Sunfire c18 reverse phase column

Manufactured by Waters Corporation

The SunFire-C18 reverse-phase column is a high-performance liquid chromatography (HPLC) column designed for the separation and analysis of a wide range of organic compounds. The column features a chemically bonded C18 stationary phase, which provides excellent retention and selectivity for a variety of analytes. The column dimensions and packing material are optimized for efficient and reproducible separations.

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Lab products found in correlation

Waters 2998, by Waters Corporation (3 mentions) Waters alliance e2695 separations module, by Waters Corporation (3 mentions) Zorbax eclipse xdb c18 reverse phase column, by Agilent Technologies (2 mentions) Hypersil gold c18, by Thermo Fisher Scientific (1 mentions) Agilent 1260 lc, by Agilent Technologies (1 mentions)

7 protocols using sunfire c18 reverse phase column

HPLC Analysis of Organic Compounds

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Example 1

Analysis of the Products by C18 Reverse Phase HPLC (Method A)

HPLC analysis was carried out on SunFire™ C18 reverse phase column (4.6×50 mm, 3.5 μm) (Waters Corp., Millford, Mass.) with a mobile phase consisting of water+0.01% TFA (solvent A) and acetonitrile+0.01% trifluoroacetic acid (TFA) (solvent B) at a flow rate of 2.0 mL/minute and column temperature of 50° C. The injection volume was 10 μL and the analyte was detected using UV at 220 and 254 nm. The initial gradient was 5% solvent B, increased to 95% solvent B within 1.4 minutes, then maintained at 95% solvent B for 1.6 minutes. All solvents were HPLC grade.

Analysis of the Product by C18 Reverse Phase HPLC (RPHPLC) (Method B).

RPHPLC analysis was carried out on Zorbax® Eclipse XDB-C18 reverse phase column (4.6×100 mm, 3.5 μm, Agilent PN: 961967-902, Agilent Technologies, Santa Clara, Calif.) with a mobile phase consisting of water+0.1% TFA (solvent A) and acetonitrile+0.1% TFA (solvent B) at a flow rate of 1.5 mL/minute and column temperature of 35° C. The injection volume was 10 μL and the analyte was detected using UV at 220 and 254 nm. The initial gradient was 5% solvent B, increased to 95% solvent B within 6 minutes, and maintained at 95% solvent B for 2 minutes, then returned to 5% solvent B and maintained for 2 minutes.

US10322137B2. Platinum compounds, compositions, and uses thereof (2019-06-18). PLACON THERAPEUTICS, INC. [US]. Inventors: Mark T. Bilodeau [US], Benoit Moreau [US], Adam H. Brockman [US], Kristan Meetze [US], Kerry Whalen [US], Richard Wooster [US], Rossitza G. Alargova [US].

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Quantitative Analysis of Drug-Carrier Systems

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The amount
of encapsulated and released drug, as well as the release of the corresponding
organic linker was determined using a reversed-phase HPLC system Waters
Alliance e2695 Separations Module (Waters, Milford, MA), equipped
with a variable-wavelength photodiode array detector Waters 2998 and
controlled by Empower software. SunFire-C18 reverse-phase column (5
μm, 4.6 × 150 mm², Waters) or Hypersil GOLD
C18 (5 μm, 150 × 4.6 mm², Thermo Fisher) were
employed. For the quantification of all of the chemical species, isocratic
conditions were used. The flow rate was 0.8 mL·min^–1, and the column temperature was fixed at 25 °C. In all cases,
the injection volume was 50 μL.
Different HPLC measurement
conditions were used for the quantification of drugs and ligands:
AAS (85:15 MeOH/buffer solution (0.04 M, pH = 2.5), retention time
(RT) = 5.6 min and absorption maximum λ = 227.9 nm); IBU (85:15
MeOH/buffer solution (0.04 M, pH = 2.5), RT = 4.9 min and λ
= 219.6 nm); H₃BTC (50:50 MeOH/buffer solution (0.04 M,
pH = 2.5), RT = 3.3 min and λ = 225 nm); H₂BDC (5:95
MeOH/buffer solution (0.04 M, pH = 9), RT = 3.6 min and λ =
240 nm); ABTC (5:95 MeOH/buffer solution (0.04 M, pH = 9), RT = 2.9
min and λ = 225 nm) (SI, Section 1; Figures S1–S5).

Rojas S., Colinet I., Cunha D., Hidalgo T., Salles F., Serre C., Guillou N, & Horcajada P. (2018). Toward Understanding Drug Incorporation and Delivery from Biocompatible Metal–Organic Frameworks in View of Cutaneous Administration. ACS Omega, 3(3), 2994-3003.

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HPLC Analysis of Chemical Products

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Example 1

Analysis of the Products by C18 Reverse Phase HPLC (Method A)

The HPLC analysis was carried out on SunFire™ C18 reverse phase column (4.6×50 mm, 3.5 μm) (Waters Corp., Millford, Mass.) with a mobile phase consisting of water +0.01% TFA (solvent A) and acetonitrile +0.01% trifluoroacetic acid (TFA) (solvent B) at a flow rate of 2.0 mL/minute and column temperature of 50° C. The injection volume was 10 μL and the analyte was detected using UV at 220 and 254 nm. The initial gradient was 5% solvent B, increased to 95% solvent B within 1.4 minutes, then maintained at 95% solvent B for 1.6 minutes. All solvents were HPLC grade.

Analysis of the Product by C18 Reverse Phase HPLC (RPHPLC) (Method B).

RPHPLC analysis was carried out on Zorbax® Eclipse XDB-C18 reverse phase column (4.6×100 mm, 3.5 μm, Agilent PN: 961967-902, Agilent Technologies, Santa Clara, Calif.) with a mobile phase consisting of water +0.1% TFA (solvent A) and acetonitrile +0.1% TFA (solvent B) at a flow rate of 1.5 mL/minute and column temperature of 35° C. The injection volume was 10 μL and the analyte was detected using UV at 220 and 254 nm. The initial gradient was 5% solvent B, increased to 95% solvent B within 6 minutes, and maintained at 95% solvent B for 2 minutes, then returned to 5% solvent B and maintained for 2 minutes.

US09937187B2. Platinum compounds, compositions, and uses thereof (2018-04-10). Placon Therapeutics, Inc. [US]. Inventors: Mark T. Bilodeau [US], Benoît Moreau [US], Adam H. Brockman [US], Kristan Meetze [US], Kerry Whalen [US], Richard Wooster [US], Rossitza G. Alargova [US].

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Measuring DSB Loading in MOF (Mg)

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To measure the amount of DSB loading, 10 mg of DSB@MOF (Mg) was poured into 0.1 M NaOH (10 mL), stirred at ambient temperature for 30 min and centrifuged. Then, 1 mL of supernatant was dissolve in methanol (1 mL) and subsequently filtered via (a 0.45-µm membrane), followed by analyzing through HPLC. Thus, the specifications were Sunfire-C18 reverse-phase column (5 μm, 4.6 × 150 mm Waters), the mobile phase of V (acetonitrile): V (K2HPO4, pH = 2) = 1:1, the flow rate of 1 mL/min, the temperature of 25 °C, and the solution optical density (OD) of 260 nm using Ultraviolet–visible spectroscopy (UV–Vis). The concentration curve of standard DSB solution was applied to obtain the amount of DSB loading, based on the equation as follows (Li et al., 2019a (link)):

D L E % = \frac{M_{t}}{M_{s}} \times 100 %

Herein, Mt stands for total mass of DSB loaded, Ms for total MOF (Mg) content and DLE express Encapsulation/Entrapment Efficiency.

Alsaikhan F., Mahmoud M.Z, & Suliman M. (2023). Synthesis and characterization of novel denosumab/magnesium-based metal organic frameworks nanocomposite prepared by ultrasonic route as drug delivery system for the treatment of osteoporosis. Frontiers in Bioengineering and Biotechnology, 11, 1153969.

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Ketoprofen Loading on Sr/PTA-MOF Nanoparticles

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2.5 g of ketoprofen was dissolved in 20 ml of ethyl alcohol at room temperature, and then 150 mg of Sr/PTA-MOF was added in this solution. After stirring for 24 h, the nanoparticles were obtained by filtration and washed with distilled water, and finally dried at 150 °C for 24 h to further treat. The loading samples were labeled as Sr/PTA-MOFs-Ketoprofen.
Loaded ketoprofen amount in MOFs is measured as following: 5 mg of samples after ketoprofen loading were digested separately in 10 mL of NaOH (0.1 mol/L) overnight. Then 2 mL of supernatant was taken and then mixed with 2 mL of methanol. After filtering by 0.45 µm filter membrane, this solution was tested using Agilent 1260 LC high performance liquid chromatography. The mobile phase was V(acetonitrile): V(K₂HPO₄, pH = 2) = 1:1. And sunfire-C18 reverse-phase column (5 μm, 4.6 × 150 mm Waters) was employed. Besides, the flow rate was 1 mL · min⁻¹ and the column temperature was fixed at 25 °C. Ketoprofen in NaOH solutions (0.05, 0.1, 0.15, 0.2, 0.25, 0.3, 0.35, 0.4, 0.45, 0.5 mg · mL⁻¹) were used as standards.

Li Z., Peng Y., Xia X., Cao Z., Deng Y, & Tang B. (2019). Sr/PTA Metal Organic Framework as A Drug Delivery System for Osteoarthritis Treatment. Scientific Reports, 9, 17570.

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HPLC Detection of Trimesic Acid

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The detection of trimesic acid was carried out in a reversed-phase HPLC system, Waters Alliance e2695 Separations Module (Waters, Milford, MA, USA), equipped with a UV-Vis detector Waters 2998. The system was controlled by Agilent software. SunFire-C18 reverse-phase column (5 μm, 4.6 mm × 150 mm, Waters) was employed. For the analysis of BTC, a mobile phase, A, consisting of a buffer solution (0.04 M, pH = 2.5), and a mobile phase, B, with MeCN (50:50) was used. The injection volume was 50 µL, and the detection wavelength was set at 225 nm. The column temperature was fixed at 25 °C [40 (link)]. The retention time of BTC was 3.1 min.
Buffer preparation: NaH₂PO₄ (2.4 g, 0.02 mol) and Na₂HPO₄ (2.84 g, 0.02 mol) were dissolved in 1 L of Milli-Q water. The pH was then adjusted to 2.5 with H₃PO₄ (≥ 85%).

Christodoulou I., Lyu P., Soares C.V., Patriarche G., Serre C., Maurin G, & Gref R. (2023). Nanoscale Iron-Based Metal–Organic Frameworks: Incorporation of Functionalized Drugs and Degradation in Biological Media. International Journal of Molecular Sciences, 24(4), 3362.

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HPLC Analysis of Prednisolone Sodium Phosphate

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The detection of PP was carried out in a reversed-phase HPLC system, Waters Alliance e2695 Separations Module (Waters, Milford, MA, USA), equipped with a UV-Vis detector Waters 2998. The system was controlled by Agilent software. SunFire-C18 reverse-phase column (5 μm, 4.6 mm × 150 mm, Waters) was employed, and the analysis method was adjusted as previously reported [49 ]. More precisely, an isocratic method with a mobile phase containing A (acetate buffer 0.01 M, pH = 7), B: ACN: C: MeOH (75:20:5 v/v/v) at a flow rate of 0.8 mL min⁻¹ was used. The temperature was fixed at 30 °C, and the injection volume was 20 μL. The sample was monitored by UV absorbance at 254 nm. The retention time of prednisolone sodium phosphate was 6.3 min.

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