Dialysis tube 3 500 mwco

Manufactured by Thermo Fisher Scientific

Dialysis tube (3,500 MWCO) is a laboratory equipment used for the separation and purification of molecules based on their molecular weight. The 3,500 MWCO (Molecular Weight Cut-Off) indicates that the tube is designed to retain molecules with a molecular weight greater than 3,500 Daltons, while allowing smaller molecules to pass through.

Automatically generated - may contain errors

Lab products found in correlation

Sterile cell strainer, by Thermo Fisher Scientific (3 mentions) Tube magnetic stand, by Thermo Fisher Scientific (2 mentions) Plate magnet, by Merck Group (2 mentions) Ultrapure distilled water, by Thermo Fisher Scientific (2 mentions) D glucosamine hydrochloride, by Thermo Fisher Scientific (2 mentions) D glucosamine hydrochloride, by Merck Group (2 mentions) Streptavidin magnetic beads, by New England Biolabs (1 mentions) 9.3 m libr, by Merck Group (1 mentions) Ultrapure water, by Thermo Fisher Scientific (1 mentions) Digalacturonic acid, by Merck Group (1 mentions)

Spelling variants of this product

Dialysis tube (14 citations)

7 protocols using dialysis tube 3 500 mwco

Magnetic Bead-Based Protein Enrichment

Check if the same lab product or an alternative is used in the 5 most similar protocols

A 20 μl aliquot of streptavidin magnetic beads (New England Biolabs) was washed with once with 200 μl Buffer A (10 mM Tris pH 8.0, 50 mM NaCl, 10 mM CaCl₂, 0.01% Tween 20) and resuspended in 50 μl of Buffer A containing 500 ng of biotinylated-His-HpaII. After pipette mixing to allow the His-HpaII to bind to the beads, the His-HpaII-beads (“HpaII-beads” for simplicity) were washed again with Buffer A. Enrichments were performed either in 1.7 ml microcentrifuge tubes or in a 96-well plate. DNA samples suspended in 50 μl of Buffer B (10 mM Tris pH 8.0, 250 mM NaCl, 10 mM CaCl₂, 0.01% Tween 20) were added to HpaII-beads and mixed for the indicated time. Magnetic beads were separated using either a tube magnetic stand (Life Technologies) or a plate magnet (Millipore, Billerica, MA). The beads were washed once with 200 μl Buffer A, and then resuspended in 50 μl of Buffer B for qPCR analysis.
For gel analysis and next-generation library preparation, the DNA was eluted from beads by incubation with 50 μl 5 M guanidinium thiocyanate at room temperature for 5 minutes. The eluent was transferred to a 3,500 MWCO dialysis tube (Thermo Scientific, Waltham, MA) and dialyzed against distilled water for 1 hour at room temperature.

Liu G., Weston C.Q., Pham L.K., Waltz S., Barnes H., King P., Sphar D., Yamamoto R.T, & Forsyth R.A. (2016). Epigenetic Segregation of Microbial Genomes from Complex Samples Using Restriction Endonucleases HpaII and McrB. PLoS ONE, 11(1), e0146064.

+ Open protocol

+ Expand

DNA Sample Preparation and Purification

Check if the same lab product or an alternative is used in the 5 most similar protocols

DNA samples were prepared in Binding Buffer. The assay was performed either in 1.7 ml microcentrifuge tubes or in a 96-well plate. 50 µl DNA samples were added to the DpnI coated beads. The beads were mixed by end-over-end rotation or on a plate shaker for 5 minutes to 1 hour. Magnetic beads were separated using either a tube magnetic stand (Life Technologies) or a plate magnet (Millipore, Billerica, MA). The beads were washed once with Wash Buffer (10 mM Tris pH 7.9, 500 mM NaCl, 10 mM CaCl₂, 0.1% Tween 20) followed by one Binding Buffer wash. Beads were resuspended in 50 µl of Binding Buffer for qPCR analysis.
For gel analysis and next-generation library preparation, the DNA was eluted from beads by incubation with 50 µl 5 M guanidinium thiocyanate at room temperature for 5 minutes. The eluent was transferred to a 3500 MWCO dialysis tube (Thermo Scientific, Waltham, MA) and dialyzed against distilled water for 1 hour at room temperature.

Barnes H.E., Liu G., Weston C.Q., King P., Pham L.K., Waltz S., Helzer K.T., Day L., Sphar D., Yamamoto R.T, & Forsyth R.A. (2014). Selective Microbial Genomic DNA Isolation Using Restriction Endonucleases. PLoS ONE, 9(10), e109061.

+ Open protocol

+ Expand

Silk Fibroin Extraction and Purification

Check if the same lab product or an alternative is used in the 5 most similar protocols

Silk fibroin (SF) solutions were prepared using our previously reported protocol.^{[67 (link)]} Briefly, 5 grams of B. mori silkworm cocoons were cut into small pieces and extracted in 2 L of 0.02 M Na₂CO₃ solution (Sigma-Aldrich, St. Louis, MO) in a glass beaker for 30 and 120 minutes separately to remove the sericin protein coating. Degummed fibers were collected and rinsed with deionized water (DI) in a 4L bucket three times for 20 minutes, followed by air-drying in a fume hood overnight. The degummed fibers were solubilized in 9.3 M LiBr (Sigma-Aldrich, St. Louis, MO), the following day, in a pre-heated oven at 60°C for 4h. After 4h, a clear light brown color SF solution was obtained which was then dialyzed against 4L of DI water with six water changes after 1, 2, 4, 24, 36, and 48 h. The dialysis was performed with a dialysis tube (3,500 MWCO, Thermo Scientific, Rockford, IL). After 48h, Centrifugation of the dialyzed silk solution was performed twice (9,000 RPM, 20 min, 4°C) to remove insoluble white/brown aggregates. The concentration of the silk solution was determined by drying a known mass of the silk solution in a weighing boat in an oven at 60°C overnight and assessing the mass of the remaining solid film.

Sahoo J.K., Hasturk O., Choi J., Montero M.M., Descoteaux M.L., Laubach I.A, & Kaplan D.L. (2021). Sugar functionalization of silks with pathway-controlled substitution and properties. Advanced biology, 5(7), e2100388.

+ Open protocol

+ Expand

Silk-Glucosamine Conjugation for Biomaterials

Check if the same lab product or an alternative is used in the 5 most similar protocols

The carboxylic acids of as prepared silk solutions of different MW distribution (157 and 79 kDa) (Asp and Glu residues) were conjugated with amines of D-(+)-glucosamine hydrochloride (Sigma-Aldrich, St. Louis, MO) by carbodiimide coupling in presence of EDC and NHS. Briefly, 2 wt% of the silk solution was dissolved in 0.1M MES buffer at pH 6. D-(+)-glucosamine hydrochloride (10X) was weighed and pre-dissolved in Ultrapure™ distilled water (Thermo-Fisher Scientific, Waltham, MA) and added to the silk solution. The pH was adjusted to 6 by dropwise addition of 1M NaOH. EDC (10X) and NHS (10X) were added to the reaction mixture at pH 6. Ultrapure™ distilled water was added to the reaction to maintain the final MES buffer concentration to 0.05M. The reaction was stirred at RT for 18h. After 18h, the reaction mixture was filtered in a sterile cell strainer with 40 μm mesh size (Thermo Scientific, Rockford, IL) to remove any aggregates before starting dialysis against DI water for 72h (water changes at 1h, 2h, 4h, 24h, 48h, and 72h). The dialysis was performed with a dialysis tube (3,500 MWCO, Thermo Scientific, Rockford, IL). After dialysis, the solutions were frozen at −80 °C overnight followed by lyophilizing for 72h. The lyophilized powders were stored at 4 °C until further use.

+ Open protocol

+ Expand

Silk-Glucosamine Conjugation Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols

The prepared tyrosine carboxylated silk solutions (SF(Y)-COOH) were conjugated with amines of D-(+)-glucosamine hydrochloride (Sigma-Aldrich, St. Louis, MO) by carbodiimide coupling in the presence of EDC and NHS. Briefly, 2 wt% of the silk solution was dissolved in 0.1M MES buffer at pH 6. D-(+)-glucosamine hydrochloride (10X) was weighed and pre-dissolved in ultrapure water (Thermo-Fisher Scientific, Waltham, MA) and added to the silk solution. The pH was adjusted to 6 by dropwise addition of 1M NaOH. EDC (10X) and NHS (10X) were added to the reaction mixture at pH 6. ultrapure water was added to the reaction to maintain the final MES buffer concentration to 0.05M. The reaction was stirred at RT for 18h. The aggregates were filtered after the reaction, in a sterile cell strainer with 40 μm mesh size (Thermo Scientific, Rockford, IL) before starting dialysis against DI water for 72h (water changes at 1h, 2h, 4h, 24h, 48h, 72h). The dialysis was performed with a dialysis tube (3,500 MWCO, Thermo Scientific, Rockford, IL). After dialysis, the solutions were frozen at −80 °C overnight followed by lyophilizing for 72h. The lyophilized powders were stored at 4 °C until further use.

+ Open protocol

+ Expand

Silk Fibroin Amine Conjugation for Biomaterial Applications

Check if the same lab product or an alternative is used in the 5 most similar protocols

The carboxylated silk solutions (SF(S)-COOH) of 157 kDa MW SF were conjugated with amines of ethylene diamine (EDA) hydrochloride (Sigma-Aldrich, St. Louis, MO) by carbodiimide coupling in the presence of EDC and NHS (Sigma-Aldrich, St. Louis, MO). Briefly, 2 wt% of the silk solution was dissolved in 0.1M MES (2-(N-morpholino) ethanesulfonic acid) buffer at pH 6. EDA (10X) was weighed and pre-dissolved in Ultrapure™ distilled water (Thermo-Fisher Scientific, Waltham, MA) and added to the silk solution. The pH was adjusted to 6 by dropwise addition of freshly prepared 1M sodium hydroxide (NaOH) solution. EDC (10X) and NHS (10X) were added to the reaction mixture at pH 6. The final MES buffer concentration of the reaction mixture was adjusted to 0.05M by addition of Ultrapure™ distilled water. The reaction was stirred at RT for 18h. After the reaction got over, the aggregates were filtered in a sterile cell strainer with 40 μm mesh size (Thermo Scientific, Rockford, IL) and dialyzed against DI water for 72h with six water changes (1h, 2h, 4h, 24h, 48h, and 72h). The dialysis was performed with a dialysis tube (3,500 MWCO, Thermo Scientific, Rockford, IL). After dialysis, the solutions were frozen at −80 °C overnight followed by lyophilizing for 72h. The lyophilized powders were stored at 4 °C until further use.

+ Open protocol

+ Expand

Silk-Digalacturonic Acid Conjugation

Check if the same lab product or an alternative is used in the 5 most similar protocols

The EDA conjugated silk solutions (SF(S)-EDA) of 157 kDa MW SF were carbodiimide coupled with the carboxylic acids of digalacturonic acid (Sigma-Aldrich, St. Louis, MO) in the presence of EDC and NHS (Sigma-Aldrich, St. Louis, MO). Briefly, 2 wt% of the SF(S)-EDA solution was dissolved in 0.1M MES buffer at pH 6. digalacturonic acid (10X) was weighed and pre-dissolved in Ultrapure™ distilled water and added to the SF(S)-EDA solution. The pH was adjusted to 6 by dropwise addition of freshly prepared 1M NaOH solution. EDC (10X) and NHS (10X) were added to the reaction mixture at pH 6. The final MES buffer concentration of the reaction mixture was adjusted to 0.05M by addition of Ultrapure™ distilled water. The reaction was stirred at RT for 18h. After the reaction got over, the reaction solution was filtered in a sterile cell strainer with 40 μm mesh size (Thermo Scientific, Rockford, IL) and dialyzed against DI water for 72h with six water changes (1h, 2h, 4h, 24h, 48h, and 72h). The dialysis was performed with a dialysis tube (3,500 MWCO, Thermo Scientific, Rockford, IL). After dialysis, the solutions were frozen at −80°C overnight followed by lyophilizing for 72h. The lyophilized powders were stored at 4°C until further use.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!