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Peroxidase labeled secondary rabbit and mouse antibodies

Manufactured by Cell Signaling Technology
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Sourced in United States

Peroxidase-labeled secondary rabbit and mouse antibodies are used to detect and visualize target proteins in various immunoassays, such as Western blotting and immunohistochemistry. They serve as a detection reagent, binding to the primary antibody that recognizes the protein of interest and producing a colorimetric or chemiluminescent signal upon the addition of a suitable substrate.

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Lab products found in correlation

Bicinchoninic acid (bca), by Thermo Fisher Scientific (2 mentions) Phospho p53, by Cell Signaling Technology (1 mentions) Dnase free rnase, by Merck Group (1 mentions) Cyclin d1, by Cell Signaling Technology (1 mentions) P egfr, by Cell Signaling Technology (1 mentions) Phospho erk, by Cell Signaling Technology (1 mentions) Strophanthidin, by Merck Group (1 mentions) Antibodies for p53, by Cell Signaling Technology (1 mentions) Antibody for bcl2, by Santa Cruz Biotechnology (1 mentions) Total erk, by Cell Signaling Technology (1 mentions) Total akt, by Cell Signaling Technology (1 mentions) β catenin, by Cell Signaling Technology (1 mentions) Supersignal west pico chemiluminescent substrate, by Thermo Fisher Scientific (1 mentions) Vimentin, by Cell Signaling Technology (1 mentions) Phospho akt ser473, by Cell Signaling Technology (1 mentions) Propidium iodide, by Merck Group (1 mentions) Thiazolyl blue tetrazolium bromide, by Merck Group (1 mentions) Rapamycin, by Merck Group (1 mentions) Dihydroethidium dhe, by Thermo Fisher Scientific (1 mentions) Aminoguanidine, by Merck Group (1 mentions)

Close spelling variants of this product

Rabbit antibodies (6 citations) Horseradish peroxidase-labeled anti-mouse and anti-rabbit secondary antibodies (2 citations)

2 protocols using peroxidase labeled secondary rabbit and mouse antibodies

1

Molecular Mechanisms of Cytotoxicity

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Convallatoxin was obtained from Pfaltz & bauer (Waterbury, CT, USA). Peruvoside was purchased from Research Plus, Inc. (Barnegat, NJ, USA). Strophanthidin, Thiazolyl blue tetrazolium bromide (MTT), Cisplatin, Sodium dichromate (Na2Cr2O7.2H2O) [Cr(VI)], Triton X-100, DNAse free RNAse and Propidium Iodide were obtained from Sigma Aldrich (St Louis, MO, USA). Antibodies for p53, phospho-p53, CDK4, Cyclin D1, PARP, XIAP, p-62, phospho-Akt (Ser 473), total-Akt, phospho-ERK, total-ERK, pEGFR, EGFR, β-Catenin, Vimentin, Slug and peroxidase-labeled secondary rabbit and mouse antibodies were obtained from Cell Signaling Technology (Denvers, MA, USA). Antibody for Bcl2 was procured from Santa Cruz Biotechnology (Dallas, TX, USA). Bicinchoninic acid and Supersignal West Pico chemiluminescent substrate was purchased from Thermo Fisher Scientific (Waltham, MA, USA).
Kaushik V., Azad N., Yakisich J.S, & Iyer A.K. (2017). Antitumor effects of naturally occurring cardiac glycosides convallatoxin and peruvoside on human ER+ and triple-negative breast cancers. Cell Death Discovery, 3, 17009-.
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2

Pharmacological Modulation of Cellular Pathways

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Amino guanidine (AG), Thiazolyl blue tetrazolium bromide (MTT), Cisplatin, Rapamycin, Bafilomycin A1, Sodium dichromate (Na2Cr2O7.2H2O) (Cr[VI]), Pan-caspase inhibitor zVAD.FMK, and Akt-inhibitor LY294002 were obtained from Sigma-Aldrich (St Louis, MO). Mn(III) tetrakis (4-benzoic acid) porphyrin (MnTBAP) and Bcl-2 inhibitor VI (ABT-737) were purchased from Calbiochem (La Jolla, CA). The oxidative probes, 4,5-Diaminofluorescein diacetate (DAF-2DA), Dichlorofluorescein (DCF) diacetate, Dihydroethidium (DHE), and the apoptosis dye Hoechst 33342 were from Molecular Probes (Eugene, OR). Antibodies for Beclin-1, p38 MAPK, GAPDH, p-Akt Ser 473, total-Akt, NF-κB, and peroxidase-labeled secondary rabbit and mouse antibodies were from Cell Signaling Technology. Antibody for superoxide dismutase (SOD) was procured from Sigma Aldrich. Antibodies for Bcl-2 and LC3 were from Santa Cruz Biotechnology and Novus Biologicals, respectively. Cyto-ID® autophagy detection kit was purchased from Enzo Life Sciences. Bicinchoninic acid (BCA) was obtained from Thermo Scientific and chemiluminescent substrate from Pierce (Supersignal® West Pico, Pierce).
Kulkarni Y.M., Kaushik V., Azad N., Wright C., Rojanasakul Y., O’Doherty G, & Iyer A.K. (2016). Autophagy-Induced Apoptosis in Lung Cancer Cells by a Novel Digitoxin Analog. Journal of cellular physiology, 231(4), 817-828.
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