Anti nedd9

Manufactured by Abcam

Sourced in United Kingdom

Anti-NEDD9 is a laboratory tool used to detect and quantify the NEDD9 protein. NEDD9 is involved in various cellular processes and its expression levels can be studied using this product. The product provides a reagent to measure NEDD9 levels, but does not include interpretation or recommendations for its use.

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NEDD9 (6 citations)

3 protocols using anti nedd9

EMT Regulation and Src/STAT3 Signaling

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The study protocol was performed according to the principles of the Declaration of Helsinki and the ethics committee of the Affiliated Hospital of Jiangnan University, Wuxi, Jiangsu Province, China. Informed consent was obtained from all patients.
The following primary antibodies were purchased from Abcam (Cambridge, England): anti-E-cad, anti-Vim, anti-Snail, anti-N-cad, anti-Twist, anti-PCNA, anti-Bax, anti-Bcl-2, anti-ZEB1, anti-NEDD9, anti-Myb, and anti-GAPDH. The following primary antibodies were purchased from Cell Signaling Technology (Beverly, MA, USA): anti-t-SRC, anti-p-SRC, anti-t-STAT3, and anti-p-STAT3. PP1, an SRC inhibitor, was purchased from Selleck Chemicals (Houston, TX, USA).

Xue Y., Wu T., Sheng Y., Zhong Y., Hu B, & Bao C. (2021). MicroRNA-1252-5p, regulated by Myb, inhibits invasion and epithelial-mesenchymal transition of pancreatic cancer cells by targeting NEDD9. Aging (Albany NY), 13(14), 18924-18945.

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Western Blot Analysis of NEDD9 Protein

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Total cellular protein was extracted from cell lines transfected for 72 h using RIPA lysis buffer (DBI Bioscience, Shanghai, China) following the manufacturer’s instructions. After protein quantitation using BCA Protein Quantitative Kit (DBI Bioscience), equal amounts of proteins were separated by SDS-PAGE and blotted onto to PVDF membranes (0.45 µm; Millipore, Billerica, MA, USA). The membranes were blocked in 5% skim milk and then incubated overnight at 4°C with anti-NEDD9 (1:1,000; Abcam) or anti-tubulin (Santa Cruz Biotechnologies, Santa Cruz, CA, USA), followed by incubation with horseradish peroxidase-conjugated IgG. An ECL kit (Millipore) was used for detection.

Wang J., Yang W.J., Sun C., Luan Y., Cheng G.H., Li K.L, & Kong F. (2015). siRNA Suppression of NEDD9 Inhibits Proliferation and Enhances Apoptosis in Renal Cell Carcinoma. Oncology Research, 22(4), 219-224.

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Epithelial-Mesenchymal Transition Pathway

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The study protocol was performed according to the principles of the Declaration of Helsinki and the ethics committee of the A liated Hospital of Jiangnan University, Wuxi, Jiangsu Province, China. Informed consent was obtained from all patients.
The following primary antibodies were purchased from Abcam (Cambridge, England): anti-E-cad, anti-Vim, anti-Snail, anti-N-cad, anti-Twist, anti-PCNA, anti-Bax, anti-Bcl-2, anti-ZEB1, anti-NEDD9 and anti-GAPDH. The following primary antibodies were purchased from Cell Signaling Technology (Beverly, MA, USA): anti-t-SRC, anti-p-SRC, anti-t-STAT3, anti-p-STAT3. PP1, an inhibitor of SRC, was purchased from Selleck Chemicals (Houston, TX, USA).

Xue Y., Wu T., Sheng Y., Yao Z., Hu B., & Bao C. (2020). MicroRNA-1252-5p, regulated by Myb, inhibits invasion and epithelial-mesenchymal transition of pancreatic cancer cells by targeting NEDD9 .

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