Spss statistical program version 18

Descriptive statistics (frequencies, percentages, means and standard deviation) were calculated, excluding missing data from the analysis. Convenience and preference scores were calculated based on mothers, MCH’s and hospital staff’s feedback. A container was assigned convenience score of ‘2’ if it was selected as most convenient to use, a score of ‘1’ if it was selected as convenient and a score of ‘0’ if it was difficult to use. Preference scores were assigned based on the preference/choice of container. A score of “2” for first preference; “1” for second preference, and the non-preferred container received a score of ‘0’. Data were analyzed using SPSS Statistical Program Version 18.0 (SPSS, Chicago, IL).

All data were collected from at least three independent experiments and presented as mean ± S.E.M. Comparisons of data from various behavioral tests and western blot assay between defeated and control groups were evaluated via two independent samples t-test. Data among multiple groups were analyzed using one-way analysis of variance (ANOVA) with the least significant difference test for pairwise comparison. A P-value of 0.05 was considered to be the critical cutoff value of statistical significance. All data analyses were carried out using the SPSS statistical program version 18.0 (SPSS Inc., Chicago, IL, USA).