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1 3 dimethylaminopropyl 3 ethylcarbodiimide hydrochloride

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1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride is a water-soluble carbodiimide compound used as an activating reagent in chemical reactions. It is commonly used in the coupling of carboxyl groups with primary amines to form amide bonds.

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Lab products found in correlation

N hydroxysuccinimide, by Maclin Biochemical Technology (3 mentions) N n methylenebisacrylamide mba, by Tianjin Kermel (3 mentions) Ferric chloride, by Merck Group (1 mentions) Dopamine hydrochloride, by J&K Scientific (1 mentions) Sodium periodate naio4, by Merck Group (1 mentions) Anhydrous ferric chloride, by Merck Group (1 mentions) Milli q plus 185 water purification system, by Merck Group (1 mentions) Vitamin c l ascorbic acid, by Merck Group (1 mentions) Ethylene glycol, by Merck Group (1 mentions) Octadecylamine, by Adamas (1 mentions) Carboxymethyl chitosan, by Maclin Biochemical Technology (1 mentions) Oxymatrine, by Maclin Biochemical Technology (1 mentions) N hydroxysuccinimide, by Adamas (1 mentions) Rhodamine b, by Maclin Biochemical Technology (1 mentions) Succinic anhydride, by Maclin Biochemical Technology (1 mentions) Cisplatin, by Maclin Biochemical Technology (1 mentions) Triethylamine, by Maclin Biochemical Technology (1 mentions) Bovine serum albumin (bsa), by Maclin Biochemical Technology (1 mentions) Tetrahydrofuran, by Maclin Biochemical Technology (1 mentions) Ethylenediamine, by Maclin Biochemical Technology (1 mentions)

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1-(3-Dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride (EDC) (7 citations)

9 protocols using 1 3 dimethylaminopropyl 3 ethylcarbodiimide hydrochloride

1

Synthesis of SA-DA-TAA Hydrogel

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Sodium alginate (SA) was obtained from Aladdin. Dopamine hydrochloride (DA) and 3-thienylacetic acid (TAA) was obtained from J&K. 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride (EDC), N-hydroxysuccinyl imine (NHS) and carboxymethyl chitosan (CMC) were obtained from Macklin. Sodium periodate (NaIO4) and Ferric chloride were obtained from Sigma-Aldrich. The reagents were analytically pure and not further purified.
Qiao L., Liang Y., Chen J., Huang Y., Alsareii S.A., Alamri A.M., Harraz F.A, & Guo B. (2023). Antibacterial conductive self-healing hydrogel wound dressing with dual dynamic bonds promotes infected wound healing. Bioactive Materials, 30, 129-141.
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2

Fabrication and Characterization of Multifunctional Fluorescent Nanoprobes

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Ethylene glycol (EG), sodium acetate (NaAc), anhydrous ferric chloride (FeCl3), l‐ascorbic acid (Vitamin C), 1,1′‐dioctadecyl‐3,3,3′,3′‐tetramethylindocarbocyyanine perchlorate (Dil), and Hochest dye were purchased from Sigma‐Aldrich. Sodium hydroxide (NaOH), tetraethoxysilane (TEOS), ammonium hydroxide (NH3·H2O), glycerol triacetate, poly(4‐styrenesulfonic acid‐co‐maleic acid) sodium salt (PSSMA, Mw ≈ 20 000, SS:MA = 1:1), silicone oil (50cs), (3‐aminopropyl)triethoxysilane (APTES), 2‐morpholinoethanesulfonic acid (MES), N‐hydroxysuccinimide (NHS), and 1‐(3‐dimethylaminopropyl)‐3‐ethylcarbodiimide hydrochloride (EDC) were obtained from Shanghai Macklin Biochemical Co. Ltd. All DNA aptamers were synthesized by Sangon Biotech Co., Ltd. (Shanghai, China) and purified by ProStar HPLC (Varian) with a C18 column. NIH‐3T3 cells were purchased from Cell Bank of the Chinese Academy of Sciences (Shanghai, China). A549 and HepG2 cells were bought from Procell Life Science & Technology Co., Ltd. (Wuhan, China). The clinical serum samples of healthy people and patients were collected from the second Affiliated Hospital of Nanjing Medical University of China by standard venipuncture (IRB protocol number [2020]‐KY‐003). Distilled water used in all experiments was purified by a Milli‐Q Plus 185 water purification system (Millipore, Beford, MA) with the resistivity higher than 18 MΩ cm.
Chen H., Miao S., Zhao Y., Luo Z, & Shang L. (2023). Rotary Structural Color Spindles from Droplet Confined Magnetic Self‐Assembly. Advanced Science, 10(8), 2207270.
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3

Targeted Delivery of Oxymatrine via SPEG-CMCS Nanoparticles

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Oxymatrine (OMT), polyoxyethylene stearate (SPEG), rhodamine B (RB), 1-(3-Dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride (EDCI) and carboxymethyl chitosan (CMCS, N-linked, carboxylation degree 80%) were offered by Macklin Biochemical Co., Ltd. (Shanghai, China), respectively. Octadecylamine (ODA) and N-Hydroxysuccinimide (NHS) were supplied by Adamas-beta (Shanghai, China). Soybean phospholipid (SPC) was supplied by A.V.T Pharmaceutical Co., Ltd. (Shanghai, China). Cholesterol (Chol) was purchased from VetecTM reagent grade (Shanghai, China). Porcine pancreatic elastase (PPE) was purchased from Macklin Biochemical Co., Ltd. (Shanghai, China).
Peng J., Cai Z., Wang Q., Zhou J., Xu J., Pan D., Chen T., Zhang G., Tao L., Chen Y, & Shen X. (2022). Carboxymethyl Chitosan Modified Oxymatrine Liposomes for the Alleviation of Emphysema in Mice via Pulmonary Administration. Molecules, 27(11), 3610.
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4

Cisplatin-based Conjugate for Hepatic Cancer Treatment

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Triethylamine (TEA) and N, N-dimethylformamide (DMF) were dried over calcium hydride before use. Cisplatin (99%), N‑hydroxy succinimide (NHS, 98%), 2-amino-1,3-propanediol (98%), ethyl trifluoroacetate (99%), 1-(3-dimethylaminopropyl)−3-ethylcarbodiimide hydrochloride (EDC, 98%), succinic anhydride (SA, 99%), demethylcantharidin (DMC, 99.5%), and trinitrobenzene sulfonic acid (TNBSA, 98%) were purchased from Macklin (Shanghai, China). Human and murine hepatic cancer cell lines (HepG2 (passage number: 67) and H22 (passage number: 49)) were acquired from iCell Bioscience Inc (Shanghai, China). Male ICR and Balb/c nude mice (18–22 g) were obtained from Cyagen Biotechnology Co., Ltd (Jiangsu, China), and the care and use of animals followed the guidance of Anhui University Laboratory Animal Ethics and Management Committee (IACUC(AHU)−2022–013).
Wang S., Song Y., Ma J., Chen X., Guan Y., Peng H., Yan G, & Tang R. (2022). Dynamic crosslinked polymeric nano-prodrugs for highly selective synergistic chemotherapy. Asian Journal of Pharmaceutical Sciences, 17(6), 880-891.
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5

Synthesis of Berberine-Loaded Nanoparticles

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SA was purchased from Sinopharm Chemical
Regent Co., Ltd. (Shanghai, China). 1-(3-Dimethylaminopropyl)-3-ethylcarbodiimide
hydrochloride (EDC·HCl) and N-hydroxysuccinimide
(NHS) were obtained from Shanghai Macklin Biochemical Co., Ltd. (Shanghai,
China). Glu was supplied by Shanghai Aladdin Bio-Chem Technology Co.,
Ltd. (Shanghai, China). NIPAM was provided by Sann Chemical Technology
Co., Ltd. (Shanghai, China). N,N′-Methylenebisacrylamide (MBA) was purchased from Tianjin
Kermel Chemical Reagent Co., Ltd. (Tianjin, China). Ammonium persulfate
(APS) was obtained from Tianjin Damao Chemical Reagent Factory (Tianjin,
China). Berberine hydrochloride was purchased from Qilu Pharmaceutical
Co., Ltd. (Jinan, China).
Chang S., Qin D., Yan R., Zhang M., Sui B., Xu H., Zheng Z., Hou X., Wang Y, & Qi C. (2021). Temperature and pH Dual Responsive Nanogels of Modified Sodium Alginate and NIPAM for Berberine Loading and Release. ACS Omega, 6(2), 1119-1128.
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6

Synthesis of DOX-Loaded Gold Nanoparticles

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All glassware and stirring magnets were washed with aqua regia (HCl : HNO3 volume ratio = 3 : 1) and rinsed with ultrapure water thoroughly. Gold(iii) chloride trihydrate (HAuCl4·3H2O, Macklin, China), bovine serum albumin (BSA, Macklin, China), sodium hydroxide (NaOH, Macklin, China), ethanol (Macklin, China), 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride (EDC, Macklin, China), ethylenediamine (EDA, Macklin, China), doxorubicin hydrochloride (DOX·HCl, Macklin, China), triethylamine (TEA, Macklin, China), dimethyl sulfoxide (DMSO, Macklin, China) and tetrahydrofuran (THF, Macklin, China) were used as received.
Yang J., Zhang L., Zhou Q., Chen F., Stenzel M., Gao F., Liu C., Yuan H., Li H, & Jiang Y. (2021). Self-assembled anionic and cationic Au nanoparticles with Au nanoclusters for the exploration of different biological responsiveness in cancer therapy. Nanoscale Advances, 3(10), 2812-2821.
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7

Resveratrol-Loaded Hydrogel Synthesis

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BX was isolated from bagasse (self-extracted). Emulsifier OP-10, hydrochloric acid and sodium hydroxide were acquired from Xilong Chemical Factory (Shantou, China). Resveratrol was obtained from Genye Biotechnology Company limited (Shanghai, China). 3-Carboxyphenylboronic acid, vinyl dimethacrylate, 4-dimethylaminopyridine, 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride, ammonium persulfate (APS), N, N-dimethylformamide (DMF) and N, N-methylene acrylamide were obtained from Macklin (Shanghai, China). Ethanol and dimethyl sulfoxide (DMSO) were purchased from Luoyang Chemical Reagent Factory (Luoyang, China).
Zhao B., Li H., Su Y., Tian K., Zou Z, & Wang W. (2022). Synthesis and Anticancer Activity of Bagasse Xylan/Resveratrol Graft-Esterified Composite Nanoderivative. Materials, 15(15), 5166.
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8

Synthesis and Characterization of Coumarin-6 Labeled Soy Lecithin-Cholesterol Nanoparticles

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Soy lecithin (CS-95, SY-SO-220401) and cholesterol (C11884385) were generously provided by AVT Pharmaceutical Tech Co., Ltd. (Shanghai, China). Arctigenin (>98%; MUST-21062310) was purchased from Chengdu Mansite Pharmaceutical Co., Ltd. (Chengdu, China). Sialic acid (SA; H24A9W59567) was purchased from Yuanye Bio-Technology Co., Ltd. (Shanghai, China). N-hydroxysuccinimide (NHS, >98%; C12583367), 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride (EDC·HCl, >98%; C12373846), and octadecylamine (ODA; F2207274) were supplied by Macklin Biochemical Co., Ltd. (Shanghai, China), Coumarin-6 (C6; J1916037) was purchased from Aladdin Reagent (Shanghai, China). 4′,6-Diamidino-2-phenylindole (DAPI; 20220922) was purchased from Solarbio (Beijing, China). Cell Counting Kit-8 (CCK-8; MA0128-4-Oct-19H1) was purchased from Meilun Biotech Co., Ltd. (Dalian, China). DMEM (8122464) was purchased from Gibco Corp. (Carlsbad, CA, USA). Fetal bovine serum (2144324) was purchased from Biological Industries (Kibbutz Beit Haemek, Israel). Other chemicals were of analytical reagent or guaranteed reagent grade and were used without additional purification.
Liu S., He Y., Feng M., Huang Y., Wu W, & Wang J. (2024). Targeted Delivery of Arctigenin Using Sialic Acid Conjugate-Modified Liposomes for the Treatment of Breast Cancer. Molecules, 29(1), 278.
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9

Multifunctional Nanoparticle Synthesis and Evaluation

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All chemicals and reagents were of chemical grade and used without further purification. Chloroauric acid hydrated (HAuCl4), sodium citrate, poly (allylamine hydrochloride) (PAH, Mw 15,000, 98%), 1-(3-Dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride (EDC, 98.5%) and N-Hydroxysuccinimide (NHS, 98%) were purchased from Macklin (Shanghai, China). Fingolimod hydrochloride (FTY720, 98%), tetraphenylethylene (TPE, 98%), 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC, 99%), cholesteryl hemisuccinate (CHEMS, 97%), DSPE PEG Maleimide (DSPE-PEG-Mal, MW 2000 ​Da), DSPE PEG Acid (DSPE-PEG-COOH, MW 2000 ​Da), ethylene imine polymer (PEI, MW 1800, 99%), 2,3-dimethylmaleic anhydride (DMA, 98%) were obtained from Aladdin Reagent Co. Ltd (Shanghai, China). Dulbecco's modified eagle's medium (DMEM), trypsin-EDTA, and fetal bovine serum (FBS) were purchased from Gibco-BRL (Burlington, Canada). Cell counting Kit 8 (CCK8), calcein-acetylmethoxylate (calcein-AM), propidium iodide (PI), 4% paraformaldehyde, 0.1% Triton-X-100, 4,6-diamidino-2-phenylindole (DAPI) were purchased from Beyotime Biotechnology (Shanghai, China).
Chen T., Tam N., Mao Y., Sun C., Wang Z., Hou Y., Xia W., Yu J, & Wu L. (2022). A multi-hit therapeutic nanoplatform for hepatocellular carcinoma: Dual stimuli-responsive drug release, dual-modal imaging, and in situ oxygen supply to enhance synergistic therapy. Materials Today Bio, 16, 100338.
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