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Ique plus flow cytometer

Manufactured by Intellicyt
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The IQue Plus is a flow cytometer designed for rapid and high-throughput analysis of biological samples. It employs fluidics, optics, and a digital signal processing system to detect and analyze the physical and fluorescent properties of individual cells or particles suspended in a fluid stream. The core function of the IQue Plus is to provide researchers with a versatile tool for cell-based assays, immunophenotyping, and other applications that require quantitative analysis of heterogeneous cell populations.

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Lab products found in correlation

Alexa fluor 647 dye, by Thermo Fisher Scientific (2 mentions)

Close spelling variants of this product

IQue Screener Plus flow cytometer (26 citations) IQue Flow Cytometer (2 citations)

2 protocols using ique plus flow cytometer

1

Henipavirus RBP Protein Expression

Check if the same lab product or an alternative is used in the 5 most similar protocols
A construct containing cDNA encoding full-length HeV-RBP protein was transfected using polyethylenimine into 293F cells, and cells were cultured at 37°C in 5% CO2 for 3 days. Cells subsequently were plated at 50,000 cells/well in V-bottom 96-well plates, washed, and incubated with either 20 μg/mL primary mAb in 30 μL or FACS buffer alone for 30 minutes at 4°C. Without washing, 30 μL serially diluted mAb labeled with Alexa Fluor 647 dye (ThermoFisher) was added to wells and incubated for 30 minutes at 4°C. Cells were washed and resuspended in FACS buffer and analyzed using an iQue Plus flow cytometer (Intellicyt). Dead cells were excluded from analysis by fluorescent staining with 4′,6-diamidino-2-phenylindole (DAPI).
Doyle M.P., Kose N., Borisevich V., Binshtein E., Amaya M., Nagel M., Annand E.J., Armstrong E., Bombardi R., Dong J., Schey K.L., Broder C.C., Zeitlin L., Kuang E.A., Bornholdt Z.A., West B.R., Geisbert T.W., Cross R.W, & Crowe JE J.r. (2021). Cooperativity mediated by rationally selected combinations of human monoclonal antibodies targeting the henipavirus receptor binding protein. Cell reports, 36(9), 109628.
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2

Henipavirus RBP Protein Expression

Check if the same lab product or an alternative is used in the 5 most similar protocols
A construct containing cDNA encoding full-length HeV-RBP protein was transfected using polyethylenimine into 293F cells, and cells were cultured at 37°C in 5% CO2 for 3 days. Cells subsequently were plated at 50,000 cells/well in V-bottom 96-well plates, washed, and incubated with either 20 μg/mL primary mAb in 30 μL or FACS buffer alone for 30 minutes at 4°C. Without washing, 30 μL serially diluted mAb labeled with Alexa Fluor 647 dye (ThermoFisher) was added to wells and incubated for 30 minutes at 4°C. Cells were washed and resuspended in FACS buffer and analyzed using an iQue Plus flow cytometer (Intellicyt). Dead cells were excluded from analysis by fluorescent staining with 4′,6-diamidino-2-phenylindole (DAPI).
Doyle M.P., Kose N., Borisevich V., Binshtein E., Amaya M., Nagel M., Annand E.J., Armstrong E., Bombardi R., Dong J., Schey K.L., Broder C.C., Zeitlin L., Kuang E.A., Bornholdt Z.A., West B.R., Geisbert T.W., Cross R.W, & Crowe JE J.r. (2021). Cooperativity mediated by rationally selected combinations of human monoclonal antibodies targeting the henipavirus receptor binding protein. Cell reports, 36(9), 109628.
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