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Cd62l pacific blue

Manufactured by BioLegend
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CD62L Pacific Blue is a fluorochrome-conjugated antibody that binds to the CD62L (L-selectin) cell surface marker. CD62L is expressed on various leukocyte subsets and plays a role in cell adhesion and migration. The Pacific Blue fluorochrome provides a distinct emission spectrum suitable for flow cytometry analysis.

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Lab products found in correlation

Cd45 buv395, by BD (3 mentions) Cd44 fitc, by BioLegend (2 mentions) Efluor 506, by Thermo Fisher Scientific (2 mentions) Cd4 pe cy7, by BioLegend (2 mentions) Cd19 apc cy7, by BioLegend (2 mentions) Cd8b precp cy5, by BioLegend (2 mentions) Klrg1 fitc, by BioLegend (2 mentions) Cd127 alexa700, by Thermo Fisher Scientific (2 mentions) Lsrii flow cytometer, by FlowJo (2 mentions) Cd44 pe, by BioLegend (2 mentions) Foxp3 transcription factor staining buffer set, by Thermo Fisher Scientific (2 mentions) Brefeldin a, by BioLegend (2 mentions) Anti tnf α bv605, by BD (2 mentions) Anti ifn γ alexa 647, by BD (2 mentions) Cd8 apc, by BioLegend (1 mentions) Cd4 fitc, by BioLegend (1 mentions) Cd44 apc cy7, by BioLegend (1 mentions) Facsaria, by BD (1 mentions) Fcs express v3, by De Novo Software (1 mentions) Pd1 pe cy7, by BioLegend (1 mentions)

Close spelling variants of this product

CD62L

7 protocols using cd62l pacific blue

1

Multicolor Flow Cytometry Analysis

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Direct immunofluorescence cell staining was performed using the following cell surface marker antibodies: CD4-FITC, CD8-APC, CD44-APC/Cy7, and CD62L-PacificBlue (all BioLegend, San Diego, CA). Stained cells were analyzed using a FACSAria (Beckton-Dickinson, Mountain view, CA). FACS data were analyzed using FCS Express V3 (De Novo Software, Los Angeles, CA) software.
Li H.H., Wang Y.W., Chen R., Zhou B., Ashwell J.D, & Fornace AJ J.r. (2015). Ionizing Radiation Impairs T Cell Activation by Affecting Metabolic Reprogramming. International Journal of Biological Sciences, 11(7), 726-736.
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2

Flow Cytometric Analysis of T Cell Subsets

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Spleen cells were obtained from non-immunized mice, self-cured mice 25 days post P. yoelii 17X infection and immunized mice with rexPy or rexC on day 20 after the second immunization, as described above. Splenocytes were analyzed for the expression of different markers with an LSRFortessa flow cytometer and data were analyzed with FlowJo software. T cells subsets were identified according to lymphocytes' SSC-A/FSC-A profile and labeling with CD4-PerCp or CD8-Alexa Fluor-700 antibodies (Biolegend). The phenotype of CD4+ and CD8+T cells in spleen was analyzed with a panel of fluorochrome-conjugated antibodies obtained from Biolegend that included: CD62L-Pacific Blue, PD1-PE/Cy7, CD127-PE and CD44-FITC.
Martín-Jaular L., de Menezes-Neto A., Monguió-Tortajada M., Elizalde-Torrent A., Díaz-Varela M., Fernández-Becerra C., Borras F.E., Montoya M, & del Portillo H.A. (2016). Spleen-Dependent Immune Protection Elicited by CpG Adjuvanted Reticulocyte-Derived Exosomes from Malaria Infection Is Associated with Changes in T cell Subsets' Distribution. Frontiers in Cell and Developmental Biology, 4, 131.
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3

Assessing ZIKV-specific T-cell responses in mice

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Freshly-isolated mouse splenocytes were stimulated with an H-2Db-restricted immunodominant ZIKV peptide (amino acids 294–302), with rat anti-mouse CD3 used as a positive control and medium as negative control, for 12 h at 37°C before brefeldin A (BioLegend, 420601) was added for an additional 4 h. Subsequently, single-cell suspensions were blocked for FcγR binding (BioLegend; clone 93) and stained with the following antibodies: CD45 BUV395 (BD BioSciences clone30-F11), CD62L Pacific Blue, KLRG1 FITC, CD44 PE, CD4 PE-Cy7, CD8b PreCP-Cy5.5, CD19 APC-Cy7 (BioLegend clones MEL-14, 2F1/KLRG1, IM7, GK1.5, YTS156.7.7, 6D5, respectively), CD127 Alexa700 (eBioScience clone A7R34), and fixable viability dye (eFluor 506, eBioscience). Subsequently, cells were fixed and permeabilized with Foxp3/Transcription Factor Staining Buffer Set (eBiosciences, 00–5523-00) followed by intracellular staining with anti-TNF-α BV605 and anti-IFN-γ Alexa 647 (BD Biosceinces clones MP6-XT22 and XMG1.2, respectively). Datasets were acquired on a LSRII flow cytometer and analyzed using FlowJo software X 10.0.7.
Hassan A.O., Dmitriev I.P., Kashentseva E.A., Zhao H., Brough D.E., Fremont D.H., Curiel D.T, & Diamond M.S. (2019). A Gorilla Adenovirus-Based Vaccine against Zika Virus Induces Durable Immunity and Confers Protection in Pregnancy. Cell reports, 28(10), 2634-2646.e4.
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4

Multi-Marker Immune Cell Profiling

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Isolated islet and LN cells were surface stained with CD45 BUV395 (BD), CD4 BV711 (BioLegend), CD8 APC-780 (eBioscience), CD62L Pacific Blue (BioLegend), and CD44 FITC (BioLegend) for 30 min on ice.
Lindsay R.S., Whitesell J.C., Dew K.E., Rodriguez E., Sandor A.M., Tracy D., Yannacone S.F., Basta B.N., Jacobelli J, & Friedman R.S. (2021). MERTK on mononuclear phagocytes regulates T cell antigen recognition at autoimmune and tumor sites. The Journal of Experimental Medicine, 218(10), e20200464.
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5

Immunophenotyping of ECAR-armed T Cells

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Isolated T cells were stained with fluorochrome-labeled mabs directed against human CD4/VioBlue (Miltenyi Biotec, clone VIT4), CD3/PE-Cy7 (Biolegend, San Diego, USA, clone UCHT1), CD8/APC (BD Bioscience, clone RPA-T8), CD27/PE (BD Bioscience, clone M-T271) and CD62L/PacificBlue (Biolegend, clone DREG-56). For detection of ECAR surface expression, T cells were incubated with anti-La mab 5B9 [16] and subsequently stained with PE-labeled goat anti-mouse IgG (Beckmann Coulter, Krefeld, Germany). Samples were analyzed using the MACSQuant Analyzer and the MACSQuantify software (Miltenyi Biotec). In order to assess expansion rates of ECAR armed T cells, absolute T cell numbers were quantified using a MACSQuant Analyzer and MACSQuantify software (Miltenyi Biotec) as described elsewhere [25] (link).
Cartellieri M., Koristka S., Arndt C., Feldmann A., Stamova S., von Bonin M., Töpfer K., Krüger T., Geib M., Michalk I., Temme A., Bornhäuser M., Lindemann D., Ehninger G, & Bachmann M.P. (2014). A Novel Ex Vivo Isolation and Expansion Procedure for Chimeric Antigen Receptor Engrafted Human T Cells. PLoS ONE, 9(4), e93745.
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6

Multicolor Flow Cytometry and Live Imaging of T Cells

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For flow cytometric analysis, cells were stained with antibodies against CD8-PE/Cy7, CD45.2-APC750, CD45.1-PerCP/Cy5.5 (eBioscience), Vα11-FITC, CD24-PE, CD62L-Pacific Blue, CD69-PE, CCR7-APC, CD4-APC, (Biolegend). For LFA-1 localization, purified CD4+ T cells were stained with anti-CD44-Alexa488 and anti-CD11a-Alexa647 (M17/4)) and fixed with 4% paraformaldehyde. For live imaging, staining with anti-CD11a-Alexa647 (M17/4)), and anti-CD11a-Alexa546 (2D7) was performed during imaging, at a concentration of 0.08 ng/mL to prevent integrin blockade.
Xu X., Jaeger E.R., Wang X., Lagler-Ferrez E., Batalov S., Mathis N.L., Wiltshire T., Walker J.R., Cooke M.P., Sauer K, & Huang Y.H. (2014). Mst1 Directs Myosin IIa Partitioning of Low and Higher Affinity Integrins during T Cell Migration. PLoS ONE, 9(8), e105561.
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7

Assessing ZIKV-specific T-cell responses in mice

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Freshly-isolated mouse splenocytes were stimulated with an H-2Db-restricted immunodominant ZIKV peptide (amino acids 294–302), with rat anti-mouse CD3 used as a positive control and medium as negative control, for 12 h at 37°C before brefeldin A (BioLegend, 420601) was added for an additional 4 h. Subsequently, single-cell suspensions were blocked for FcγR binding (BioLegend; clone 93) and stained with the following antibodies: CD45 BUV395 (BD BioSciences clone30-F11), CD62L Pacific Blue, KLRG1 FITC, CD44 PE, CD4 PE-Cy7, CD8b PreCP-Cy5.5, CD19 APC-Cy7 (BioLegend clones MEL-14, 2F1/KLRG1, IM7, GK1.5, YTS156.7.7, 6D5, respectively), CD127 Alexa700 (eBioScience clone A7R34), and fixable viability dye (eFluor 506, eBioscience). Subsequently, cells were fixed and permeabilized with Foxp3/Transcription Factor Staining Buffer Set (eBiosciences, 00–5523-00) followed by intracellular staining with anti-TNF-α BV605 and anti-IFN-γ Alexa 647 (BD Biosceinces clones MP6-XT22 and XMG1.2, respectively). Datasets were acquired on a LSRII flow cytometer and analyzed using FlowJo software X 10.0.7.
Hassan A.O., Dmitriev I.P., Kashentseva E.A., Zhao H., Brough D.E., Fremont D.H., Curiel D.T, & Diamond M.S. (2019). A Gorilla Adenovirus-Based Vaccine against Zika Virus Induces Durable Immunity and Confers Protection in Pregnancy. Cell reports, 28(10), 2634-2646.e4.
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