Barley glucan

Starch was purchased from Merck (Germany). Yeast extract, peptone, gelatin, mono- and disaccharides, barley glucan, birchwood and beechwood xylan, carboxymethyl cellulose (CMC), microcrystalline cellulose (MCC) Avicel, inulin, cellobiose, dextrin, dextran, pullulan, laminarin, lichenan, pectin, and alginate were purchased from Sigma Aldrich (Taufkirchen, Germany), or kindly provided by Dr. R. Wohlgemuth. Agarose (agarose MP) was purchased from Boehringer (Mannheim, Germany) and chitin (crab chitin) from Bioprogress (Russia). Chitin and chitosan were kindly provided by Dr. S. Lopatin from the Centre of Bioengineering, Research Center of Biotechnology, RAS, Moscow, Russia. Amorphous chitin (AMCH) and amorphous cellulose (AMC) for growth experiments and native activity measurements were prepared according to Sorokin et al. (2015) (link). Other polysaccharides, such as glucomannan, galactomannan, arabinoxylan, and curdlan, were purchased from Megazyme (Ireland). Bamboo leaves collected near the sampling site were dried at room temperature and used as the growth substrate.

Restriction enzymes Xba I, Hind III and T4 ligase were purchased from Fermentas (Thermo Fisher Scientific, Germany). DNA markers and protein markers were purchased from LabLead Co., Ltd (Beijing, China). PCR mix was purchased from JiErDun Co., Ltd (Shanghai, China). Salmon sperm DNA, hemin, and TMB were purchased from TaiTianHe Co., Ltd (Jinan, China). A turbidimetric (1,3)-β-D-glucan test kit was purchased from Bokang Marine Biological Company (Zhanjiang, China). Barley glucan, curdlan, mannan, endotoxin, dextran, and (1,3)-β-D-glucanase were sourced from Sigma–Aldrich (St. Louis, MO). ssDNA libraries and primers were synthesized by Sangon Biotech Co., Ltd (Shanghai China).