Tlrl pgcn

Manufactured by InvivoGen

Tlrl-pgcn is a synthetic agonist of the TLR1/TLR2 heterodimer. It is a purified preparation of the acylated lipopeptide Pam3CSK4, which mimics the structure of bacterial lipoproteins. Tlrl-pgcn can be used to stimulate the TLR1/TLR2 signaling pathway in cell-based assays.

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Lab products found in correlation

Poly dg dc, by InvivoGen (1 mentions) Tlrl isdn, by InvivoGen (1 mentions) Poly dg dc, by Thermo Fisher Scientific (1 mentions) Lipofectamine 3000, by Thermo Fisher Scientific (1 mentions) Nigericin, by Merck Group (1 mentions) Sorafenib, by Merck Group (1 mentions) Recombinant ifn γ, by Thermo Fisher Scientific (1 mentions) Z devd fmk, by Santa Cruz Biotechnology (1 mentions) Z vad fmk, by Santa Cruz Biotechnology (1 mentions) Mcc950, by InvivoGen (1 mentions) Tlrl picw, by Merck Group (1 mentions) Ht dna, by Merck Group (1 mentions) Rnase a t1 cocktail, by Thermo Fisher Scientific (1 mentions) S1 nuclease, by Thermo Fisher Scientific (1 mentions) Dnase 1, by Bio-Rad (1 mentions) Nu7441, by Bio-Techne (1 mentions) Lipofectamine 2000, by Thermo Fisher Scientific (1 mentions) N acetyl cysteine (nac), by Merck Group (1 mentions) Pam3csk4, by InvivoGen (1 mentions) Z4250, by Merck Group (1 mentions)

3 protocols using tlrl pgcn

Type-I IFN Activation in Macrophages

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Interferon stimulating DNA (ISD) and Poly(dG:dC) were obtained from InvivoGen (tlrl-pgcn, tlrl-isdn). To activate type-I IFN response in macrophages, ISD or Poly(dG:dC) were mixed with Lipofectamine 3000 (Invitrogen, L3000150) at a ratio of 1:1 (v/w), and then added to cells at a final concentration of 1 μg/ml.

Mirji G., Worth A., Bhat S.A., Sayed M.E., Kannan T., Goldman A.R., Tang H.Y., Liu Q., Auslander N., Dang C.V., Abdel-Mohsen M., Kossenkov A., Stanger B.Z, & Shinde R.S. (2022). The microbiome-derived metabolite TMAO drives immune activation and boosts responses to immune checkpoint blockade in pancreatic cancer. Science immunology, 7(75), eabn0704.

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Immune Activation Protocol Reagents

Cited 1 time

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The following chemicals and drugs were utilized in this study: Talabostat/VbP (MCE #HY-13233A), Lipofectamine 2000 (Invitrogen #11668019), double-stranded alternating copolymer poly(dA:dT) (pdAdT) (Sigma #P0883), poly(I:C) (pIC) (Invivogen #tlrl-picw), HT-DNA (Sigma #D6898), double-stranded homopolymer poly(dA):poly(dT) (Sigma #P9764), poly(dG:dC) (Invivogen #tlrl-pgcn), PAM3CSK4 (Invivogen #tlrl-pms), nigericin (Sigma #N7143), diABZI (MCE #HY-112921B), ANS (MCE #HY-18982), H₂O₂ (Sigma #H1009), MG-132 (MCE #HY-13259), Bortezomib (MCE #HY-10227), MCC950 (Invivogen #inh-mcc), z-VAD-FMK (Santa Cruz #sc-3067), z-DEVD-FMK (Santa Cruz #sc-311558), H-151 (MCE #HY-112693), NAC (Sigma #A9165), KU-44933 (Santa Cruz #sc-202963), NU-7441 (Tocris #3712), Sorafenib (Sigma #SML2633), PLX-4720 (MCE #HY-51424), Doramapimod (MCE #HY-10320), SB-202190 (MCE #HY-10295), and RNA Polymerase III inhibitor (Sigma #557403). gDNA was isolated from the genomic DNA of HEK293T cells. ISD was synthesized from custom oligos as previously described (55 (link)). Recombinant IFNγ was purchased from Peprotech (#300-02). DNase I (Bio-Rad #7326828), S1 Nuclease (Thermofisher #EN0321), and RNase A/T1 Cocktail (Thermofisher #AM2286) were purchased from the indicated vendors. VACV Copenhagen strain WT and ΔF1L were a kind gift of John Bell (56 (link)) and titered by plaque assay.

Zhou J.Y., Sarkar M.K., Okamura K., Harris J.E., Gudjonsson J.E, & Fitzgerald K.A. (2023). Activation of the NLRP1 inflammasome in human keratinocytes by the dsDNA mimetic poly(dA:dT). Proceedings of the National Academy of Sciences of the United States of America, 120(5), e2213777120.

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Assessing Innate Immune Responses in Whole Blood

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Human whole blood was incubated in round-bottom 96-well plates with test compounds or controls for 6 hr and 45 min at a drug to blood volume ratio of 1:40. After incubation, the plasma was stored at −80°C until required.
ELISA was performed on the samples to measure C3a and C5a (Quidel nos. AO32 and AO25) and interleukin-6 (IL-6), IL-8, tumor necrosis factor alpha (TNF-α), and monocyte chemoattractant protein-1 (MCP1) concentrations (Aushon human arrays nos. 101-261-1-AB and 51-100-1-AB) according to the manufacturer’s instructions. Compounds were investigated at 50 μM in triplicates. Assay controls included PBS, zymosan (“alternative pathway,” Sigma no. Z4250), heat aggregated immunoglobulin G (IgG) (“classical pathway,” Tecomedical no. A114), stop solution (“inhibitor,” Tecomedical no. A9576), R848 (InvivoGen no. tlrl-r848), CpG (InvivoGen no. tlrl-2006-1), and poly(dG:dC) (InvivoGen no. tlrl-pgcn).
The stimulation index was calculated relative to PBS-induced values and represented as mean + SD of blood samples originating from 3 donors.

Javanbakht H., Mueller H., Walther J., Zhou X., Lopez A., Pattupara T., Blaising J., Pedersen L., Albæk N., Jackerott M., Shi T., Ploix C., Driessen W., Persson R., Ravn J., Young J.A, & Ottosen S. (2018). Liver-Targeted Anti-HBV Single-Stranded Oligonucleotides with Locked Nucleic Acid Potently Reduce HBV Gene Expression In Vivo. Molecular Therapy. Nucleic Acids, 11, 441-454.

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