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Elite ingenius

Manufactured by ELITechGroup
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Sourced in France, Italy, Germany

The ELITe InGenius is a compact and versatile laboratory instrument designed for nucleic acid amplification and detection. It performs real-time PCR analysis to identify and quantify specific DNA or RNA sequences. The system is capable of processing multiple samples simultaneously and provides automated data analysis.

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Lab products found in correlation

Sars cov 2 elite mgb kit, by ELITechGroup (2 mentions) Genexpert xpress sars cov 2 pcr assay, by Cepheid (2 mentions) Starmag 96 4 universal cartridge, by Seegene (1 mentions) Maxwell rsc simply rna blood kit, by Promega (1 mentions) Taqpath covid 19 ce ivd rt pcr kit, by Thermo Fisher Scientific (1 mentions) Genefinder covid 19 plus realamp kit, by ELITechGroup (1 mentions) Magna pure compact, by Roche (1 mentions) Sti plus elite mgb kit, by ELITechGroup (1 mentions) Starlet, by Seegene (1 mentions) Allplex sars cov 2 assay, by Seegene (1 mentions) Lightcycler multiplex rna virus master, by Roche (1 mentions) Cobas sars cov 2 test, by Roche (1 mentions) Xpert xpress sars cov 2, by Cepheid (1 mentions) Lightcycler 480 instrument 2, by Roche (1 mentions) Cobas 6800, by Roche (1 mentions) Genexpert, by Cepheid (1 mentions) Quantgene 9600, by Bioer (1 mentions) Magcore hf16, by RBC Bioscience (1 mentions)

14 protocols using elite ingenius

1

Nasopharyngeal RNA Extraction Protocol

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RNA was extracted from nasopharyngeal swabs using the ELITe InGenius® system (ELITechGroup, Puteaux, France) following the manufacturer’s instructions.
Damin F., Galbiati S., Clementi N., Ferrarese R., Mancini N., Sola L, & Chiari M. (2023). Dual-Domain Reporter Approach for Multiplex Identification of Major SARS-CoV-2 Variants of Concern in a Microarray-Based Assay. Biosensors, 13(2), 269.
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2

Sputum Nucleic Acid Extraction

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Nucleic acids were extracted using ELITe InGenius (ELITechGroup, INT030-K) and STARMag 96×4 Universal Cartridge (Seegene, Catalog number). 200 μL of inactivated sputum was loaded onto the device. The nucleic acids were extracted according to manufacturer’s instructions (Elitech, ELITe InGeniusTM Device Manual) using the “generic” protocol and eluted with 100 μL of elution buffer provided by the supplier.
Prajwal P., Neary T., Rohrbach K., Bittel P., Göller P.C., Buch T., Dümcke S, & Keller P.M. (2023). Optimizing mycobacteria molecular diagnostics: No decontamination! Human DNA depletion? Greener storage at 4 °C!. Frontiers in Microbiology, 14, 1104752.
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3

SARS-CoV-2 Diagnostic Testing in Clinical Samples

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SARS-CoV-2 diagnostic testing of clinical nasopharyngeal swabs or other samples by RT-qPCR was conducted from March 14 to 23, 2020, at an outside facility using the Charité protocol. From March 23, 2020, testing was performed internally at Gustave Roussy. The cycle thresholds were collected only for assays performed at Gustave Roussy. Nasopharyngeal swab samples were collected using flocked swabs (Sigma Virocult) and placed in viral transport media. SARS-CoV-2 RNA was detected using one of two available techniques at Gustave Roussy: the GeneFinder COVID-19 Plus RealAmp kit (ELITech Group) targeting three regions (RdRp gene, NC, and envelope genes) on the ELITe InGenius (ELITech Group) or the multiplex real-time RT-PCR diagnostic kit (the Applied Biosystems TaqPath COVID-19 CE-IVD RT-PCR Kit) targeting three regions (ORF1ab, NC, and spike genes) with the following modifications. Nucleic acids were extracted from specimens using automated Maxwell instruments following the manufacturer's instructions (Maxwell RSC simplyRNA Blood Kit; AS1380; Promega). Real-time RT-PCR was performed on the QuantiStudio 5 Dx Real-Time PCR System (Thermo Fisher Scientific) in a final reaction volume of 20 μL, including 5 μL of extracted nucleic acids, according to the manufacturer's instruction.
Fahrner J.E., Lahmar I., Goubet A.G., Haddad Y., Carrier A., Mazzenga M., Drubay D., Alves Costa Silva C., de Sousa E., Thelemaque C., Melenotte C., Dubuisson A., Geraud A., Ferrere G., Birebent R., Bigenwald C., Picard M., Cerbone L., Lérias J.R., Laparra A., Bernard-Tessier A., Kloeckner B., Gazzano M., Danlos F.X., Terrisse S., Pizzato E., Flament C., Ly P., Tartour E., Benhamouda N., Meziani L., Ahmed-Belkacem A., Miyara M., Gorochov G., Barlesi F., Trubert A., Ungar B., Estrada Y., Pradon C., Gallois E., Pommeret F., Colomba E., Lavaud P., Deloger M., Droin N., Deutsch E., Gachot B., Spano J.P., Merad M., Scotté F., Marabelle A., Griscelli F., Blay J.Y., Soria J.C., Merad M., André F., Villemonteix J., Chevalier M.F., Caillat-Zucman S., Fenollar F., Guttman-Yassky E., Launay O., Kroemer G., La Scola B., Maeurer M., Derosa L, & Zitvogel L. (2022). The Polarity and Specificity of Antiviral T Lymphocyte Responses Determine Susceptibility to SARS-CoV-2 Infection in Patients with Cancer and Healthy Individuals. Cancer Discovery, 12(4), 958-983.
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4

RNA Extraction and rRT-PCR Analysis for SARS-CoV-2

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The RNA samples were extracted from nasopharyngeal swab or combined nasopharyngeal/oral swab collected from the patients at King Fahad Specialist Hospital (KFSH), Dammam, Saudi Arabia, between 01 and 30 November 2020. The swabs were collected in Virus Liquid Transport Medium-VTM per manufacture instruction for collecting and handling (Copan, U.S.A). Specimens were transported to the lab in cool box and kept refrigerated for not more than 8 h till time of nucleic acid extraction.
Prior to RNA extraction, collection tubes were vortexed; 200 or 400 µL of VTM were transferred to 2 mL tubes for RNA extraction using Magna Pure Compact (Roche, Germany) or ELITe InGenius (ELITechGroup, France) systems with elution set to 100 µL. Finally, 5 µL of extracted RNA was used as template for the rRT-PCR.
Tombuloglu H., Sabit H., Al-Khallaf H., Kabanja J.H., Alsaeed M., Al-Saleh N, & Al-Suhaimi E. (2022). Multiplex real-time RT-PCR method for the diagnosis of SARS-CoV-2 by targeting viral N, RdRP and human RP genes. Scientific Reports, 12, 2853.
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5

Rapid Dipstick Assay for Leukocyte Esterase in Urine

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The rapid dipstick assay for leukocyte esterase (Biognost, Zagreb, Croatia) was employed to identify the presence of esterase enzyme produced by PMNLs in urine samples, which was used to screen for signs of active inflammation. Utilizing a reference colour chart, the dipstick categorized results into negative, 1+ (approximately 70 PMNL/μL), 2+ (approximately 125 PMNL/μL) and 3+ (approximately 500 PMNL/μL). Additionally, the urine sediment was microscopically examined to assess the number of PMNLs per hpf (original magnification: ×400). For analysis purposes, we categorized the results into less than 10 PMNL/μL, 10–25 PMNL/μL and more than 25 PMNL/μL.
M. genitalium was identified as part of the routine diagnostics for STI infections using the automated platform ELITe InGenius® (ELITechGroup S.p.a., Torino, Italy). The FTD Urethritis plus kit (Fast Track Diagnostics, Luxembourg) was employed during 2018 and 2019, while the STI PLUS ELITe MGB®Kit (ELITechGroup S.p.a., Torino, Italy) was utilized from 2019 until the end of 2023, following the manufacturers’ instructions.
Ljubin-Sternak S., Meštrović T., Marijan T., Anušić M., Šuto S, & Vraneš J. (2024). Detection of Macrolide and/or Fluoroquinolone Resistance Genes in Mycoplasma genitalium Strains Isolated from Men in the Northwest Region of Croatia in 2018–2023. Genes, 15(4), 470.
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6

SARS-CoV-2 Detection: Automated Extraction and PCR

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For the detection of SARS-CoV-2, two automated equipments were used to extract RNA (EliTe InGenius® (ELITech Group, Dieren, The Netherlands) for blood samples, Seegene STARlet (Seegene Inc. Seoul, South Korea) for nasopharyngeal swabs). For RNA isolation, manufacturer-validated reagents (SP200 extraction reagent, STARMAg isolation kit, Seegene Inc, Seoul, South Korea) were used. Allplex™ SARS-CoV-2 Assay (Seegene Inc., Seoul, South Korea) was chosen for multiplex real-time PCR, due to its capability to detect 4 different SARS-CoV-2 genes simultaneously (E-gene, N-gene, RdRP gene, S-gene). PCR tests were considered negative at 40 cycle threshold (Ct) or more.
Gopcsa L., Réti M., Andrikovics H., Bobek I., Bekő G., Bogyó J., Ceglédi A., Dobos K., Giba-Kiss L., Jankovics I., Kis O., Lakatos B., Mathiász D., Meggyesi N., Miskolczi G., Németh N., Paksi M., Riczu A., Sinkó J., Szabó B., Szilvási A., Szlávik J., Tasnády S., Reményi P, & Vályi-Nagy I. (2023). Effective virus-specific T-cell therapy for high-risk SARS-CoV-2 infections in hematopoietic stem cell transplant recipients: initial case studies and literature review. GeroScience, 46(1), 1083-1106.
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7

Multicenter COVID-19 Hospitalization Cohort

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Between August 2020 and January 2021, 517 patients were enrolled in this project when admitted to Hospital de Santa Maria in Lisbon and Hospital de São João in Oporto. Eligibility criteria included patients aged 18 years and above, patients admitted with COVID-19 disease requiring hospitalization, with a positive test for SARS-CoV-2 by nasopharyngeal swabs using quantitative RT-PCR performed in national reference laboratories and in accordance with recommendations from the National Directorate of Health.
The RT-PCR results for the SARS-CoV-2 were obtained through one of the following tests: LightCycler® Multiplex RNA Virus Master (Roche Life Science, Penzberg, Germany) at a LightCycler® 480 Instrument II (Roche Life Science, Penzberg, Germany), Cobas® SARS-CoV-2 Test (Roche® Diagnostics) at a Cobas® 6800 (Roche® Diagnostics), AllplexTM SARS-CoV-2 Master Assay (Seegene Inc.) at a CFX96® Real-Time (Bio-Rad®), GeneFinderTM COVID-19 PLUS RealAmp Kit (OSANG Healthcare Co, Ltd.) at ELITe InGenius® (ELITechGroup®) or QuantGene 9600 (Hangzhou Bioer Technology Co. Ltd.), Xpert®Xpress SARS-CoV-2 (Cepheid®) at a GeneXpert® (Cepheid®).
All eligible patients were followed till the closure of case i.e. either discharge or mortality.
Freitas A.T., Calhau C., Antunes G., Araújo B., Bandeira M., Barreira S., Bazenga F., Braz S., Caldeira D., Santos S.C., Faria A., Faria D., Fraga M., Nogueira-Garcia B., Gonçalves L., Kovalchuk P., Lacerda L., Lopes H., Luís D., Medeiros F., Melo A.M., Melo-Cristino J., Miranda A., Pereira C., Pinto A.T., Pinto J., Proença H., Ramos A., Rato J.P., Rocha F., Rocha J.C., Moreira-Rosário A., Vazão H., Volovetska Y., Guimarães J.T, & Pinto F.J. (2021). Vitamin D-related polymorphisms and vitamin D levels as risk biomarkers of COVID-19 disease severity. Scientific Reports, 11, 20837.
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8

SARS-CoV-2 Screening and Antibody Detection

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Nasopharyngeal swabs were routinely used to screen the workforce and test symptomatic workers and close contacts of a confirmed COVID case. The SARS-CoV-2 ELITe MGB Kit (Elitechgroup, Italy) was used to detect SARS-CoV-2 RNA extracted from nasopharyngeal swabs. Extraction, detection, and quantification by RT-PCR were performed using a commercial automatized platform (Elite InGenius®, Elitechgroup, Italy).
The electrochemiluminescence immunoassay (ECLIA) Elecsys® anti-SARS-CoV-2 was used to detect immunoglobulins (IgG/A/M) for anti-SARS-CoV-2-S (Roche Diagnostics International Ltd, Rotkreuz, Switzerland). The assay uses a recombinant protein representing the RBD of the S antigen in a double-antigen sandwich assay format from SARS-CoV-2 wild-type (WT) (Wuhan-Hu-1) strain. Serum samples of 20 μL are collected using EDTA tubes. The results are expressed as U/mL, the cut-off was 0.8 U/mL, and the upper limit of detection was 250 U/mL. Since the antibody titers elicited in immunized individuals were very high, we tested all serum samples at a dilution of 1:20, in accordance with Roche, so the upper limit of detection was raised to 5000 U/mL and the dynamic range could be extended.
Arsuffi S., Sansone E., Focà E., Storti S., Diaferia T., Bonfanti C., Terlenghi L., Caruso A., Sala E., Castelli F., De Palma G, & Quiros-Roldan E. (2023). Timing and implications for immune response to vaccine in SARS-CoV-2 breakthrough infections. iScience, 26(5), 106716.
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9

Automated SARS-CoV-2 Detection Workflow

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Oro-nasopharyngeal swabs in UTM were fully automated processed for extraction, amplification and result analysis with ELITe InGenius (ELITechGroup, Torino, Italy) according to manufacturer’s directions. Reverse transcription of SARS-CoV-2 RNA and Real-Time PCR assay were performed in one-step process. From March to June 2020 analysed targets of SARS-CoV-2 were RdRp, E and N genes (GeneFinder COVID-19 Plus RealAmp Kit, OSANG Healthcare); from July 2020 analysed targets were RdRp and ORF-8 genes (SARS-CoV-2 ELITe MGB Kit).
Greco M., Suppressa S., Lazzari R.A., Sicuro F., Catanese C, & Lobreglio G. (2021). sFlt-1 and CA 15.3 are indicators of endothelial damage and pulmonary fibrosis in SARS-CoV-2 infection. Scientific Reports, 11, 19979.
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10

Comparative Analysis of SARS-CoV-2 Detection

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Samples from NHP and HCW were tested on two different RT-qPCR methods by either a validated in-house RT-qPCR assay or on the ELITe InGenius® (Elitech, France) platform [9 ]. Samples from ERP were tested with the GeneXpert Xpress SARS-CoV-2 PCR assay (Cepheid Inc., Sunnyvale, USA) according to the instructions of the manufacturer.
Koeleman J.G., Brand H., de Man S.J, & Ong D.S. (2021). Clinical evaluation of rapid point-of-care antigen tests for diagnosis of SARS-CoV-2 infection. European Journal of Clinical Microbiology & Infectious Diseases, 40(9), 1975-1981.
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