior of Micro-/Nano-Tubes and Micro-/Nano-Nets Topographies on Selective Laser Melting Titanium to Enhance Osseointegration. International Journal of Nanomedicine, 16, 3329-3342.

+ Open protocol

+ Expand

Cell Culture Protocols for MCF-7 and CCRF-CEM

Check if the same lab product or an alternative is used in the 5 most similar protocols

MCF-7 adherent cells (human breast adenocarcinoma line) were cultured in DMEM medium with high glucose (Gibco), supplemented with 10 % fetal bovine serum (Gibco), 2 mM l-glutamine, antibiotics (100 U/cm³ penicillin, 100 µg/cm³ streptomycin) and 10 µg/cm³ of human recombinant insulin. CCRF-CEM cell suspension (human T lymphoblast cell line) was cultured in RPMI 1640 medium (Gibco) supplemented with 10 % fetal bovine serum and antibiotics (100 U/cm³ penicillin, 100 µg/cm³ streptomycin). Cells were grown in 25 cm² culture flasks (Sarstedt), in a humidified atmosphere of CO₂/air (5/95 %) at 37 °C.

Łukowska-Chojnacka E., Wińska P., Wielechowska M, & Bretner M. (2016). Synthesis of polybrominated benzimidazole and benzotriazole derivatives containing a tetrazole ring and their cytotoxic activity. Monatshefte Fur Chemie, 147(10), 1789-1796.

+ Open protocol

+ Expand

Cell Culture Protocols for Cancer and Normal Cell Lines

Check if the same lab product or an alternative is used in the 5 most similar protocols

The human embryonic kidney cell line HEK293T, human breast cancer cell lines MCF7, MDA-MB-231, BT549, and SKBR3, and human normal breast epithelial cell line MCF10A were purchased from the Chinese Academy of Science, Shanghai (China). The MCF7, MDA-MB-231, and 293 T cells were cultured in DMEM medium with high glucose (Gibco, Thermo Fisher Scientific, Waltham, MA, USA), while the BT549 and MCF10A cells were cultured in RPMI-1640 medium (Gibco, USA) and mammary epithelial cell medium (Procell, Wuhan, China), respectively. The growth media were added with 10% fetal bovine serum (Gibco, USA) and 100 U/mL penicillin and 100 μg/mL streptomycin (Servicebio, Wuhan, China). A 0.25% trypsin-EDTA solution (Servicebio, China) was also applied for cell passage cultivation. All cell lines were cultivated in a cell incubator at 37 °C and 5% CO₂.

Deng X., Ye D., Hua K., Song H., Luo Q., Munankarmy A., Liu D., Zhou B., Zheng W., Zhou X., Ji C., Wang X., Yu Y, & Fang L. (2021). MIR22HG inhibits breast cancer progression by stabilizing LATS2 tumor suppressor. Cell Death & Disease, 12(9), 810.

+ Open protocol

+ Expand

Cell Culture of Liver and Kidney Lines

Check if the same lab product or an alternative is used in the 5 most similar protocols

Human hepatocellular carcinoma cell lines (SMMC-7721, MHCC97, Bel-7402, Huh7, and Hep3B cells), normal hepatocytes (HL-7702 and THLE-3 cells) and HEK 293T cell lines were obtained from Shanghai Cell Collection, Chinese Academy of Sciences. HL-7702, THLE-3 cells, SMMC-7721, MHCC97, and Bel-7402 cells were cultured in RPMI-1640 medium (Gibco, Grand Island, NY, USA). Huh7, Hep3B, and HEK 293T cells were cultured in DMEM medium with high glucose (Gibco). All culture medium was supplemented with 10% fetal bovine serum (FBS; HyClone, Logan, UT, USA), 100 U/ml penicillin, and 100 μg/ml streptomycin. Cells were cultured in a humidified incubator at 37°C with 5% CO₂.

Jiang C., Liu X., Wang M., Lv G, & Wang G. (2019). High Blood miR-802 Is Associated With Poor Prognosis in HCC Patients by Regulating DNA Damage Response 1 (REDD1)-Mediated Function of T Cells. Oncology Research, 27(9), 1025-1034.

+ Open protocol

+ Expand

HepG2 and Huh-7 Cell Culture with Sorafenib

Check if the same lab product or an alternative is used in the 5 most similar protocols

The human HCC cell lines HepG2 and Huh-7 were obtained from the Cell Bank of the Chinese Academy of Sciences and were grown in DMEM medium with high glucose (Gibco BRL, Grand Island, NY) in the presence of 100 U/mL penicillin, 100 μg/mL streptomycin, and 10% heat-inactivated fetal bovine serum (Invitrogen, Carlsbad, CA, USA). The cells were incubated at 37°C in a humidified atmosphere containing 5% CO₂.
Sorafenib was purchased from Selleck Chemicals (Houston, TX, USA) and dissolved in 100% dimethylsulfoxide (DMSO) (Saint Louis, MO, USA).

Xu G.L., Ni C.F., Liang H.S., Xu Y.H., Wang W.S., Shen J., Li M.M, & Zhu X.L. (2020). Upregulation of PD-L1 expression promotes epithelial-to-mesenchymal transition in sorafenib-resistant hepatocellular carcinoma cells. Gastroenterology Report, 8(5), 390-398.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!