Legend max mouse il 17a elisa kit

Manufactured by BioLegend

The LEGEND MAX™ Mouse IL-17A ELISA Kit is a quantitative sandwich enzyme-linked immunosorbent assay (ELISA) designed for the measurement of mouse interleukin-17A (IL-17A) levels in cell culture supernatants, serum, and plasma samples.

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Lab products found in correlation

Ionomycin, by Thermo Fisher Scientific (1 mentions) Phorbol myristate acetate (pma), by Merck Group (1 mentions) Legend max human il 17a elisa kit, by BioLegend (1 mentions) Legendplex panels, by BioLegend (1 mentions) Facsaria 3, by BD (1 mentions)

Close spelling variants of this product

Legend Max Mouse IFNγ or IL-17A ELISA kits Mouse IL-17A ELISA MAX IL-17A ELISA kit ELISA MAX kits IL-17A Mouse ELISA kits ELISAs

4 protocols using legend max mouse il 17a elisa kit

Quantifying Serum IL-17 Levels

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Serum cytokine IL-17 concentrations were determined using commercially available ELISA kit (BioLegend’s LEGEND MAX™ Mouse IL-17A ELISA Kit). Assays were performed strictly following the manufacturer’s instructions. All samples were measured in duplicates.

Alenazy M.F., Sharif-Askari F.S., El-Wetidy M.S., Sharif-Askari N.S., Hachim I.Y., Temsah M.H., Saddik B., Al-Kufaidy R., Omair M.A., Alshawakir Y.A., Fathaddin A.A., Hannawi S., Hamid Q., Omair M.A., Al-Muhsen S, & Halwani R. (2022). Intranasal administration of abatacept enhances IL-35+ and IL-10+ producing Bregs in lung tissues of ovalbumin-sensitized asthmatic mice model. PLoS ONE, 17(9), e0271689.

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Measuring Plasma and Splenic IL-17 Levels

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To measure plasma IL-17 concentration, serum was collected from recipient NOD scid mice 8 weeks after adoptive transfer. To measure splenic IL-17 secretion, splenocytes (2 × 10⁶) from male NOD mice were seeded in 12-well plates in 5% BSA SFR medium and stimulated with LPA (10 μM) in the presence or absence of Ki16425 (10 μM). After 24 h, the cells were washed and re-stimulated with 1 μg/ml phorbol 12-myristate 13-acetate (PMA, Sigma) and 2 μg/ml ionomycin (Molecular Probes, Invitrogen, Carlsbad, CA, USA) in 0.2% BSA in SFR medium. After 6 h, the culture supernatant was harvested and frozen at −80°C until assayed. IL-17 concentration in serum and cell culture supernatants was measured by LEGEND MAX™ mouse IL-17a ELISA Kit (BioLegend) in accordance with the manufacturer's protocol.

Park E., Kim D., Lee S.M, & Jun H.S. (2017). Inhibition of lysophosphatidic acid receptor ameliorates Sjögren's syndrome in NOD mice. Oncotarget, 8(16), 27240-27251.

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Cytokine Profiling in Murine T Cells

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Enzyme-linked immunosorbent assays (ELISAs) were performed to determine IFN-γ, IL-4 and IL-17A levels in cell culture media of naïve murine CD4+ T cells using commercial kits from Biolegend (San Diego, CA, USA). Similarly, serum IL-17A levels, obtained at day 6 of IMQ treatment, were measured using the LEGEND MAX™ mouse IL-17A ELISA kit (Biolegend).

Shin S.H., Kim H.Y., Yoon H.S., Park W.J., Adams D.R., Pyne N.J., Pyne S, & Park J.W. (2020). A Novel Selective Sphingosine Kinase 2 Inhibitor, HWG-35D, Ameliorates the Severity of Imiquimod-Induced Psoriasis Model by Blocking Th17 Differentiation of Naïve CD4 T Lymphocytes. International Journal of Molecular Sciences, 21(21), 8371.

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Quantification of IL-17A in Plasma and Serum

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Secreted levels of human or mouse IL-17A in plasma or serum were determined according to the manufacturer’s instructions (LEGEND MAX Human IL-17A ELISA Kit, LEGEND MAX Mouse IL-17A ELISA Kit, BioLegend). For human samples, plasma samples from patients with psoriasis were used as internal reference controls. For multiplex quantification of cytokines, the bead-based LEGENDplex panels (Human Th17 7-plex Panel; Human Th 12-plex Panel, Mouse Th17 7-plex Panel; IL-1β, IL-23 and IL-12p70 from the Inflammation Panel 1; granzyme A and granzyme B from the CD8/NK Panel, predefined and custom-designed mix-and-match system from BioLegend) were used according to the manufacturer’s instructions. Flow cytometry reading was performed on the FACSAria III (BD). Mean fluorescence intensity values were recorded using LEGENDplex analysis software (version 2021.07.01), and cytokine concentrations (pg ml⁻¹) were interpolated from a five-parameter logistic non-linear curve model using a separate standard curve for each cytokine. For prognostic stratification of IL-17A plasma levels, an optimal cut point was determined in each dataset separately using X-tile^{28 (link)}.

Váraljai R., Zimmer L., Al-Matary Y., Kaptein P., Albrecht L.J., Shannan B., Brase J.C., Gusenleitner D., Amaral T., Wyss N., Utikal J., Flatz L., Rambow F., Reinhardt H.C., Dick J., Engel D.R., Horn S., Ugurel S., Sondermann W., Livingstone E., Sucker A., Paschen A., Zhao F., Placke J.M., Klose J.M., Fendler W.P., Thommen D.S., Helfrich I., Schadendorf D, & Roesch A. (2023). Interleukin 17 signaling supports clinical benefit of dual CTLA-4 and PD-1 checkpoint inhibition in melanoma. Nature Cancer, 4(9), 1292-1308.

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