Cytokine levels were measured in mouse serum (n = 2–5/group) as previously described [61 (link)]. Briefly, serum samples were analyzed using Q-Plex™ Mouse Cytokine Screen (16-plex), Quansys Imager, and Quansys reagents (Quansys Biosciences, Logan, Utah). Cytokine levels are expressed as pg/mL and presented as the mean ± SEM in Supplementary Table S2 .
Quansys imager
Manufactured by Quansys Biosciences
Sourced in United States
The Quansys Imager is a versatile laboratory equipment designed for the detection and quantification of various analytes. It utilizes advanced imaging technologies to capture and analyze data from a wide range of assay formats, including multiplex assays, enzyme-linked immunosorbent assays (ELISAs), and other immunoassays. The Quansys Imager provides accurate and reliable results, enabling researchers and scientists to obtain insights into their samples with efficiency and precision.
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Lab products found in correlation
6 protocols using quansys imager
1
Cytokine Profiling in Mouse Serum
2
Simultaneous Cytokine and Chemokine Profiling
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Levels of human cytokines IL-1β, IL-2, IL-4, IL-5, IL-6, IL-10, IL-13, IL-17, IL-23 IFNγ and TNFα and chemokines GROα, Eotaxin, I-309, IP-10, MCP-1 and MCP-2 were detected using Q-Plex technology (Quansys Biosciences, Logan, UT, USA) according to the manufacturer’s instructions. Readouts were obtained with a Quansys Imager (Quansys Biosciences) and results analyzed using the Q-View Software program (Quansys Biosciences). A human sL-Selectin Instant ELISA (eBioscience) was also performed according to the manufacturer’s recommended protocol, with the exception that culture supernatants were diluted 1:10, of which 50 μL was added to provide plates in duplicate.
3
Cytokine Profiling in Whole Blood
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Levels of human cytokines were measured in whole blood culture supernatants using Q-Plex technology (Quansys Biosciences) according to the manufacturer’s instructions. Readouts were obtained with a Quansys Imager (Quansys Biosciences), and results were analyzed using the Q-View Software program (Quansys Biosciences).
4
Quantifying Mouse Cytokine Profiles
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Twelve cytokines (IFNγ, IL-1a, IL-1b, IL-2, IL-4, IL-5, IL-6, IL-10, IL-12, KC, MIP-2, TNFα,) were measured in mouse plasma and brain using the Quansys Imager and the Quansys reagents (Quansys Biosciences, Logan, Utah). Cytokines chemiluminescence assays were conducted at the Institute for Neuroimmune Medicine, Nova Southeastern University, Fort Lauderdale, FL). Cytokines in brain and plasma (1-month post DFP) are expressed as pg/ml. Method details have been previously reported [37 (link),38 (link)].
5
Quantitative Cytokine Detection via Q-Plex
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Levels of human cytokines were detected using Q-Plex technology (Quansys Biosciences, Logan, UT, USA) according to manufacturer's instructions. Briefly, 30 μL of each sample (duplicate) was diluted 1:2 in diluent buffer, and a total volume of 50 μL of diluted sample and control standards was added to a 96-well v-bottom binding plate. Plates were then incubated for 1 h on a shaker at room temperature in the dark. Following incubation, plates were washed and a volume of 50 μL of detection mix was added to each well, and incubated for 1 h. Next, plates were washed and a volume of 50 μL streptavidin HRP was added to each well and incubated for additional 15 min. Finally, plates were washed and a volume of 50 μL substrate mix was added to each well. Readouts were obtained with a Quansys Imager (Quansys Biosciences, Logan, UT, USA) and results analyzed using the Q-View Software program (Quansys Biosciences).
6
Cytokine and Chemokine Profiling in SUDV Response
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The levels of secreted cytokines and chemokines were analyzed as previously described [13 ]. Cytokine and chemokines IL-2, IL-4, IL-5, IP-10, IFN-γ, and TNF-α were measured in the culture medium of PBMCs following SUDV and SUDV proteins GP, NP, VP30 and VP40 stimulation using Q-Plex technology (Quansys Biosciences, Logan, Utah, USA) according to the manufacturer's instructions. Data were collected with a Quansys Imager (Quansys Biosciences) and results analyzed using Q-View (Quansys Biosciences).
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