ND3 RNA probe (5′-UCCACCCCUUACGAGUGCGGCUUCGACCCUAUAUC-3′) and mutant ND3 RNA probe (5′-UCCACCCCUUACAAAUAAAGCUUCGACCCUAUAUC-3′) were purchased from GenScript (Piscataway, NJ). RNA-protein binding was performed in 20 μl reactions containing 1μM RNA probe. The binding conditions were 10 mM Tris at pH 7.5, 10 mM HEPES at pH 7.5, 20 mM KCl, 2 mM MgCl2, 1.5 mM DTT, 5% glycerol. After incubation at RT for 30 min, samples were run at 100 V on a pre-electrophoresed 6% polyacrylamide gel containing 0.5×TBE for 1 hour in ice water bath. Gel was stained by SYBR Green solution (Life Technologies, Grand Island, NY) for 20 min at RT. Then stained gel was rinsed with water for three times and visualized by EpiChemi II Darkroom (UVP, Upland, CA)
Nd3 rna probe
Manufactured by GenScript
The ND3 RNA probe is a laboratory tool used for the detection and analysis of the ND3 gene, which is involved in the mitochondrial respiratory chain. The probe is designed to specifically hybridize with the ND3 RNA sequence, allowing for its identification and quantification in various experimental settings. The core function of the ND3 RNA probe is to provide a reliable and accurate method for researchers to study the expression and regulation of the ND3 gene.
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2 protocols using nd3 rna probe
1
RNA-Protein Binding Assay with ND3 Probes
2
RNA-Protein Binding Assay with ND3 Probes
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ND3 RNA probe (5′-UCCACCCCUUACGAGUGCGGCUUCGACCCUAUAUC-3′) and mutant ND3 RNA probe (5′-UCCACCCCUUACAAAUAAAGCUUCGACCCUAUAUC-3′) were purchased from GenScript (Piscataway, NJ). RNA-protein binding was performed in 20 μl reactions containing 1μM RNA probe. The binding conditions were 10 mM Tris at pH 7.5, 10 mM HEPES at pH 7.5, 20 mM KCl, 2 mM MgCl2, 1.5 mM DTT, 5% glycerol. After incubation at RT for 30 min, samples were run at 100 V on a pre-electrophoresed 6% polyacrylamide gel containing 0.5×TBE for 1 hour in ice water bath. Gel was stained by SYBR Green solution (Life Technologies, Grand Island, NY) for 20 min at RT. Then stained gel was rinsed with water for three times and visualized by EpiChemi II Darkroom (UVP, Upland, CA)
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