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Tecnai g2 12 transmission electron microscope

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The Tecnai G2 12 is a transmission electron microscope (TEM) manufactured by Thermo Fisher Scientific. It is designed to provide high-resolution imaging and analysis of materials at the atomic scale. The Tecnai G2 12 TEM uses a high-energy electron beam to interact with a thin sample, generating an image that can be used to study the structure and composition of a wide range of materials.

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Lab products found in correlation

Em uc6 ultramicrotome, by Leica (1 mentions) Quorum q150r e, by Quorum Technologies (1 mentions) Ultracut uct ultramicrotome, by Leica (1 mentions) Uc6 ultramicrotome, by Leica (1 mentions) Uranyl acetate, by SPI Supplies (1 mentions)

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9 protocols using tecnai g2 12 transmission electron microscope

1

Ultrastructural Analysis of Cells

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The cells were collected by trypsinization and centrifugation and then were fixed with 2.5% glutaraldehyde and 1% osmium tetroxide followed by dehydration in an increasing series of ethanol. The samples were embedded in Durcopan ACM for 6 h, and ultrathin sections were cut using a Leica Ultramicrotome EM UC6, The sections were then stained with uranyl acetate and lead citrate, and examined with a Tecnai G2 12 transmission electron microscope (FEI Company, Holland).
Wang W., Chen L., Shang C., Jin Z., Yao F., Bai L., Wang R., Zhao S, & Liu E. (2020). miR‐145 inhibits the proliferation and migration of vascular smooth muscle cells by regulating autophagy. Journal of Cellular and Molecular Medicine, 24(12), 6658-6669.
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2

Analyzing Mitochondrial Morphology Using TEM

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The mitochondrial morphology was investigated using electron microscopy. Briefly, gastrocnemius muscles were isolated and fixed with 2.5% glutaraldehyde for 1 day. The muscles were washed and cut longitudinally into 0.5 mm thick strips. Following osmification with 2% osmium tetroxide and 1% uranyl acetate en bloc, the stained tissue was routinely dehydrated in a methanol gradient and embedded in Eponate-12. After polymerization, the ultrastructural features of the mitochondria were observed and photographed using a Tecnai G2 12 transmission electron microscope (FEI Company, Holland, The Netherlands).
Liu R., Li H., Fan W., Jin Q., Chao T., Wu Y., Huang J., Hao L, & Yang X. (2017). Leucine Supplementation Differently Modulates Branched-Chain Amino Acid Catabolism, Mitochondrial Function and Metabolic Profiles at the Different Stage of Insulin Resistance in Rats on High-Fat Diet. Nutrients, 9(6), 565.
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3

Visualizing Japanese Encephalitis Virus

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Suspension of purified JEV particles was deposed on carbon-coated 200 mesh copper palladium grids with glow discharged in a Quorum Q150R ES (Quorum). After adhesion for 10 min, the viral particles were chemically fixed with 4% paraformaldehyde in 0.1 M PHEM buffer (pH 7.2) for 10 min. Free aldehydes remained were quenched with 50 mM NH4Cl in PBS for 5 min. The viral particles on the grids were labelled with mouse anti-HA monoclonal antibody (HA-7, Sigma) for 45 min at room temperature, followed by a goat anti-mouse immunoglobulin G (IgG) with 10 nm colloidal gold conjugation (Sigma) for 30 min. After labelling, the grids were negatively stained with aqueous 4% uranyl acetate solution and subsequently observed under a FEI Tecnai G2 12 transmission electron microscope (FEI).
Wang X., Wu Z., Li Y., Yang Y., Xiao C., Liu X., Xiang X., Wei J., Shao D., Liu K., Deng X., Wu J., Qiu Y., Li B, & Ma Z. (2020). p53 promotes ZDHHC1-mediated IFITM3 palmitoylation to inhibit Japanese encephalitis virus replication. PLoS Pathogens, 16(10), e1009035.
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4

Ultrastructural Analysis of Cellular Specimens

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After the indicated treatments, cells in different groups were harvested and fixed in phosphate-buffered saline (PBS) (pH = 7.4) containing 2.5% glutaraldehyde for 2 h at room temperature and then postfixed by use of 1% osmic acid for 1 h at room temperature. The samples were dehydrated in graded ethanol and embedded in epoxy resin. Then, the samples were cut into 70 nm sections using the Ultracut UCT Ultramicrotome (Leica, Wetzlar, Germany). Each section was stained with uranyl acetate and lead citrate for 10 min and observed using the Tecnai G2 12 Transmission Electron Microscope (FEI, Eindhoven, Netherlands).
Wang W., Qing X., Wang B., Ma K., Wei Y, & Shao Z. (2018). Tauroursodeoxycholic Acid Protects Nucleus Pulposus Cells from Compression-Induced Apoptosis and Necroptosis via Inhibiting Endoplasmic Reticulum Stress. Evidence-based Complementary and Alternative Medicine : eCAM, 2018, 6719460.
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5

Transmission Electron Microscopy of Mouse Brain

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Transmission electron microscopy was based on a previously described procedure38 (link). Control and CSDS-exposed mice were anesthetized with sodium pentobarbital (40 mg/kg, i.p.) and perfused with 2.5% glutaraldehyde, then the brains were removed and immersed in the same fixative overnight at 4 °C. After washing with 0.1 M phosphate buffer, the tissues were post-fixed with 1% osmium tetroxide for 5 min at room temperature. The brain sections were then dehydrated in graded ethanol concentrations and embedded in epoxy resin according to standard protocols. Ultrathin sections (400 nm) in the DG region were post-stained with uranyl acetate and lead citrate and finally viewed with an FEI Tecnai G2 12 transmission electron microscope (FEI Company, Hillsboro, Oregon) operated at 80 kV.
Zhang S.Q., Deng Q., Zhu Q., Hu Z.L., Long L.H., Wu P.F., He J.G., Chen H.S., Yue Z., Lu J.H., Wang F, & Chen J.G. (2023). Cell type-specific NRBF2 orchestrates autophagic flux and adult hippocampal neurogenesis in chronic stress-induced depression. Cell Discovery, 9, 90.
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6

Ultrastructural Detection of Cannabinoid and Glutamate Receptors

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Vibratome brain sections were rinsed with 0.1 M PB (pH 7.3), incubated with 1% (w/v) sodium borohydride in PB for 30 min to inactivate free aldehyde groups, rinsed in PB, and then incubated with blocking solution [1% (v/v) normal goat serum (NGS), 4% (w/v) BSA in PB supplemented with 0.02% (w/v) saponin] for 30 min. Sections were incubated with primary antibodies rabbit-anti-CB1 (1:200, CB1-Rb-Af380, Frontier Institute) + guinea pig-anti-VGluT1 (1:400, VGluT1-GP-Af570, Frontier Institute). Sections were rinsed and incubated overnight at 4 °C in the corresponding secondary antibodies: biotinylated goat-anti-guinea pig (VGluT1 detection) + anti-rabbit-IgG coupled to 1.4-nm gold (2003; 1:100 dilution, CB1 detection). Next, sections were processed with an ABC kit, fixed with 0.5% (v/v) osmium tetroxide, and contrasted in 1% (w/v) uranyl acetate. Sections were dehydrated and flat embedded in Durcupan ACM epoxy resin (EMS). Sections were examined and photographed using a Tecnai G2 12 transmission electron microscope (Fei). Specificity of primary antibodies has been previous described (Zhang et al., 2015 (link)).
Mateo Y., Johnson K.A., Covey D.P., Atwood B.K., Wang H.L., Zhang S., Gildish I., Cachope R., Bellocchio L., Guzmán M., Morales M., Cheer J.F, & Lovinger D.M. (2017). Endocannabinoids on cortical terminals orchestrate local modulation of dopamine release in the nucleus accumbens. Neuron, 96(5), 1112-1126.e5.
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7

Transmission Electron Microscopy Protocol

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The cells were treated as indicated. Then, the samples were collected and fixed with 2.5% phosphate-buffered glutaraldehyde. After washing with PBS, the cells were post-fixed with 1% OsO4, dehydrated and embedded in Spurr's resin. Thin sections (~50-70 nm) were cut on a UC6 ultramicrotome (Leica Microsystems, Wetzlar, Germany) and stained with 4% uranyl acetate and lead citrate. Finally, digital images were acquired with a Tecnai G2 12 transmission electron microscope (FEI, Hillsboro, OR, USA).
Zhang Y., Guo C., Liu L., Xu J., Jiang H., Li D., Lan J., Li J., Yang J., Tu Q., Sun X., Alamgir M., Chen X., Shen G., Zhu J, & Tao J. (2020). ZnO-based multifunctional nanocomposites to inhibit progression and metastasis of melanoma by eliciting antitumor immunity via immunogenic cell death. Theranostics, 10(24), 11197-11214.
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8

Ultrastructural Analysis of Heart Tissue

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Heart tissues (1 mm3) were fixed with 4% glutaraldehyde in a 0.1 M phosphate buffer at 4°C for 4 h. Next, the samples were postfixed with 1% osmium tetroxide at room temperature for 2 h. Then, they were embedded in araldite/812 after dehydration in graded ethanol (70–100%). Ultrathin sections were cut and stained with uranyl acetate and lead hydroxide and visualized using a Tecnai G2 12 transmission electron microscope (FEI, Hillsboro, OR, USA), as previously described [17 (link)].
Fang Q., Liu X., Ding J., Zhang Z., Chen G., Du T., Wang Y, & Xu R. (2022). Soluble Epoxide Hydrolase Inhibition Protected against Diabetic Cardiomyopathy through Inducing Autophagy and Reducing Apoptosis Relying on Nrf2 Upregulation and Transcription Activation. Oxidative Medicine and Cellular Longevity, 2022, 3773415.
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9

Transmission Electron Microscopy of Microvesicles

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For transmission electron microscopy, the pelleted MVs were fixed in 2.5% (w/v) glutaraldehyde in PBS, dehydrated and embedded in Epon (SPI Supplies, Inc., West Chester, PA, USA). Ultrathin sections (65-nm) were cut and stained with uranyl acetate (SPI Supplies, Inc.) and Reynold's lead citrate (SPI Supplies, Inc.). The sections were examined in a Tecnai G2 12 transmission electron microscope (FEI, Hillsboro, OR, USA).
Chen X., Xiong W, & Li H. (2016). Comparison of microRNA expression profiles in K562-cells-derived microvesicles and parental cells, and analysis of their roles in leukemia. Oncology Letters, 12(6), 4937-4948.
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