Plasmid miniprep kits

Manufactured by Metabion

Sourced in Germany

Plasmid-miniprep kits are designed for the isolation of plasmid DNA from bacterial cultures. They provide a simple and efficient method for purifying plasmid DNA from small-scale cultures.

Automatically generated - may contain errors

Lab products found in correlation

Gel extraction and pcr purification kits, by Qiagen (2 mentions) Ni nta agarose, by Macherey-Nagel (2 mentions) Porphyrins, by Frontier Scientific (2 mentions) Oligonucleotide primers, by Metabion (2 mentions) Restriction enzyme, by New England Biolabs (1 mentions) Dna polymerase, by New England Biolabs (1 mentions) Q5 site directed mutagenesis kit, by New England Biolabs (1 mentions)

2 protocols using plasmid miniprep kits

Cloning and Expression of Methanogenic Genes

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All chemicals and reagents were obtained from Sigma-Aldrich (Taufkirchen, Germany), Carl Roth (Karlsruhe, Germany), Gerbu (Heidelberg, Germany), Merck (Darmstadt, Germany). DNA polymerases, restriction enzymes, and PCR requisites were purchased from New England Biolabs (Frankfurt a.M., Germany). Oligonucleotide primers and plasmid-miniprep kits were purchased from Metabion (Martinsried, Germany). PCR purification and gel extraction kits were obtained from Qiagen (Hilden, Germany). Ni-NTA agarose was purchased from Macherey-Nagel (Düren, Germany). Porphyrins were purchased from Frontier Scientific (Newark, USA). The four E. coli codon-optimized genes Mbar_A1459, _A1460, _A1458, and _A1793 from M. barkeri were synthesized by Life Technologies GmbH (Darmstadt, Germany).

Kühner M., Haufschildt K., Neumann A., Storbeck S., Streif J, & Layer G. (2014). The Alternative Route to Heme in the Methanogenic Archaeon Methanosarcina barkeri. Archaea, 2014, 327637.

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Purification and Characterization of Mbar_A1458

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All chemicals and reagents were obtained from Sigma-Aldrich (Taufkirchen, Germany), Carl Roth (Karlsruhe, Germany), Gerbu (Heidelberg, Germany), Merck (Darmstadt, Germany). DNA polymerases, restriction enzymes, PCR requisites as well as the Q5 Site-Directed Mutagenesis Kit were purchased from New England Biolabs (Frankfurt a. M., Germany). Oligonucleotide primers and plasmid-miniprep Kits were purchased from Metabion (Martinsried, Germany). PCR purification and gel extraction Kits were obtained from Qiagen (Hilden, Germany). Ni-NTA agarose was purchased from Macherey-Nagel (Düren, Germany). Porphyrins were all purchased from Frontier Scientific (Logan, UT, USA) except of Zn-coproporphyrin III. The for E. coli codon-optimized gene Mbar_A1458 from M. barkeri was synthesized by Life Technologies GmbH (Darmstadt, Germany).

Kühner M., Schweyen P., Hoffmann M., Ramos J.V., Reijerse E.J., Lubitz W., Bröring M, & Layer G. (2016). The auxiliary [4Fe–4S] cluster of the Radical SAM heme synthase from Methanosarcina barkeri is involved in electron transfer †Electronic supplementary information (ESI) available: The cyclic voltammetry experiments, the binding assays with the substrate analogs, the determination of Kd values, the SAM cleavage assays with the substrate analogs, the decarboxylase activity assays with the substrate analogs and the synthesis and characterization of Zn-COPRO III. See DOI: 10.1039/c6sc01140c. Chemical Science, 7(7), 4633-4643.

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