Hesi 2 electrospray ionization probe

Manufactured by Thermo Fisher Scientific

The HESI-II electrospray ionization probe is a component used in mass spectrometry instrumentation. Its core function is to convert analytes in liquid form into gas-phase ions, which can then be detected and analyzed by the mass spectrometer.

Automatically generated - may contain errors

Lab products found in correlation

Q exactive orbitrap mass spectrometer, by Thermo Fisher Scientific (2 mentions) Tracefinder 4, by Thermo Fisher Scientific (2 mentions) Vanquish ultra high performance liquid chromatography system, by Thermo Fisher Scientific (2 mentions) Zic philic stationary phase, by Merck Group (2 mentions)

Spelling variants of this product

HESI-II (35 citations) HESI II probe (34 citations) HESI) probe (12 citations) HESI-II probe for electrospray ionization (5 citations) Heated Electrospray Ionization (HESI-II) Probe (2 citations)

2 protocols using hesi 2 electrospray ionization probe

Quantitative Metabolite Profiling by LC-MS

Check if the same lab product or an alternative is used in the 5 most similar protocols

LC-MS analysis was performed on a Vanquish ultra-high-performance liquid chromatography system coupled to a Q Exactive orbitrap mass spectrometer by a HESI-II electrospray ionization probe (Thermo). External mass calibration was performed weekly. Metabolite samples (2.5 μL) were separated using a ZIC-pHILIC stationary phase (2.1×150 mm, 5 μm; Merck). The autosampler temperature was 4 °C and the column compartment was maintained at 25 °C. Mobile phase A was 20 mM ammonium carbonate and 0.1% ammonium hydroxide. Mobile phase B was acetonitrile. The flow rate was 0.1 mL/min. Solvent was introduced to the mass spectrometer via electrospray ionization with the following source parameters: sheath gas 40, auxiliary gas 15, sweep gas 1, spray voltage +3.0 kV for positive mode and –3.1 kV for negative mode, capillary temperature 275 °C, S-lens RF level 40, and probe temperature 350 °C. Data were acquired and peaks integrated using TraceFinder 4.1 (Thermo).

Copeland C.A., Olenchock B.A., Ziehr D., McGarrity S., Leahy K., Young J.D., Loscalzo J, & Oldham W.M. (2023). MYC overrides HIF-1α to regulate proliferating primary cell metabolism in hypoxia. eLife, 12, e82597.

+ Open protocol

+ Expand

Accurate LC-MS Metabolite Profiling Workflow

Check if the same lab product or an alternative is used in the 5 most similar protocols

LC-MS analysis was performed on a Vanquish ultra-high-performance liquid chromatography system coupled to a Q Exactive orbitrap mass spectrometer by a HESI-II electrospray ionization probe (Thermo). External mass calibration was performed weekly. Metabolite samples (2.5 μL) were separated using a ZIC-pHILIC stationary phase (2.1 x 150 mm, 5 μm) (Merck). The autosampler temperature was 4 °C and the column compartment was maintained at 25 °C. Mobile phase A was 20 mM ammonium carbonate and 0.1% ammonium hydroxide. Mobile phase B was acetonitrile. The flow rate was 0.1 mL/min. Solvent was introduced to the mass spectrometer via electrospray ionization with the following source parameters: sheath gas 40, auxiliary gas 15, sweep gas 1, spray voltage +3.0 kV for positive mode and −3.1 kV for negative mode, capillary temperature 275 °C, S-lens RF level 40, and probe temperature 350 °C. Data were acquired and peaks integrated using TraceFinder 4.1 (Thermo).

Diaz-Cuadros M., Miettinen T.P., Skinner O.S., Sheedy D., Díaz-García C.M., Gapon S., Hubaud A., Yellen G., Manalis S.R., Oldham W.M, & Pourquié O. (2023). Metabolic regulation of species-specific developmental rates. Nature, 613(7944), 550-557.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!