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2 protocols using advillin creer

1

Genetic Tools for Somatosensory Neuroscience

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ErbB4-2A-CreERT2 mice (#012360), Ai9 (Rosa-CAG-LSL-tdTomato) reporter mice (#007905), cFos::shEGFP mice (#018306), MrgprD-CreER (#031286), SST-Cre (#013044), CCK-Cre (#012706), Rosa-LSL-EYFP (#006148), Advillin-CreER (#032027) and TRPV1-Cre mice (#017769) were obtained from the Jackson Laboratory. ErbB4f/f mice, αMHC::ErbB4 mice, NRG1f/f, T796G, and stop-ErbB4 mice have been described previously (Garcia-Rivello et al., 2005 (link); Li et al., 2002 (link); Tidcombe et al., 2003 (link); Wang et al., 2018b (link); Wang et al., 2021 ). Mice were housed in an environment of 22 ± 2°C and 12 hr light/dark cycle with food and water ad libitum. Animal experimental procedures were approved by the Institutional Animal Care and Use Committee of Case Western Reserve University. Male mice were used in the present study. For the recording of Aβ, Aδ and C inputs, 20-30 day-old mice were used. For semi-intact recordings, 4-7 week-old mice were used. For recording in spinal cord slices, viral injections were performed in 2-4 week-old mice and recordings were performed 2-3 weeks later. For behavioral studies, mice of 2-5 month-old were used. Experimenters were blinded to genotypes and treatments.
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2

Mouse Genetic Models for Neuroscience Research

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All animal procedures followed the ethical guidelines outlined in the National Institutes of Health Guide for the Care and Use of Laboratory Animals, and all protocols were approved by the institutional animal care and use committee (IACUC) at Harvard Medical School. Mice were maintained under constant temperature (23 ± 1 °C) and relative humidity (46 ± 5%) with a 12-h light:dark cycle. Wild-type C57BL/6J (000664), Nestin-cre (003771), Phox2b-cre (016223), Advillin-creER (032027), Trpv1-IRES-cre (017769), Pdyn-IRES-cre (027958), Piezo2-EGFP-IRES-cre (027719), Oxtr-IRES-cre (031303), Agrp-cre (012899), lsl-DTR (007900), lsl-tdTomato (Ai14, 007914) were purchased from Jackson Laboratories. Gabra1-IRES-cre, lsl-L10-GFP and Ptger3flox mice were previously generated14 (link),22 (link),51 (link).
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