K950x turbo evaporator

Manufactured by Emitech

Sourced in France

The K950X Turbo Evaporator is a laboratory equipment designed for the rapid evaporation of solvents from samples. It utilizes a high-speed turbine to create a controlled airflow, which accelerates the evaporation process. The K950X is capable of processing multiple samples simultaneously, making it suitable for a variety of applications that require efficient solvent removal.

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Lab products found in correlation

Hr fesem su 70 microscope, by Hitachi (1 mentions) Su 3800, by Bruker (1 mentions) Quantax compact 30, by Bruker (1 mentions) Integrating sphere, by PerkinElmer (1 mentions) Cm200 microscope, by Philips (1 mentions) Su 70 electron microscope, by Hitachi (1 mentions) Tga 50 system, by Shimadzu (1 mentions) Lambda 950 uv vis nir spectrophotometer, by PerkinElmer (1 mentions) Bx41 microscope, by Olympus (1 mentions)

7 protocols using k950x turbo evaporator

SEM Analysis of Biofilm Samples

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SEM analysis of biofilms were subjected to a pre-treatment where the synthetized biofilms were washed three times with water, at 2000 rpm for 10 min, in order to remove the cells which were not integrated and the culture media compounds. Subsequently, they were fixed using 2.5% glutaraldehyde in 0.1 M sodium phosphate buffer at pH 7.2 and kept overnight at a temperature of 4 °C. Afterwards, they were washed two times with a 0.1 M sodium phosphate buffer at pH 7.2, and then dehydrated with ethanol (30, 50, 70, 90 and 100% (v/v)) before they were freeze-dried. The samples were then preserved in a desiccator until analysis.
SEM images were acquired with a HR-FESEM SU-70 Hitachi microscope (Tokyo, Japan) operating at 4.0 kV, in the field emission mode. The films were deposited on a steel plate and coated with carbon before analysis using an EMITECH K950X Turbo Evaporator (Montigny-le-Bretonneux, France).

Nóbrega V., Faria M., Quintana A., Kaufmann M., Ferreira A, & Cordeiro N. (2019). From a Basic Microalga and an Acetic Acid Bacterium Cellulose Producer to a Living Symbiotic Biofilm. Materials, 12(14), 2275.

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ATR-FTIR Analysis of Thin Films

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ATR-FTIR spectra were obtained using a Perkin-Elmer (Waltham operating at 4.0 kV, in the field emission mode. The films were deposited on a steel plate and coated with carbon before analysis using an EMITECH K950X Turbo Evaporator (Montigny-le-Bretonneux, France).

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Analyzing Microstructure of Hollow Microchannels

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The morphology of
the hollow microchannels and their interconnection were analyzed in
a field emission gun scanning electron microscope (FESEM Hitachi SU-3800,
Japan) coupled with a Bruker Quantax compact 30 detector operated
in the secondary electrons mode at an acceleration voltage of 15 kV.
Prior to the analysis, the samples were dried with a gradient of ethanol
(10, 30, 50, 80, and 100%, for 30 min in each solution), dehydrated
overnight at RT, fixed on aluminum stubs by double-sided carbon conductive
adhesive tape, and sputtered coated with carbon (K950X Turbo Evaporator,
Emitech, U.S.).

Sousa V., Amaral A.J., Castanheira E.J., Marques I., Rodrigues J.M., Félix V., Borges J, & Mano J.F. (2023). Self-Supporting Hyaluronic Acid-Functionalized G-Quadruplex-Based Perfusable Multicomponent Hydrogels Embedded in Photo-Cross-Linkable Matrices for Bioapplications. Biomacromolecules, 24(7), 3380-3396.

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Comprehensive Thermal and Optical Characterization of Materials

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Thermogravimetric analysis (TGA) was performed using a Shimadzu TGA-50 system, in the temperature range from 25 to 800°C at a heating rate of 10°C min^–1, under a static atmosphere of air. Polarized optical microscopy (POM) images were obtained with an Olympus BX41 microscope, using crossed polarizers. Scanning Electronic Microscopy (SEM) images were registered with a Hitachi SU-70 electron microscope. The samples were attached to aluminum stubs using double-sided carbon adhesive tape or carbon glue. Pictures were obtained from the surface and cross-section areas. All the samples were sputter-coated with carbon through an EMITECH K950X Turbo Evaporator, at a single pulse, on an outgassing time of 30 s and an evaporating time of 2 s. Transmission electron microscopy (TEM) images were obtained by a Philips CM200 microscope with an accelerating potential of 100 keV. TEM samples were prepared using a method described by Giasson et al. (1988) (link).
UV-Vis reflectance spectroscopy was carried out using a Perkin-Elmer Lambda 950 UV/Vis/NIR spectrophotometer and a Spectralon integrating sphere (Ø = 150 mm). The freestanding film surface was placed perpendicularly to the incident beam and the spectra were acquired as a function of the incident angle (15° < θ < 60°) with 5° steps, as illustrated in Supplementary Scheme 1.

Santos M.V., Maturi F.E., Pecoraro É., Barud H.S., Lima L.R., Ferreira R.A., Carlos L.D, & Ribeiro S.J. (2021). Cellulose Based Photonic Materials Displaying Direction Modulated Photoluminescence. Frontiers in Bioengineering and Biotechnology, 9, 617328.

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Biopolymer Characterization via SEM

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Dry BC biopolymers (OD-BC and FD-BC) were coated with a thin layer of carbon using EMITECH K950X Turbo Evaporator and deposited on a steel plate. SEM micrographs were obtained using the HR-FESEM SU-70 Hitachi Scanning Electron Microscopy equipment, operating with a 5 kV beam, at a 15.6 mm working distance, in the field emission mode. Images were collected at several magnifications, from x500 to x30 000.

Faria M., Cunha C., Gomes M., Mendonça I., Kaufmann M., Ferreira A, & Cordeiro N. (2022). Bacterial cellulose biopolymers: The sustainable solution to water-polluting microplastics. Water research, 222.

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SEM Imaging of Microparticle Surfaces

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SEM micrographs ( Fig. 1 ) of the MPs surface were obtained using an HR-FESEM SU-70 Hitachi Scanning Electron Microscopy equipment, operating with a 5 kV beam, at a 15.6 mm working distance, in the field emission mode. Images were collected at x100 magnification. Prior to analysis, the samples were coated with a thin layer of carbon using EMITECH K950X Turbo Evaporator and deposited on a steel plate.

Cunha C., Lopes J., Paulo J., Faria M., Kaufmann M., Nogueira N., Ferreira A, & Cordeiro N. (2020). The effect of microplastics pollution in microalgal biomass production: A biochemical study. Water research, 186.

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SEM Analysis of Hetero-Aggregates

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Before SEM analysis, the formed hetero-aggregates were washed three times with water and centrifuged at 2000 rpm, for 10 min, to remove any floating cells and culture medium compounds. Then, the hetero-aggregates were fixed using 2.5% (w/v) glutaraldehyde in 0.1 M sodium phosphate buffer, at pH 7.2, and stored overnight at 4 C. After, the samples were washed two times with 0.1 M sodium phosphate buffer, at pH 7.2, and then serially dehydrated with ethanol (30%, 50%, 70%, 90% and dry 100%, v/v, ethanol/water) before freeze-drying. Samples were then preserved in a desiccator until analysis. Scanning electron micrographs of the surface samples were obtained by Scanning Electron Microscopy (SEM), with an HR-FESEM SU-70 Hitachi equipment operating at 4 kV, in the field emission mode. Samples were deposited on a steel plate and coated with carbon before analysis (EMITECH K950X Turbo Evaporator).

Cunha C., Faria M., Nogueira N., Ferreira A, & Cordeiro N. (2019). Marine vs freshwater microalgae exopolymers as biosolutions to microplastics pollution. Environmental pollution (Barking, Essex : 1987), 249.

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