Log In Sign up
The largest database of trusted experimental protocols

Mouse igg anti human igm

Manufactured by Novus Biologicals
Visit Supplier

Mouse IgG anti‐human IgM is a laboratory reagent used to detect and quantify the presence of human immunoglobulin M (IgM) in samples. It is a mouse-derived antibody that specifically binds to human IgM.

Automatically generated - may contain errors

Lab products found in correlation

Ripa buffer, by Thermo Fisher Scientific (4 mentions) Streptavidin hrp, by R&D Systems (2 mentions) Rabbit igg anti human mouse c1q, by Thermo Fisher Scientific (2 mentions) Rabbit igg anti factor b, by Thermo Fisher Scientific (2 mentions) Donkey anti mouse igg hrp, by Thermo Fisher Scientific (2 mentions) Chemi imager, by Azure Biosystems (2 mentions) Mini protean tgx gel, by Bio-Rad (2 mentions) Goat anti rabbit igg hrp, by Thermo Fisher Scientific (1 mentions)

Close spelling variants of this product

Biotinylated mouse IgG anti-human IgM

2 protocols using mouse igg anti human igm

1

Western Blot Analysis of Complement Proteins

Check if the same lab product or an alternative is used in the 5 most similar protocols
Serum samples were incubated in RIPA buffer (Thermofisher) supplemented with protease inhibitor (Pierce). Total protein was quantified using BSA protein assay kit (Pierce). Proteins were resolved in Mini‐PROTEAN TGX gels (Bio‐Rad) and transferred to polyvinylidene membrane (Immobilion). C1q was detected with rabbit IgG anti‐human/mouse C1q (Invitrogen). Factor B was detected with rabbit IgG anti‐factor B (Invitrogen). Human IgM was detected with mouse IgG anti‐human IgM (Novusbio) with goat anti rabbit‐IgG‐HRP or donkey anti‐mouse‐IgG‐HRP (Invitrogen) as a secondary antibody. Streptavidin‐HRP (R&D systems) and crescendo western HRP substrate (Immobilon) were used prior to acquisition with Chemi‐Imager (Azure Biosystems).
Dahmani M., Cook J.H., Zhu J.C, & Riley S.P. (2021). Contribution of classical complement activation and IgM to the control of Rickettsia infection. Molecular Microbiology, 116(6), 1476-1488.
+ Open protocol
+ Expand
2

Quantification of Complement Proteins

Check if the same lab product or an alternative is used in the 5 most similar protocols
Serum samples were incubated in RIPA buffer (Thermofisher) supplemented with protease inhibitor (Pierce). Total protein was quantified using BSA protein assay kit (Pierce). Proteins were resolved in Mini-PROTEAN TGX gels (Bio-Rad) and transferred to polyvinylidene membrane (Immobilion). C1q was detected with rabbit IgG anti-human/mouse C1q (Invitrogen). Factor B was detected with rabbit IgG anti-factor B (Invitrogen). Human IgM was detected with mouse IgG anti-human IgM (Novusbio) with goat anti rabbit-IgG-HRP or donkey anti-mouse-IgG-HRP (Invitrogen) as a secondary antibody. Streptavidin-HRP (R&D systems) and crescendo western HRP substrate (Immobilon) were used prior to acquisition with Chemi-Imager (Azure Biosystems).
Dahmani M., Cook J.H., Zhu J.C, & Riley S.P. (2021). Contribution of classical complement activation and IgM to the control of Rickettsia infection. Molecular Microbiology, 116(6), 1476-1488.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!