Anti mouse igg1 pe

Bladder cancer cells were staining with anti-human CD155-PE mAb and mouse IgG1-PE isotype antibody (eBioscience) to detect the expression of CD155. To verify the successful coupling of CD155Bi-Ab, PUMC-91/ADM cells and ATCs were incubated with CD155Bi-Ab for 30 minutes, and then stained with anti-mouse IgG1-PE or anti-mouse IgG2a-PE. The combination of CD155 with OKT3 was used as the negative control. ATCs were incubated with anti-human CD3-FITC, anti-human CD4-PE, anti-human CD8-APC, and anti-human CD56-APC to examine the population of effector cells. To detect the expression of CD69 on ATCs, floating cells from bladder cancer cells and ATC co-cultures were incubated with anti-human CD69-PE and anti-human CD3-FITC. The anti-human CD3-FITC, anti-human CD69-PE, anti-human CD4-PE, anti-human CD8-APC, anti-human CD56-APC, anti-mouse IgG1-PE and anti-mouse IgG2a-PE secondary antibodies were from eBioscience. The cells were analyzed by the flow cytometer (CytoFlex, Beckman coulter) and data were processed using the accompanying software (CytExpert, Beckman coulter).