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Cd16 fitc 3gb

Manufactured by Beckman Coulter
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CD16-FITC (3G8) is a fluorochrome-conjugated monoclonal antibody that binds to the CD16 antigen. CD16 is expressed on natural killer cells, neutrophils, and a subset of monocytes. This product is intended for research use only and its core function is to facilitate the identification and enumeration of CD16-positive cell populations using flow cytometry.

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Lab products found in correlation

Cd56 bv605 hcd56, by BioLegend (2 mentions) Lsrfortessa, by BD (2 mentions) Tigit apc, by Thermo Fisher Scientific (1 mentions) Nkg2a pe vio770, by Miltenyi Biotec (1 mentions) 1 step fix lyse solution, by Thermo Fisher Scientific (1 mentions) Cd226 pe, by BioLegend (1 mentions) Cd3 percp ucht1, by BioLegend (1 mentions) Cd25 bv421 m a251, by BD (1 mentions)

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CD16-FITC (7 citations)

2 protocols using cd16 fitc 3gb

1

NK Cell Activation Phenotyping

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Sorted NK cells were plated at a density of 2.5×105 cells/mL in cRPMI in a 24 well plate, and stimulated for 24 hours with IL-2 (100 U/mL; NCI), IL-12 (1 ng/mL), and/or IL-18 (10 ng/mL). Cells were stained with antibodies against the following markers: CD56-BV605 (HCD56; BioLegend), NKG2D-BV711 (1D11; BioLegend), CD226-PE (11A8; BioLegend), CD16-FITC (3GB; Beckman Coulter), CD25-BV421 (M-A251; BD Biosciences), CD57-APC-Vio770 (TB03; Miltenyi Biotec), NKG2A-PE-Vio770 (REA110; Miltenyi Biotec) and TIGIT-APC (MBSA43; eBioscience), as well as for fluorescence minus one (FMO) controls. Data were collected on a BD LSRFortessa or an Accuri C6, and analyzed using FlowJo software V9 (TreeStar Inc).
Dean J.W., Peters L.D., Fuhrman C.A., Seay H.R., Posgai A.L., Stimpson S.E., Brusko M.A., Perry D.J., Yeh W.I., Newby B.N., Haller M.J., Muir A.B., Atkinson M.A., Mathews C.E, & Brusko T.M. (2020). Innate Inflammation Drives NK Cell Activation to Impair Treg Activity. Journal of autoimmunity, 108, 102417.
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2

Multiparametric Flow Cytometry Profiling

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400 μL of whole blood was directly stained for CD3-PerCP (UCHT1; Biolegend), CD16-FITC (3GB; Beckman Coulter), CD25-BV421 (M-A251; BD Biosciences), CD56-BV605 (HCD56; BioLegend), CD57-APC-Vio770 (TB03; Miltenyi Biotec), NKG2A-PE-Vio770 (REA110; Miltenyi Biotec), TIGIT-APC (MBSA43; eBioscience), and CD226-PE (11A8; BioLegend) for 30 minutes at room temperature. Red blood cells were lysed using 1-step Fix/Lyse Solution (eBioscience), and cells were washed with FACS buffer. Data were collected on a BD LSRFortessa and analyzed using FlowJo software.
Dean J.W., Peters L.D., Fuhrman C.A., Seay H.R., Posgai A.L., Stimpson S.E., Brusko M.A., Perry D.J., Yeh W.I., Newby B.N., Haller M.J., Muir A.B., Atkinson M.A., Mathews C.E, & Brusko T.M. (2020). Innate Inflammation Drives NK Cell Activation to Impair Treg Activity. Journal of autoimmunity, 108, 102417.
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