N succinyl ala ala p nitroanilide

All the chemicals and reagent used in this work were of laboratory and analytical grade. Diphenyl-1-picrylhydrazyl (DPPH), 2, 2′-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS), 3,5-dinitrosalicylic acid (DNSA), α-amylase, acarbose, 4-nitrophenyl butyrate (p-NPB), lipase, orlistat, l-3,4-dihydroxyphenylalanine (L-DOPA), mushroom tyrosinase, kojic acid, N-succinyl-Ala-Ala-p-nitroanilide (AAAPVN), porcine pancreatic elastase, quercetin, acetylcholinesterase (AChE), butyrylcholinesterase (BChE), acetylthiocholine iodide, butyrylcholine iodide, 5,5′-dithiobis-(2-nitrobenzoic acid) (DNTB), and galantamine were purchased from Sigma-Aldrich, USA.

Different chemicals reagents used in this study were purchased from different chemical suppliers. Quercetin, 2, 2-Diphenyl-1-picrylhydrazyl (DPPH) and 2, 2′-Azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS), α-amylase from procine pancreas (Type VI-B), Tyrosinase from mushroom, Lipase from procine pancreas (Type II), 4-nitrophenyl butyrate (p-NPB), Orlistat, Acarbose, α-glucosidase from Saccharomyces cerevisiae (Type-I), p-Nitrophenyl-α-D-glucopyranoside (p-NPG), Sodium carbonate, Kojic acid, l-3,4-dihydroxyphenylalanine (L-DOPA), N-Succinyl-Ala-Ala-p-nitroanilide (AAAPVN), Porcine pancreatic elastase (PPE), Galantamine, Acetylcholinesterase (AChE), Butyrylcholinesterase (BChE), Acetylcholine iodide, Butyrylcholine iodide, 5,5′-dithiobis-(2-nitrobenzoic acid) (DTNB), Acetonitrile (LC-MS grade), Methanol (LC-MS grade), Formic acid (analytical grade), and Acarbose from Sigma-aldrich (USA). The 3,5-Dinitrosalicylic acid (DNSA) from HIMEDIA (India) and Sodium-potassium tartrate from Merc (Germany). Methanol, Sodium Hydroxide, Dimethyl sulfoxide (DMSO), and Starch from Fisher Scientific (India). Aluminum Chloride, Hexane and Gallic Acid from LOBA Chemie (India).