Anti arg 1 antibody

Mouse RAW264.7 macrophages were obtained from American Type Culture Collection (Manassas, VA, USA) and maintained in Dulbecco’s modified Eagle’s medium (DMEM) (Hyclone, Logan, UT, USA) containing 10% fetal bovine serum (Gibco, Life Technologies). PDX was purchased from Cayman (Cayman Chemical, Ann Arbor, MI, USA). IL-6, TNF-α, MCP-1 and IL-10 (enzyme-linked immunosorbent assay, ELISA) kits were obtained from RayBiotech (RayBiotech Inc., Norcross, GA, USA). Carboxylate-modified yellowgreen (YG) microspheres were purchased from Invitrogen (Thermo Fisher scientific, Waltham, MA, USA). FITC-anti-F4/80, PE-anti-CD11b and PE-anti-CD206 were obtained from eBioscience (eBioscience, San Diego, CA, USA). Anti-Arg1 antibody was purchased from Abcam (Abcam, Cambridge, MA, USA). Anti-Ym1 antibody was purchased from Stemcell Technologies (Stemcell Technologies, Vancouver, Canada). Anti-PPAR-γ and anti-GAPDH antibodies were obtained from Santa Cruz Biotechnology (Santa Cruz, CA, USA).

Zn(NO₃)₂·6H₂O, dimethyl sulfoxide (DMSO), methanol, phosphate-buffered saline (PBS), 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT), ECL detection kit, and lipopolysaccharide (LPS) were purchased from Solarbio Biochemical Co., Ltd. (China). Fetal bovine serum (FBS), trypsin/EDTA, Dulbecco’s modified Eagle’s medium (DMEM), and penicillin/streptomycin were purchased from Sigma-Aldrich Biotech., Ltd. (USA). A BCA protein assay kit was purchased from Beyotime Biotech (China). FA-PEG (MW = 2,000 Da) was purchased from Fan Shuo Biotech., Shanghai (China). Anti-CD11b antibody (NO. ab184308), anti-Iba-1 antibody (NO. ab178846), anti-Arg-1 antibody (NO. ab239731), anti-iNOS antibody (NO. ab210823), anti-NeuN antibody (NO. ab177487), anti-GFAP antibody (NO. ab7260), anti-MBP antibody (NO. ab218011), and anti-IL-1β antibody (NO. ab254360) were obtained from Abcam (USA). The anti-cleaved-caspase-3 antibody (NO. 9661S) was obtained from Cell Signaling Technology (UK).

Anti-NeuN antibody, anti-CD11b antibody, anti-IL-1β antibody, anti-TNF-α antibody, anti-iNOS antibody, and anti-Arg-1 antibody were obtained from Abcam. Anti-Iba antibody was obtained from Wako. The Anti-Cleaved-Caspase-3 antibody and anti-Bax antibody were obtained from Cell Signaling Technology. The anti-β-Tubulin antibody and secondary antibodies were purchased from Proteintech. Transmission electron microscopy (TEM) and high-resolution transmission electron microscopy (HR-TEM) images were recorded using Tecnai™ G2 F30 Series. Energy-dispersive X-ray spectroscopy (EDS) was performed using JEM 2100F. Inductively coupled plasma mass spectrometry (ICP-MS) was used to determine the concentration of NCs. Zeta potential was studied by Mastersizer3000.