Sulfopropyl sepharose

Manufactured by GE Healthcare

Sourced in Sweden

Sulfopropyl-Sepharose is a chromatography medium used for the purification and separation of biomolecules. It consists of a porous agarose matrix functionalized with sulfopropyl groups, which provide cation exchange properties. This product is commonly used in laboratory settings for the purification of proteins, peptides, and other charged biomolecules.

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Lab products found in correlation

Butyl sepharose, by GE Healthcare (2 mentions) Octyl sepharose cl 4b, by GE Healthcare (1 mentions) Phenyl sepharose cl 4b, by GE Healthcare (1 mentions) Bovine serum albumin (bsa), by Merck Group (1 mentions) Polyethyleneimine, by Merck Group (1 mentions) Docosahexaenoic acid, by Merck Group (1 mentions) Polyallylamine, by Merck Group (1 mentions) P nitrophenyl palmitate, by Merck Group (1 mentions) Burkholderia cepacia lipase, by Merck Group (1 mentions) Thermomyces lanuginosus lipase, by Merck Group (1 mentions) Phenyl sepharose, by GE Healthcare (1 mentions) Q exactive mass spectrometer, by Thermo Fisher Scientific (1 mentions) Superdex 75, by GE Healthcare (1 mentions)

3 protocols using sulfopropyl sepharose

Macaúba Cake and Fish Oil Enzyme Purification

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M. spathulata R25L270 strain was obtained from the culture collection of the Universidade Federal de Minas Gerais (Belo Horizonte, Brazil). Macaúba cake was obtained from Biodiesel Company (Minas Gerais, Brazil). The fish oil was obtained from BTSA, Biotecnologías Aplicadas, S.L. (Madrid, Spain), DEAE-agarose (Diethylaminoethyl-agarose), Octyl-Sepharose™ CL-4B, Phenyl-Sepharose™ CL-4B, Butyl-Sepharose, Sulfopropyl-Sepharose and Cyanogenogen Bromide (BrRCN)(CNBr) supports were obtained from GE Bio-Sciences AB (Uppsala, Sweden). The substrate pNPP and all other chemicals were obtained from Sigma Chemical Co. (St. Louis, MO, USA).

T. de A. Souza L., Moreno-Perez S., Fernández Lorente G., P. Cipolatti E., de Oliveira D., R. Resende R, & C. Pessela B. (2017). Immobilization of Moniliella spathulata R25L270 Lipase on Ionic, Hydrophobic and Covalent Supports: Functional Properties and Hydrolysis of Sardine Oil. Molecules : A Journal of Synthetic Chemistry and Natural Product Chemistry, 22(10), 1508.

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Lipase Immobilization and Characterization

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Agarose 10BCL was purchased from Agarose Beads Technologies (Madrid, Spain). Sulfopropyl sepharose, Butyl Sepharose, Octyl Sepharose and Phenyl Sepharose were purchased from GE Healthcare (Uppsala, Sweden). Immobeads-150 Octadecyl, Immobeads-300 Methyl-Estyrene, Lewatit 1600, Polyethyleneimine, Polyallylamine, p-nitrophenyl palmitate, docosahexaenoic acid, Burkholderia cepacia lipase (Amano), Thermomyceslanuginosus lipase (Novozymes), bovine serum albumin and dextran (Leuconostoc mesenteroides) were obtained from Sigma-Aldrich Co. (St. Louis, IL, USA). Methoxypolyethylene glycol amine (NH₂-PEG) was purchased from Rapp Polymere GmbH (Tubingen, Germany). All other reagents were of analytical grade.

Martins P.A., Trobo-Maseda L., Lima F.A., de Morais Júnior W.G., De Marco J.L., Salum T.F, & Guisán J.M. (2022). Omega-3 production by fish oil hydrolysis using a lipase from Burkholderia gladioli BRM58833 immobilized and stabilized by post-immobilization techniques. Biochemistry and Biophysics Reports, 29, 101193.

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Phenylalanyl-tRNA Synthetase Purification

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Materials were obtained from Merck unless otherwise mentioned. ESI-MS was performed using a Thermo Fisher Scientific Q-Exactive mass spectrometer. Plasmid pKPY514 coding for different subunits of E. coli phenylalanyl-tRNA synthetase (Ec PheRS) was a gift from D. Tirrell (Addgene plasmid #62598; http://n2t.net/addgene:62598; RRID: Addgene_62598) (60 (link)). Superdex 75 and sulfopropyl-Sepharose columns were purchased from GE Healthcare Life Sciences, USA. The nonhydrolyzable analogs of aa-tRNAs were purchased from Jena Biosciences, Germany.

Mazeed M., Singh R., Kumar P., Roy A., Raman B., Kruparani S.P, & Sankaranarayanan R. (2021). Recruitment of archaeal DTD is a key event toward the emergence of land plants. Science Advances, 7(6), eabe8890.

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