Gb11290

Manufactured by Wuhan Servicebio Technology

Sourced in China

GB11290 is a laboratory equipment product. It is designed to perform a core function, but a detailed description while maintaining an unbiased and factual approach cannot be provided based on the information available.

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Lab products found in correlation

Hm340e, by Thermo Fisher Scientific (1 mentions) Gb111364, by Wuhan Servicebio Technology (1 mentions) Gb11081, by Wuhan Servicebio Technology (1 mentions) Gb12063, by Wuhan Servicebio Technology (1 mentions) Gb111499, by Wuhan Servicebio Technology (1 mentions)

2 protocols using gb11290

Immunostaining of HIF1α and Cell Markers

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The tissue specimens were immersed in formalin for 24 hours, and then embedded in paraffin and sectioned (paraffin microtome, HM340E, Thermo FIsher Scientific). After deparaffinization and antigen retrieval, the slides were blocked with 10% normal goat serum for 30 minutes at room temperature, and incubated with anti-HIF1α (36169T, Cell Signaling Technology) antibody solution for 24 hours at 4°C with gentle shaking. The images were scanned with a pathology section scanner (Pannoramic MIDI) after DAB staining. For dual immunofluorescent staining, mouse anti-CD31 (GB12063, Servicebio) antibody was incubated with rabbit anti–α-SMA (GB111364, Servicebio), anti–claudin 5 (GB11290, Servicebio), and anti-desmin (GB11081, Servicebio) antibody solution for 24 hours at 4°C with gentle shaking. The images were scanned after incubation with mouse-derived (red) and rabbit-derived (green) fluorescent secondary antibodies. The staining intensity was analyzed with ImageJ (NIH).

Cui J., Che Z., Zou L., Chen D., Xie Z., Ding K., Jiang H., Li A., Zhou J, & Shu Y. (2023). JP1 normalizes tumor vasculature to suppress metastasis and facilitate drug delivery by inhibiting IL-8. JCI Insight, 8(12), e161675.

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Immunohistochemical Analysis of LUAD

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Briefly, the LUAD samples of 45 pN2 stage patients were dewaxed, antigen repaired, treated with hydrogen peroxide, blocked, incubated with specific primary antibodies (anti-Ki67 antibody: GB111499, Servicebio, Wuhan, China; anti-CLDN5 antibody: GB11290, Servicebio, Wuhan, China; and anti-NKG7 antibody: PA5-67173, Invitrogen, Carlsbad, CA, USA) and horseradish peroxidase (HRP)-labeled secondary antibody, followed by staining, counterstaining, and microscopy.

Zhu J., Wang W., Ma Y., Zhao J, & Xiong Y. (2023). Development and validation of a gene-based classification model for pN2 lung adenocarcinoma. Translational Lung Cancer Research, 12(3), 494-509.

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