2 2 azinobis 3 ethylbenzothiazoline 6 sulphonate

Manufactured by Merck Group

Sourced in United States

2,2′-azinobis(3-ethylbenzothiazoline-6-sulphonate) is a water-soluble chromogenic compound used as a substrate in enzymatic assays. It is commonly employed in colorimetric detection and quantification procedures.

Automatically generated - may contain errors

Lab products found in correlation

Spelling variants of this product

2,2′-azinobis(3 (27 citations)

6 protocols using 2 2 azinobis 3 ethylbenzothiazoline 6 sulphonate

Assessing Antioxidant Potential of Açaí Fruit

Check if the same lab product or an alternative is used in the 5 most similar protocols

Freeze-dried and milled organic açaí fruit was obtained from “Healthy Foods”. Zein (Z 3625), 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2′-azinobis(3-ethylbenzothiazoline-6-sulphonate) (ABTS), Folin–Ciocalteu phenol reagent, anhydrous sodium carbonate, gallic acid (GA), ferric 2,4,6-tripyridyl-s-triazine (TPTZ) and 6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid (Trolox) were obtained from Sigma–Aldrich (Santiago, Chile). Lipase (L3126) and pancreatin (P1750) from porcine pancreas, pepsin (P6887) from porcine gastric mucosa, and porcine bile extract (B8631) were purchased from Sigma–Aldrich (Sigma–Aldrich S.A., USA). NaOH, HCl and different salts to prepare simulated digestion fluids (KCl, KH₂PO₄, NaHCO₃, NaCl, MgCl₂.(H₂O)₆, (NH₄)₂CO₃, and CaCl₂.(H₂O)₂) were purchased from Merck (Merck KGaA S.A., Darmstadt, Germany).

López de Dicastillo C., Piña C., Garrido L., Arancibia C, & Galotto M.J. (2019). Enhancing Thermal Stability and Bioaccesibility of Açaí Fruit Polyphenols through Electrohydrodynamic Encapsulation into Zein Electrosprayed Particles. Antioxidants, 8(10), 464.

+ Open protocol

+ Expand

Comparative Antioxidant and Tyrosinase Inhibition

Check if the same lab product or an alternative is used in the 5 most similar protocols

Tyrosinase from mushroom lyophilized powder (EC 1.14.18.1), hyaluronidase from bovine testes (EC 3.2.1.35), gallic acid, kojic acid, Trolox, l-ascorbic acid, l-tyrosine, l-DOPA, trifluoroacetic acid, Folin–Ciocalteu reagent, 2,4,6 tripyridyl-s-triazine (TPTZ), 2,2′-diphenyl-1-picrylhydrazyl-hydrate (DPPH), and 2,2’-azinobis 3-ethylbenzothiazoline-6-sulphonate (ABTS) were purchased from Sigma-Aldrich (St. Louis, MO, USA). Ferrous sulfate (FeSO₄), sodium carbonate (Na₂CO₃), sodium acetate (CH₃COONa), acetic acid (CH₃COOH), hydrochloric acid (HCl), potassium persulfate (K₂S₂O₈), sodium chloride (NaCl), monosodium phosphate (NaH₂PO₄), and disodium phosphate (Na₂HPO₄) were purchased from RCI Labscan Co., Ltd. (Bangkok, Thailand). Bovine serum albumin, AR grade ethanol, and HPLC grade acetonitrile were purchased from Merck (Darmstadt, Germany).

Chaiyana W., Sirithunyalug J., Somwongin S., Punyoyai C., Laothaweerungsawat N., Marsup P., Neimkhum W, & Yawootti A. (2020). Enhancement of the Antioxidant, Anti-Tyrosinase, and Anti-Hyaluronidase Activity of Morus alba L. Leaf Extract by Pulsed Electric Field Extraction. Molecules, 25(9), 2212.

+ Open protocol

+ Expand

Phytochemical Analysis and Antioxidant Activity

Check if the same lab product or an alternative is used in the 5 most similar protocols

Gallic acid, rutin, toluidine blue, DNS reagent, cephalexin, and ampicillin were purchased from Yuanye Biotechnology Co. (Shanghai, China). Acarbose, α-amylase, α-glucosidase, p-nitrophenyl-β-D-galactopyranoside (PNPG), 2,3,5-triphenyl tetrazolium chloride (TTC), 6-Hydroxy-2,5,7,8-tetramethylchromane-2-carboxylic acid (Trolox), Folin–Ciocalteu reagent, dimethyl sulfoxide (DMSO), 1,1-Diphenyl-2-picrylhydrazyl (DPPH), 2,2′-Azinobis-(3-ethylbenzothiazoline-6-sulphonate) (ABTS), and 2,4,6-tris(2-pyridyl)-s-triazine (TPTZ) were purchased from Sigma-Aldrich (St. Louis, MO, USA). Sodium hydroxide, hydrochloric acid, glacial acetic acid, ferric chloride, anhydrous sodium carbonate, potassium persulfate, sodium acetate, aluminum nitrate, sodium nitrite, and other regents were obtained from Bodi Chemical Co. (Tianjin, China). HPLC-grade formic acid, acetonitrile, and methanol were bought from CNW Technologies GmbH (Duesseldorf, Germany).

Jiang F., Li M., Huang L., Wang H., Bai Z., Niu L, & Zhang Y. (2024). Metabolite Profiling and Biological Activity Assessment of Paeonia ostii Anthers and Pollen Using UPLC-QTOF-MS. International Journal of Molecular Sciences, 25(10), 5462.

+ Open protocol

+ Expand

Antioxidant Evaluation Reagent Procurement

Check if the same lab product or an alternative is used in the 5 most similar protocols

Folin-Ciocalteu reagent, sodium carbonate anhydrous (Na₂CO₃), gallic acid, sodium nitrite solution (NaNO), sodiumhydroxyde (NaOH), aluminum chloride hexahydrate solution (AlCl₃, 6H₂O), iron (III) chloride anhydrous (FeCl₃), and catechin were purchased from Fluka (Buchs, Switzerland). 2,2-diphenyl-1-picrylhydrazyl-(DPPH), 2,6-di-tert-butyl-4-hydroxytoluene (BHT), Trolox (6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid), and 2,2’-azino-bis(3-ethylbenzothiazoline-6-sulphonate (ABTS) were purchased from Sigma-Aldrich (Murcia, spain), Tris (hydroxymethyl)-aminomethane (TRIS), and l-cysteine of analytical grade were obtained from Merck, Darmstadt, Germany. 5,5-dithiobis (2-nitrobenzoic acid) (DTNB) all of analytical grade were obtained from Fluka, Stenheim, Germany. Sodium dodecyl sulfate (SDS) was obtained from AppliChem GmbH, Darmstadt, Germany. Commercial mix was purchased from a supermarket (Murcia, spain).

Hajlaoui H., Arraouadi S., Mighri H., Chaaibia M., Gharsallah N., Ros G., Nieto G, & Kadri A. (2019). Phytochemical Constituents and Antioxidant Activity of Oudneya Africana L. Leaves Extracts: Evaluation Effects on Fatty Acids and Proteins Oxidation of Beef Burger during Refrigerated Storage. Antioxidants, 8(10), 442.

+ Open protocol

+ Expand

Cucumis metuliferus Antioxidant Extraction

Check if the same lab product or an alternative is used in the 5 most similar protocols

Cellulose acetate with Mn = 30,000 and 39.8 wt.% acetyl content (CA degree of substitution = 2.5 [42 (link)]) was supplied by Sigma-Aldrich (Santiago, Chile). A commercial corona-treated low-density polyethylene (LDPE) film was kindly supplied by EDELPA (Santiago, Chile). The Cucumis metuliferus fruits were obtained at a local market in Santiago de Chile. 2,2-azinobis(3-ethylbenzothiazoline-6-sulphonate) (ABTS), Folin Ciocalteu phenol reagent, anhydrous sodium carbonate, gallic acid and 6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid (Trolox) were purchased from Sigma Aldrich. Acetone (99.9% HPLC grade) and absolute ethanol (99.9% HPLC grade) were supplied by Merck (Santiago, Chile).

Arrieta M.P., Garrido L., Faba S., Guarda A., Galotto M.J, & López de Dicastillo C. (2020). Cucumis metuliferus Fruit Extract Loaded Acetate Cellulose Coatings for Antioxidant Active Packaging. Polymers, 12(6), 1248.

+ Open protocol

+ Expand

Enzymatic Hydrolysis of Dried SY

Check if the same lab product or an alternative is used in the 5 most similar protocols

Dried SY with a protein concentration of 41.96% was kindly provided by a beer factory from Ploiesti, Romania. Bromelain was provided by Carl Roth (Karlsruhe, Germany), Neutrase 5.0 BG by Novo Nordisk (Bagsværd, Denmark) and trypsin by Merck (Darmstadt, Germany), whereas o-phthaldialdehyde (OPA), N-acetyl-cysteine (NAC), sodium dodecyl sulfate (SDS), 2,2-diphenyl-1-picryl-hydrazyl (DPPH) and 2,2′-azinobis(3-ethyl-benzothiazoline-6-sulphonate) (ABTS) were purchased from Sigma Aldrich (Burlington, MA, USA). All other reagents were of analytical grade.

Dumitrașcu L., Lanciu Dorofte A., Grigore-Gurgu L, & Aprodu I. (2023). Proteases as Tools for Modulating the Antioxidant Activity and Functionality of the Spent Brewer’s Yeast Proteins. Molecules, 28(9), 3763.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!