Glucose god pap

The following plasma measurements were performed: glucose (Glucose GOD-PAP, Roche Diagnostics, Mannheim, Germany, coefficient of variation 1.7%, range 241 mg/dL), total cholesterol (CHOD-PAP, Roche Diagnostics, Mannheim, Germany, coefficient of variation 4.0%, range 318 mg/dL), triglycerides (GPO-PAP, Roche Diagnostics, Mannheim, Germany, coefficient of variation 6.4%, range 117 mg/dL), high-density lipoprotein cholesterol (HDL-C) (HDL-C without sample pre-treatment, Roche Diagnostics, Mannheim, Germany, coefficient of variation 4.1%, range 74 mg/dL), HbA1c (Variant II, Bio-Rad Laboratories, Hercules, CA, USA), high-sensitivity C-reactive protein (CRP) (Cardiophase, Dade Behring, Marburg, Germany, coefficient of variation 6.7%, 13.8 g/L). Low-density lipoprotein cholesterol (LDL-C, range 280 mg/dL) was calculated by the Friedewald formula. Technicians, who were blinded to the statin treatment, performed all laboratorial analyses.

Cheek cell samples were taken in the clinic using two buccal swabs from the respective companies. The samples were sent by courier to the laboratory (Synlab Italia Srl, Monza, Italy). For DNA extraction swabs were added to 550 μl of sterile, nuclease-free H₂O and DNA extracted with QIASYMPHONY DSP DNA Mini kit eluted in 100 μl liquid. The genotype analysis was done with MassARRAY system with iPLEX chemistry (ex-Sequenom now called Agena) on a 384 chip. Primers and PCR conditions were designed with Agena Assay Design Suite (ADS) software. Through this process, genetic information (Table 1) was determined.
Fasting venous blood samples were taken at baseline, 24-weeks and 104-weeks to determine total cholesterol (TC), high density lipoprotein (HDL) cholesterol, and fasting blood glucose (FBG). TC and HDL concentrations were measured using an enzymatic colorimetric method (CHOL-CHOD-PAP, HDL Homogenic Enzymatic reaction, Roche Diagnostic, Germany). FBG was determined using an enzymatic kit (Glucose GOD-PAP, Roche Diagnostic, Germany). Weight and height were also measured, and body mass index (BMI) was calculated by dividing each subject’s weight (kg) by the square of their height (m).

Twelve-hour fasting plasma samples with EDTA were collected at admission and were immediately subjected to biochemical analyses in duplicates using an automatic chemical analyzer (Hitachi 917, Roche-Diagnostics). Glucose (Glucose GOD-PAP, Roche Diagnostics, Mannheim, USA), total cholesterol (CHOD-PAP, Roche Diagnostics, Mannheim, USA), triglycerides (GPO-PAP, Roche Diagnostics, Mannheim, USA), high-density lipoprotein cholesterol (HDL-C) (Roche Diagnostics, Mannheim, USA), high sensitivity C-reactive protein (CRP) (CardioPhase, Dade Behring, Marburg, USA), HbA1c (Variant II, Bio-Rad Laboratories, Hercules, CA, USA), apolipoprotein (apo) AI and B (Behring Nephelometer BNII, Dade Behring, Marburg, Germany) and plasma zinc (Atomic Absorption Spectrometry) were measured. Intra and interassay coefficients of variation for plasma zinc were 2.5% and 5.4%, respectively.