Rabbit anti gfap

The following primary antibodies were used throughout this study: rat anti-mouse CD11b (1:400, Abcam), rabbit anti-F-actin (1:1000, Abcam), rabbit anti-COX-2 (1:1000, Abcam), rabbit anti-IL-1β (1:200, Abcam), rabbit anti-GFAP (1:5000, Neuromics), rabbit anti-Iba-1 (1:1000, Wako), goat anti-Iba-1 (1:500, Wako), rabbit anti-AKT (1:1000, Santa Cruz), rabbit anti-p-AKT (Ser473, Thr308) (1:1000, Cell Signaling), rabbit anti-ERK (1:1000, Santa Cruz), rabbit anti-p-ERK (Thr42/44) (1:1000, Cell Signaling), rabbit anti-STAT3 (1:1000, Cell Signaling), rabbit anti-p-STAT3 (Ser727, Abcam), mouse anti-PCNA (1:1000, Santa Cruz), rabbit anti-D2R (1:1000, Abcam), and rabbit anti-D1R (1:1000, Millipore) antibodies. We used the following small molecules: D1R antagonists (LE300, 10 μM, Sigma-Aldrich; SCH23390, 30 μM, Tocris), D1R agonist (A77636 hydrochloride, 10 nM, Tocris), D2R antagonist (eticlopride hydrochloride, 100 nM, Sigma-Aldrich), a STAT3 inhibitor (S3I-201, 50 μM, Sigma-Aldrich), and an ERK inhibitor (PD98059, 10 μM, Millipore).

Chemicals were purchased from Sigma–Aldrich Company (St. Louis, MO, USA) unless otherwise indicated. The HDAC inhibitor trichostatin A (TSA) (ab120850) and HDAC6 inhibitor tubastatin A (ab141415) were purchased from Abcam (Cambridge, MA, USA). The primary antibodies used were rabbit anti-HDAC6 (cat. 7558, Cell Signaling (Danvers, MA, USA), 1:1000 for WB, 1:200 for staining), rabbit anti-GAPDH (cat. 5174, Cell Signaling, 1:10000 for WB), rabbit anti-Ac-K (cat. 9441, Cell Signaling, 1:1000 for WB), rabbit anti-Myc (cat. PA9064, Abmart, 1:1000 for WB), rabbit anti-Flag (cat. F7425, Sigma, 1:2000 for WB), mouse anti-NeuN (cat. MO22122, Neuromics (Edina, MN, USA), 1:1000 for staining), rabbit anti-GFAP (cat. Z0334, Dako (Kyoto, Japan), 1:1000 for staining), rabbit anti-Iba1 (cat. Ab5076, Abcam, 1:500 for staining), rabbit anti-γH2AX (cat. 9718, Cell Signaling, 1:1000 for WB, 1:500 for staining), and MIF (cat. Ab187064, Abcam, 1:1000 for WB, 1:500 for staining). The MIF K78 acetylation antibody was generated by ABclonal Biotechnology (Shanghai, China).