Axio observer 7

Manufactured by Lumencor

The Axio Observer 7 is a high-performance inverted fluorescence microscope designed for advanced imaging applications. It features a stable and ergonomic design, as well as a range of optical and illumination options to support various imaging techniques.

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Lab products found in correlation

Flash 4, by Hamamatsu Photonics (2 mentions) Spectra x led, by Hamamatsu Photonics (1 mentions)

2 protocols using axio observer 7

Visualizing Viral Infection in PANC-1 Cells

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PANC-1 cells were seeded in a 6-well plate at 6 × 10^{^5} cells per well. Following overnight attachment, the cells were infected with CVB3, LACV, and SINV at an MOI of 1 or left uninfected for 72 h. Pictures of the cells were taken with a Levenhuk M500 Base camera with LevenhukLite software (Version ×64). Persistently infected PANC-1 cells were plated at the same density, and photos were taken as previously described. For immunofluorescence images, PANC-1 cells were seeded to coverslips and fixed 24 h later. Fixed cells were then stained for nuclei (DAPI) and dsRNA (J2, Invitrogen) as previously described (32 (link)). They were imaged on Zeiss Axio Observer 7 with Lumencor Spectra X LED light system and a Hamamatsu Flash 4 camera using Zen Blue software with a 40× objective.

Mastrodomenico V., LoMascolo N.J., Firpo M.R., Villanueva Guzman M.D., Zaporowski A, & Mounce B.C. (2023). Persistent Coxsackievirus B3 Infection in Pancreatic Ductal Cells In Vitro Downregulates Cellular Polyamine Metabolism. mSphere, 8(3), e00036-23.

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Immunofluorescence Analysis of ACE2 and CEACAM

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Cells grown on cover slips were either treated with 1 mM DFMO or untreated. Cells were fixed with 4% formalin for 15 minutes, washed with PBS, permeabilized and blocked with 0.2% Triton X-100 and 2% BSA in PBS (blocking solution) for 30 minutes at room temperature (RT). Cells were sequentially incubated as follows: Primary rabbit anti-hACE2 (Invitrogen, SN0754) (Vero E6) or rabbit anti-mCEACAM (BHK-R) antibodies 1:500 in blocking for 2 hrs at room temperature. Cells were subsequently washed then incubated with secondary goat anti-rabbit (1:500 in blocking, 30 min, RT). Mounting media with DAPI was used to visualize nuclei. Samples were imaged with Zeiss Axio Observer 7 with Lumencor Spectra X LED light system and a Hamamatsu Flash 4 camera using appropriate filters using Zen Blue software with a 40X objective.

Firpo M.R., Mastrodomenico V., Hawkins G.M., Prot M., Levillayer L., Gallagher T., Simon-Loriere E, & Mounce B.C. (2020). Targeting polyamines inhibits coronavirus infection by reducing cellular attachment and entry. ACS infectious diseases, 7(6), 1423-1432.

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