T2 was obtained from Sigma-Aldrich (St. Louis, MO, USA) and dissolved in DMEM at 10 µM. Primary antibodies (rabbit anti-β-actin pAb (CST, Danvers, MA, USA) and mouse anti-IκBα mAb (Proteintech, Rosemont, IL, USA)) and secondary antibodies applied for Western blotting (goat anti-mouse IgG (H+L) (Promega, Madison, WI, USA) and goat anti-rabbit IgG (H+L) (Promega, Madison, WI, USA)) were purchased from commercial companies. Cyanine 5(Cy5)-conjugated goat anti-mouse IgG used in confocal microscopy was obtained from Gibco BRL life Technologies (New York City, NY, USA). Mouse monoclonal anti-cleaved-caspase-3 antibody and anti-cleaved-caspase-8 were purchased from Servicebio (Hubei, Wuhan, China). Rabbit polyclonal anti-Nrf2 antibody, anti-Gpx-1, mouse monoclonal anti-Bax antibody, and anti-Bcl-2 were purchased from Bioss (Beijing, China). In addition, the primary antibody mouse anti-PRV-gB mAb was a gift obtained from Dr. Ping Jiang (College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, China).
Goat anti mouse igg h l
Manufactured by Promega
Sourced in United States
Goat anti-mouse IgG (H+L) is a laboratory reagent that is used to detect the presence of mouse immunoglobulin G (IgG) in samples. It is a polyclonal antibody produced in goats and specific to the heavy and light chains of mouse IgG.
Automatically generated - may contain errors
Lab products found in correlation
Goat anti rabbit igg h l, by Promega (2 mentions)
Anti bcl 2, by Bioss Antibodies (1 mentions)
0.45 m pvdf membrane, by Merck Group (1 mentions)
Passive lysis buffer (plb), by Promega (1 mentions)
Non radioactive cytotoxicity assay kit, by Promega (1 mentions)
High pure pcr template preparation kit for genomic dna, by Roche (1 mentions)
O phenylenediamine dihydrochloride, by Merck Group (1 mentions)
Trypan blue stain solution, by Bio-Rad (1 mentions)
Proteinase k, by Roche (1 mentions)
Pc12 cells, by American Type Culture Collection (1 mentions)
3 protocols using goat anti mouse igg h l
1
Western Blot and Immunofluorescence Assay
2
Western Blot Analysis of ISKNV Infection
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Cells were cultured in 6-well plates. The infected cells were collected after infection 48 and 72 h and lysed in 200 μL of Passive Lysis Buffer (Promega, USA), and the protein concentration was determined using a BCA protein quantification kit (Genesand, China). Equal amounts of protein samples were separated via SDS-PAGE and transferred to a 0.45 µm PVDF membrane (Millipore, USA). The protein expression levels of ISKNV were measured by a mouse anti-isknvORF108L (an ISKNV viral structural protein) antibody (mAb2D8, from Dr. Chuanfu Dong). The antibody was also named anti-ISKNV-VP101L. To manifest equal protein sample loading, S. chuatsi GAPDH expression was measured using rabbit anti-GAPDH (Abways, China). Goat anti-mouse IgG (H + L) and goat anti-rabbit IgG (H + L) (Promega, USA) were used as the secondary antibodies. Finally, the images were acquired from Amersham Image 600 using a High-sig ECL Western blotting substrate kit (Tanon, China).
3
Apoptosis Mechanism in PC12 Cells
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PC12 cells were purchased from the American Type Culture Collection (USA and Canada). Dulbecco's modified Eagle medium (DMEM), ribonuclease A and o-phenylenediamine dihydro chloride (OPD) were obtained from Sigma-Aldrich (St. Louis, MO, USA). NP 2 EO was purchased from Tokyo Chemical Industry Corporation (Tokyo, Japan). Fetal bovine serum (FBS) was obtained from HyClone (Rockville, MD USA). Monoclonal antibody against cytochrome c, and polyclonal antibody against Bcl-2 were from Santa Cruz Biotechnology (Santa Cruz, CA, USA). Nonradioactive cytotoxicity assay kit and purified horseradish peroxidase conjugate of goat anti-mouse IgG (H+L) were obtained from Promega (Madison, WI, USA). Polyclonal antibody against Bax was provided by Calbiochem (San Diego, CA, USA). Anti-rabbit immunoglobulin peroxidase conjugate was obtained from Amersham Bioscience UK Limited (UK). Amersham TM ECL TM western blotting detection reagents were obtained from GE Healthcare UK Limited (UK). The High Pure PCR template Preparation Kit for genomic DNA and Proteinase K was obtained from Roche Diagnostics (Basel, Switzerland). Trypan blue stain solution (0.5%) was obtained from Bio-Rad (Hercules, CA, USA). The Cytochrome c Releasing Apoptosis Assay kit was from Medical and Biological Laboratories Co., Ltd (Japan). All other chemicals were analytical grade.
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